1M6V

Crystal Structure of the G359F (small subunit) Point Mutant of Carbamoyl Phosphate Synthetase

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1m6va1	All alpha proteins	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1m6va2	Alpha and beta proteins (a/b)	Methylglyoxal synthase-like	Methylglyoxal synthase-like	Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain	Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1m6va3	Alpha and beta proteins (a/b)	PreATP-grasp domain	PreATP-grasp domain	BC N-terminal domain-like	Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1m6va5	Alpha and beta proteins (a+b)	ATP-grasp	Glutathione synthetase ATP-binding domain-like	BC ATP-binding domain-like	Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d1m6vc1	All alpha proteins	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d1m6vc2	Alpha and beta proteins (a/b)	Methylglyoxal synthase-like	Methylglyoxal synthase-like	Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain	Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d1m6vc3	Alpha and beta proteins (a/b)	PreATP-grasp domain	PreATP-grasp domain	BC N-terminal domain-like	Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d1m6vc5	Alpha and beta proteins (a+b)	ATP-grasp	Glutathione synthetase ATP-binding domain-like	BC ATP-binding domain-like	Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
E	d1m6ve1	All alpha proteins	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
E	d1m6ve2	Alpha and beta proteins (a/b)	Methylglyoxal synthase-like	Methylglyoxal synthase-like	Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain	Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
E	d1m6ve3	Alpha and beta proteins (a/b)	PreATP-grasp domain	PreATP-grasp domain	BC N-terminal domain-like	Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
E	d1m6ve5	Alpha and beta proteins (a+b)	ATP-grasp	Glutathione synthetase ATP-binding domain-like	BC ATP-binding domain-like	Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
G	d1m6vg1	All alpha proteins	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	Carbamoyl phosphate synthetase, large subunit connection domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
G	d1m6vg2	Alpha and beta proteins (a/b)	Methylglyoxal synthase-like	Methylglyoxal synthase-like	Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain	Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
G	d1m6vg3	Alpha and beta proteins (a/b)	PreATP-grasp domain	PreATP-grasp domain	BC N-terminal domain-like	Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
G	d1m6vg5	Alpha and beta proteins (a+b)	ATP-grasp	Glutathione synthetase ATP-binding domain-like	BC ATP-binding domain-like	Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D	d1m6vd1	Alpha and beta proteins (a/b)	The 'swivelling' beta/beta/alpha domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D	d1m6vd2	Alpha and beta proteins (a/b)	Flavodoxin-like	Class I glutamine amidotransferase-like	Class I glutamine amidotransferases (GAT)	Carbamoyl phosphate synthetase, small subunit C-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
F	d1m6vf1	Alpha and beta proteins (a/b)	The 'swivelling' beta/beta/alpha domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
F	d1m6vf2	Alpha and beta proteins (a/b)	Flavodoxin-like	Class I glutamine amidotransferase-like	Class I glutamine amidotransferases (GAT)	Carbamoyl phosphate synthetase, small subunit C-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
H	d1m6vh1	Alpha and beta proteins (a/b)	The 'swivelling' beta/beta/alpha domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
H	d1m6vh2	Alpha and beta proteins (a/b)	Flavodoxin-like	Class I glutamine amidotransferase-like	Class I glutamine amidotransferases (GAT)	Carbamoyl phosphate synthetase, small subunit C-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d1m6vb1	Alpha and beta proteins (a/b)	The 'swivelling' beta/beta/alpha domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	Carbamoyl phosphate synthetase, small subunit N-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d1m6vb2	Alpha and beta proteins (a/b)	Flavodoxin-like	Class I glutamine amidotransferase-like	Class I glutamine amidotransferases (GAT)	Carbamoyl phosphate synthetase, small subunit C-terminal domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Glutathione synthetase ATP-binding domain-like	8036563	3000094	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	PreATP-grasp domain	8044230	3001680	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	PreATP-grasp domain	8044232	3001680	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Carbamoyl phosphate synthetase, large subunit connection domain	8038131	3000839	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Methylglyoxal synthase-like	8044174	3001443	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Glutathione synthetase ATP-binding domain-like	8036561	3000094	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Glutathione synthetase ATP-binding domain-like	8036563	3000094	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Carbamoyl phosphate synthetase, large subunit connection domain	8038131	3000839	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	PreATP-grasp domain	