3NPS

Crystal structure of membrane-type serine protease 1 (MT-SP1) in complex with the Fab Inhibitor S4

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
IMGT Antibody Annotation
SAbDab Antibody Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3npsa_	All beta proteins	Trypsin-like serine proteases	Trypsin-like serine proteases	Eukaryotic proteases	Matriptase MTSP1	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d3npsb_	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d3npsc2	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	C1 set domains (antibody constant domain-like)	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d3npsc1	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Trypsin-like serine proteases	8035635	3000114	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Trypsin_1	e3npsA1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel 2	F: Trypsin_1	ECOD (1.6)
B	C1-set_1	e3npsB2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
C	C1-set_4	e3npsC2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.40.10.10	Mainly Beta	Beta Barrel	Thrombin, subunit H	Trypsin-like serine proteases	CATH (4.3.0)
B	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
C	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)

IMGT Antibody Annotation IMGT Database Homepage

Chain	Protein Name	Description	Organism Name	Gene Allele Name(s)	Domain Name(s)	Receptor Type	Receptor Description	Provenance Source
B	Fab S4	VH-CH1	Homo sapiens (human)	IGHV1-6901, IGHV1-6912, IGHV1-6913, IGHV1-69D01, IGHJ601, IGHJ602, IGHG101, IGHG102, IGHG104, IGHG105	V-DOMAIN VH, C-DOMAIN CH1	IG	FAB-GAMMA-1_V-LAMBDA-C-KAPPA	IMGT (202446-0)
C	Fab S4	V-LAMBDA-C-KAPPA	Homo sapiens (human)	IGLV1-4401, IGLV1-4701, IGLV1-4702, IGLJ302, IGKC*01	V-DOMAIN V-LAMBDA, C-DOMAIN C-KAPPA	IG	FAB-GAMMA-1_V-LAMBDA-C-KAPPA	IMGT (202446-0)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
B	Heavy Chain	IGHV1	-	suppressor of tumorigenicity 14 protein	SAbDab (2024-11-15)
C	Light Chain	IGLV1	Lambda	suppressor of tumorigenicity 14 protein	SAbDab (2024-11-15)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00089	Trypsin (Trypsin)	Trypsin	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Suppressor of tumorigenicity 14 protein	peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity serine-type endopeptidase activity catalytic activity endopeptidase activity	multicellular organismal process embryonic epithelial tube formation chordate embryonic development tube formation embryo development multicellular organism development epithelial tube formation tube morphogenesis primary neural tube formation embryo development ending in birth or egg hatching anatomical structure development epithelial tube morphogenesis system development developmental process multicellular organismal process embryonic epithelial tube formation chordate embryonic development tube formation embryo development multicellular organism development epithelial tube formation tube morphogenesis primary neural tube formation embryo development ending in birth or egg hatching anatomical structure development epithelial tube morphogenesis system development developmental process morphogenesis of embryonic epithelium epithelium development neural tube development tube development embryonic morphogenesis morphogenesis of an epithelium nervous system development tissue development anatomical structure morphogenesis tissue morphogenesis neural tube closure neural tube formation anatomical structure formation involved in morphogenesis tube closure epidermal cell differentiation cellular developmental process epidermis development animal organ development epithelial cell differentiation skin development cell differentiation keratinocyte differentiation cellular process catabolic process metabolic process primary metabolic process protein catabolic process protein metabolic process macromolecule metabolic process macromolecule catabolic process cell differentiation involved in embryonic placenta development epithelial cell differentiation involved in embryonic placenta development reproductive process cell development labyrinthine layer development developmental process involved in reproduction cell morphogenesis embryonic placenta development epithelial cell morphogenesis epithelial cell morphogenesis involved in placental branching embryonic organ development placenta development branching involved in labyrinthine layer morphogenesis in utero embryonic development embryonic placenta morphogenesis labyrinthine layer morphogenesis epithelial cell development morphogenesis of a branching structure morphogenesis of a branching epithelium proteolysis	cell periphery cellular anatomical structure cell surface membrane plasma membrane external side of plasma membrane side of membrane basal part of cell basal plasma membrane plasma membrane region basolateral plasma membrane extracellular region extracellular space
B	S4 FAB HEAVY CHAIN	-	-	-
C	S4 FAB LIGHT CHAIN	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR002172	Low-density lipoprotein (LDL) receptor class A repeat	Repeat
A	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
A	IPR017051	Peptidase S1A, matripase	Family
A	IPR033116	Serine proteases, trypsin family, serine active site	Active Site
A	IPR035914	Spermadhesin, CUB domain superfamily	Homologous Superfamily
A	IPR001254	Serine proteases, trypsin domain	Domain
A	IPR023415	Low-density lipoprotein (LDL) receptor class A, conserved site	Conserved Site
A	IPR036055	LDL receptor-like superfamily	Homologous Superfamily
A	IPR036364	SEA domain superfamily	Homologous Superfamily
A	IPR000859	CUB domain	Domain
A	IPR000082	SEA domain	Domain
A	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily
A	IPR018114	Serine proteases, trypsin family, histidine active site	Active Site

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: Q9Y5Y6	autosomal recessive congenital ichthyosis 11 Netherton syndrome Liver Cirrhosis, Experimental prostate cancer cystic fibrosis ichthyosis linearis circumflexa malignant pancreatic neoplasm osteosarcoma pancreatic intraductal papillary-mucinous neoplasm psoriasis atopic eczema ductal breast carcinoma in situ interstitial cystitis lung adenocarcinoma pancreatic ductal adenocarcinoma autosomal recessive congenital ichthyosis 11 Netherton syndrome Liver Cirrhosis, Experimental prostate cancer cystic fibrosis ichthyosis linearis circumflexa malignant pancreatic neoplasm osteosarcoma pancreatic intraductal papillary-mucinous neoplasm psoriasis atopic eczema ductal breast carcinoma in situ interstitial cystitis lung adenocarcinoma pancreatic ductal adenocarcinoma hypotrichosis invasive ductal breast carcinoma lung cancer non-small cell lung carcinoma pancreatic intraductal papillary-mucinous carcinoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.