6C0B

Structural basis for recognition of frizzled proteins by Clostridium difficile toxin B

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d6c0bb_	All alpha proteins	Frizzled cysteine-rich domain	Frizzled cysteine-rich domain	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2 Family	Frizzled cysteine-rich domain	8083833	4002966	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Frizzled domain-like	8083834	3001950	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	Fz	e6c0bB1	A: few secondary structure elements	X: Frizzled cysteine-rich domain-related (From Topology)	H: Frizzled cysteine-rich domain-related (From Topology)	T: Frizzled cysteine-rich domain-related	F: Fz	ECOD (1.6)
A	TcdA_TcdB_pore_2nd	e6c0bA1	A: beta duplicates or obligate multimers	X: Toxin A (TcdA) delivery domain (From Topology)	H: Toxin A (TcdA) delivery domain (From Topology)	T: Toxin A (TcdA) delivery domain	F: TcdA_TcdB_pore_2nd	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	1.10.2000.10	Mainly Alpha	Orthogonal Bundle	Frizzled cysteine-rich domain	Frizzled cysteine-rich domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B	PF01392	Fz domain (Fz)	Fz domain	Also known as the CRD (cysteine rich domain), the C6 box in MuSK receptor. This domain of unknown function has been independently identified by several groups [1,2,3,4]. The domain contains 10 conserved cysteines.	Domain
A	PF12920	TcdA/TcdB pore forming domain (TcdA_TcdB_pore)	TcdA/TcdB pore forming domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B	Frizzled-2	molecular transducer activity transmembrane signaling receptor activity signaling receptor activity Wnt receptor activity G protein-coupled receptor activity protein binding binding PDZ domain binding protein domain specific binding Wnt-protein binding	cellular response to stimulus regulation of biological process regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling canonical Wnt signaling pathway biological regulation cell communication cellular process signal transduction Wnt signaling pathway multicellular organismal process system process cellular response to stimulus regulation of biological process regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling canonical Wnt signaling pathway biological regulation cell communication cellular process signal transduction Wnt signaling pathway multicellular organismal process system process sensory perception of chemical stimulus sensory perception nervous system process sensory perception of smell non-canonical Wnt signaling pathway positive regulation of DNA-binding transcription factor activity RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process positive regulation of molecular function nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process primary metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of molecular function regulation of gene expression biosynthetic process regulation of DNA-binding transcription factor activity metabolic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription macromolecule metabolic process regulation of metabolic process developmental process heart development cardiac chamber morphogenesis animal organ development heart morphogenesis ventricular septum development circulatory system development anatomical structure morphogenesis multicellular organism development cardiac septum development cardiac septum morphogenesis ventricular septum morphogenesis anatomical structure development cardiac chamber development membranous septum morphogenesis animal organ morphogenesis cardiac ventricle development system development cell-cell signaling hard palate development secondary palate development roof of mouth development mechanoreceptor differentiation inner ear receptor cell development cellular developmental process ear development sensory organ development cell development nervous system development inner ear receptor cell differentiation generation of neurons neurogenesis neuron differentiation inner ear development cell differentiation neuron development muscle structure development muscle organ development tissue development muscle tissue morphogenesis tissue morphogenesis muscular septum morphogenesis muscle organ morphogenesis epithelium development endothelial cell differentiation endothelium development epithelial cell differentiation Wnt signaling pathway, planar cell polarity pathway ear morphogenesis embryonic organ morphogenesis embryonic morphogenesis embryo development sensory organ morphogenesis cochlea development inner ear morphogenesis cochlea