1GOG

NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE

External Resource: Annotation


Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

ChainsDomain InfoClassFoldSuperfamilyFamilyDomainSpeciesProvenance Source (Version)
Ad1goga1 All beta proteins Immunoglobulin-like beta-sandwich E set domains E-set domains of sugar-utilizing enzymes Galactose oxidase, C-terminal domain (Hypomyces rosellus ) [TaxId: 5132 ], SCOPe (2.08)
Ad1goga2 All beta proteins Galactose-binding domain-like Galactose-binding domain-like Galactose-binding domain Galactose oxidase, N-terminal domain (Hypomyces rosellus ) [TaxId: 5132 ], SCOPe (2.08)
Ad1goga3 All beta proteins 7-bladed beta-propeller Galactose oxidase, central domain Galactose oxidase, central domain Galactose oxidase, central domain (Hypomyces rosellus ) [TaxId: 5132 ], SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

ChainsTypeFamily Name Domain Identifier Family IdentifierProvenance Source (Version)
ASCOP2B SuperfamilyGalactose oxidase, central domain 8042844 3001660 SCOP2B (2022-06-29)
ASCOP2B Superfamilygalactose-binding domain-like 8041133 3000087 SCOP2B (2022-06-29)
ASCOP2B SuperfamilyE set domains 8054887 3000070 SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

ChainsFamily NameDomain Identifier ArchitecturePossible HomologyHomologyTopologyFamilyProvenance Source (Version)
AF5_F8_type_C_1e1gogA1 A: beta sandwichesX: jelly-rollH: Concanavalin A-likeT: Galactose-binding domain-likeF: F5_F8_type_C_1ECOD (1.6)
ADUF1929e1gogA2 A: beta sandwichesX: Immunoglobulin-like beta-sandwichH: Immunoglobulin-relatedT: Immunoglobulin/Fibronectin type III/E set domains/PapD-likeF: DUF1929ECOD (1.6)
AGlyoxal_oxid_Ne1gogA3 A: beta duplicates or obligate multimersX: beta-propeller-likeH: beta-propellerT: 7-bladedF: Glyoxal_oxid_NECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

ChainDomainClassArchitectureTopologyHomologyProvenance Source (Version)
A2.60.120.260 Mainly Beta Sandwich Jelly Rolls Galactose-binding domain-likeCATH (4.3.0)
A2.130.10.80 Mainly Beta 7 Propeller Methylamine Dehydrogenase Chain HCATH (4.3.0)
A2.60.40.10 Mainly Beta Sandwich Immunoglobulin-like ImmunoglobulinsCATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

ChainsAccessionNameDescriptionCommentsSource
PF00754F5/8 type C domain (F5_F8_type_C)F5/8 type C domainThis domain is also known as the discoidin (DS) domain family [1].Domain
PF01344Kelch motif (Kelch_1)Kelch motif- Repeat
PF09118Galactose oxidase-like, Early set domain (GO-like_E_set)Galactose oxidase-like, Early set domainE or 'early' set domains are associated with the catalytic domain of galactose oxidase at the C-terminal end. Galactose oxidase is an extracellular monomeric enzyme which catalyzes the stereospecific oxidation of a broad range of primary alcohol subs ...E or 'early' set domains are associated with the catalytic domain of galactose oxidase at the C-terminal end. Galactose oxidase is an extracellular monomeric enzyme which catalyzes the stereospecific oxidation of a broad range of primary alcohol substrates, and possesses a unique mononuclear copper site essential for catalysing a two-electron transfer reaction during the oxidation of primary alcohols to corresponding aldehydes. The second redox active centre necessary for the reaction was found to be situated at a tyrosine residue. The C-terminal domain of galactose oxidase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end, and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family adopt a secondary structure consisting of a bundle of seven, mostly antiparallel, beta-strands surrounding a hydrophobic core. The 7 strands are arranged in 2 sheets, in a Greek-key topology [1]. This domain is found in sugar-utilising enzymes, such as galactose oxidase or chitinase [2-6].
Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

ChainsPolymerMolecular FunctionBiological ProcessCellular Component
GALACTOSE OXIDASE -

InterPro: Protein Family Classification InterPro Database Homepage

ChainsAccessionNameType
IPR000421Coagulation factor 5/8, C-terminal domainDomain
IPR013783Immunoglobulin-like foldHomologous Superfamily
IPR015202Galactose oxidase-like, Early set domainDomain
IPR014756Immunoglobulin E-setHomologous Superfamily
IPR037293Galactose oxidase, central domain superfamilyHomologous Superfamily
IPR006652Kelch repeat type 1Repeat
IPR011043Galactose oxidase/kelch, beta-propellerHomologous Superfamily
IPR008979Galactose-binding-like domain superfamilyHomologous Superfamily

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

ChainsEnzyme NameDescriptionCatalytic Residues
galactose oxidiase  M-CSA #322

Galactose oxidase is a copper containing enzyme which oxidises primary alcohols to aldehydes by the reduction of molecular oxygen to hydrogen peroxide. The enzyme catalyses the oxidation of many substrates, including dihydroxyacetone, small molecules and olysaccharides by use of a protein radical cofactor, a novel metalloradical complex, formed by a unique structural feature at the copper site with a novel thioether bond linking Cys 228 and Tyr 272 formed from a post-translational modification, which is in a stacking interaction with Trp 290. Galactose oxidase is remarkable in the extent to which free radicals are involved in all aspects of the enzyme function: serving as a key feature of the active site structure, defining the characteristic reactivity of the complex, and directing the biogenesis of the Tyr-Cys cofactor during protein maturation.

Defined by 6 residues: CYS:A-228TYR:A-272TRP:A-290TYR:A-495HIS:A-496HIS:A-581
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Explore in 3D: M-CSA Motif Definition
Search M-CSA Motif
EC: 1.1.3.9 (PDB Primary Data)