Annotations: 1DXE

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1dxea_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Phosphoenolpyruvate/pyruvate domain	HpcH/HpaI aldolase	2-dehydro-3-deoxy-galactarate aldolase	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d1dxeb_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Phosphoenolpyruvate/pyruvate domain	HpcH/HpaI aldolase	2-dehydro-3-deoxy-galactarate aldolase	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	HpcH/HpaI aldolase	8031279	4003274	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Phosphoenolpyruvate/pyruvate domain	8043657	3000510	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Phosphoenolpyruvate/pyruvate domain	8043657	3000510	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	HpcH_HpaI	e1dxeA1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: HpcH_HpaI	ECOD (1.6)
B	HpcH_HpaI	e1dxeB1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: HpcH_HpaI	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.60	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Phosphoenolpyruvate-binding domains	CATH (4.3.0)
B	3.20.20.60	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Phosphoenolpyruvate-binding domains	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF03328	HpcH/HpaI aldolase/citrate lyase family (HpcH_HpaI)	HpcH/HpaI aldolase/citrate lyase family	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	2-DEHYDRO-3-DEOXY-GALACTARATE ALDOLASE	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding lyase activity catalytic activity carbon-carbon lyase activity aldehyde-lyase activity 2-dehydro-3-deoxyglucarate aldolase activity	galactarate catabolic process galactarate metabolic process catabolic process oxoacid metabolic process cellular metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process organic acid metabolic process dicarboxylic acid catabolic process organic acid catabolic process aldaric acid metabolic process cellular catabolic process aldaric acid catabolic process carboxylic acid catabolic process galactarate catabolic process galactarate metabolic process catabolic process oxoacid metabolic process cellular metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process organic acid metabolic process dicarboxylic acid catabolic process organic acid catabolic process aldaric acid metabolic process cellular catabolic process aldaric acid catabolic process carboxylic acid catabolic process small molecule metabolic process metabolic process cellular process small molecule catabolic process D-glucarate catabolic process carbohydrate catabolic process carbohydrate metabolic process glucarate metabolic process primary metabolic process glucarate catabolic process D-glucarate metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR017648	5-keto-4-deoxy-D-glucarate aldolase	Family
A, B	IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	Homologous Superfamily
A, B	IPR005000	HpcH/HpaI aldolase/citrate lyase domain	Domain
A, B	IPR040442	Pyruvate kinase-like domain superfamily	Homologous Superfamily
A, B	IPR050251	HpcH/HpaI aldolase	Family

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	2-dehydro-3-deoxyglucarate aldolase M-CSA #846	5-keto-4-deoxy-D-glucarate aldolase GarL is involved in step 2 of the subpathway that synthesizes D-glycerate from galactarate. It catalyses the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde.	Defined by 5 residues: HIS:A-50ARG:A-75ASP:A_2-89GLU:A-153ASP:A-179 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 4.1.2.20 (PDB Primary Data) Search M-CSA Motif + EC 4.1.2.20

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.