morphogenesis embryonic organ development positive regulation of DNA-templated transcription positive regulation of biological process positive regulation of macromolecule biosynthetic process positive regulation of metabolic process positive regulation of biosynthetic process positive regulation of macromolecule metabolic process positive regulation of cellular process positive regulation of nucleobase-containing compound metabolic process positive regulation of RNA metabolic process positive regulation of RNA biosynthetic process outflow tract morphogenesis	cellular anatomical structure anchoring junction cell junction focal adhesion cell-substrate junction membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular organelle bounding membrane of organelle cytoplasmic vesicle vesicle membrane clathrin-coated vesicle endocytic vesicle membrane cellular anatomical structure anchoring junction cell junction focal adhesion cell-substrate junction membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular organelle bounding membrane of organelle cytoplasmic vesicle vesicle membrane clathrin-coated vesicle endocytic vesicle membrane coated vesicle vesicle endocytic vesicle clathrin-coated vesicle membrane clathrin-coated endocytic vesicle membrane membrane intracellular membrane-bounded organelle clathrin-coated endocytic vesicle cytoplasmic vesicle membrane coated vesicle membrane organelle cytoplasm organelle membrane cell periphery plasma membrane
A	Toxin B	binding lipid binding toxin activity glycosyltransferase activity transferase activity glucosyltransferase activity hexosyltransferase activity catalytic activity cysteine-type peptidase activity peptidase activity catalytic activity, acting on a protein hydrolase activity cation binding ion binding binding lipid binding toxin activity glycosyltransferase activity transferase activity glucosyltransferase activity hexosyltransferase activity catalytic activity cysteine-type peptidase activity peptidase activity catalytic activity, acting on a protein hydrolase activity cation binding ion binding metal ion binding small molecule binding	proteolysis metabolic process cellular process primary metabolic process protein metabolic process macromolecule metabolic process	cellular anatomical structure membrane extracellular region host intracellular membrane-bounded organelle host cellular component host intracellular part host cell membrane host cell cytoplasm part host intracellular organelle host cell endosome membrane host cell cytoplasm host cell part host cell endosome host intracellular region cellular anatomical structure membrane extracellular region host intracellular membrane-bounded organelle host cellular component host intracellular part host cell membrane host cell cytoplasm part host intracellular organelle host cell endosome membrane host cell cytoplasm host cell part host cell endosome host intracellular region host cell plasma membrane host cell cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B	IPR036790	Frizzled cysteine-rich domain superfamily	Homologous Superfamily
B	IPR041778	Frizzled 2, cysteine-rich domain	Domain
B	IPR017981	GPCR, family 2-like, 7TM	Domain
B	IPR015526	Frizzled/secreted frizzled-related protein	Family
B	IPR000539	Frizzled/Smoothened, 7TM	Domain
B	IPR020067	Frizzled domain	Domain
A	IPR038383	CGT/MARTX, cysteine protease (CPD) domain superfamily	Homologous Superfamily
A	IPR024772	TcdA/TcdB toxin, N-terminal helical domain	Domain
A	IPR020974	CGT/MARTX, cysteine protease (CPD) domain	Domain
A	IPR024769	TcdA/TcdB toxin, pore forming domain	Domain
A	IPR029044	Nucleotide-diphospho-sugar transferases	Homologous Superfamily
A	IPR024770	TcdA/TcdB toxin, catalytic glycosyltransferase domain	Domain
A	IPR018337	Cell wall/choline-binding repeat	Repeat

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B	Pharos: Q14332	malignant colon neoplasm Robinow syndrome atypical teratoid rhabdoid tumor breast cancer glioblastoma malignant glioma Omodysplasia 2 primitive neuroectodermal tumor tuberculosis autosomal dominant omodysplasia colonic neoplasm malignant mesothelioma ovarian cancer psoriasis benign colon neoplasm malignant colon neoplasm Robinow syndrome atypical teratoid rhabdoid tumor breast cancer glioblastoma malignant glioma Omodysplasia 2 primitive neuroectodermal tumor tuberculosis autosomal dominant omodysplasia colonic neoplasm malignant mesothelioma ovarian cancer psoriasis benign colon neoplasm gastric carcinoma malignant ependymoma medulloblastoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.