8044230	3001680	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	PreATP-grasp domain	8044232	3001680	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Methylglyoxal synthase-like	8044174	3001443	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Glutathione synthetase ATP-binding domain-like	8036561	3000094	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	PreATP-grasp domain	8044232	3001680	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	PreATP-grasp domain	8044230	3001680	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Carbamoyl phosphate synthetase, large subunit connection domain	8038131	3000839	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Glutathione synthetase ATP-binding domain-like	8036563	3000094	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Methylglyoxal synthase-like	8044174	3001443	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Glutathione synthetase ATP-binding domain-like	8036561	3000094	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Glutathione synthetase ATP-binding domain-like	8036561	3000094	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Glutathione synthetase ATP-binding domain-like	8036563	3000094	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Carbamoyl phosphate synthetase, large subunit connection domain	8038131	3000839	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Methylglyoxal synthase-like	8044174	3001443	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	PreATP-grasp domain	8044230	3001680	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	PreATP-grasp domain	8044232	3001680	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Carbamoyl phosphate synthetase, small subunit N-terminal domain	8043909	3000949	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8044144	3001405	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8044144	3001405	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Carbamoyl phosphate synthetase, small subunit N-terminal domain	8043909	3000949	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8044144	3001405	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Carbamoyl phosphate synthetase, small subunit N-terminal domain	8043909	3000949	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Carbamoyl phosphate synthetase, small subunit N-terminal domain	8043909	3000949	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8044144	3001405	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	CPSase_L_D3	e1m6vA1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: CPSase_L_D3	ECOD (1.6)
A	ATP-grasp	e1m6vA2	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: ATP-grasp	F: ATP-grasp	ECOD (1.6)
A	carB	e1m6vA4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: PreATP-grasp domain	F: carB	ECOD (1.6)
A	MGS	e1m6vA6	A: a/b three-layered sandwiches	X: Methylglyoxal synthase-like (From Topology)	H: Methylglyoxal synthase-like (From Topology)	T: Methylglyoxal synthase-like	F: MGS	ECOD (1.6)
C	CPSase_L_D3	e1m6vC1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: CPSase_L_D3	ECOD (1.6)
C	ATP-grasp	e1m6vC2	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: ATP-grasp	F: ATP-grasp	ECOD (1.6)
C	carB	e1m6vC4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: PreATP-grasp domain	F: carB	ECOD (1.6)
C	MGS	e1m6vC6	A: a/b three-layered sandwiches	X: Methylglyoxal synthase-like (From Topology)	H: Methylglyoxal synthase-like (From Topology)	T: Methylglyoxal synthase-like	F: MGS	ECOD (1.6)
E	CPSase_L_D3	e1m6vE1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: CPSase_L_D3	ECOD (1.6)
E	ATP-grasp	e1m6vE2	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: ATP-grasp	F: ATP-grasp	ECOD (1.6)
E	carB	e1m6vE4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: PreATP-grasp domain	F: carB	ECOD (1.6)
E	MGS	e1m6vE6	A: a/b three-layered sandwiches	X: Methylglyoxal synthase-like (From Topology)	H: Methylglyoxal synthase-like (From Topology)	T: Methylglyoxal synthase-like	F: MGS	ECOD (1.6)
G	CPSase_L_D3	e1m6vG1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: CPSase_L_D3	ECOD (1.6)
G	ATP-grasp	e1m6vG2	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: ATP-grasp	F: ATP-grasp	ECOD (1.6)
G	carB	e1m6vG4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: PreATP-grasp domain	F: carB	ECOD (1.6)
G	MGS	e1m6vG6	A: a/b three-layered sandwiches	X: Methylglyoxal synthase-like (From Topology)	H: Methylglyoxal synthase-like (From Topology)	T: Methylglyoxal synthase-like	F: MGS	ECOD (1.6)
D	GATase	e1m6vD1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase	ECOD (1.6)
D	CPSase_sm_chain	e1m6vD2	A: a/b three-layered sandwiches	X: The swivelling beta/beta/alpha domains	H: The swivelling beta/beta/alpha domain (From Topology)	T: The swivelling beta/beta/alpha domain	F: CPSase_sm_chain	ECOD (1.6)
F	GATase	e1m6vF1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase	ECOD (1.6)
F	CPSase_sm_chain	e1m6vF2	A: a/b three-layered sandwiches	X: The swivelling beta/beta/alpha domains	H: The swivelling beta/beta/alpha domain (From Topology)	T: The swivelling beta/beta/alpha domain	F: CPSase_sm_chain	ECOD (1.6)
H	GATase	e1m6vH1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase	ECOD (1.6)
H	CPSase_sm_chain	e1m6vH2	A: a/b three-layered sandwiches	X: The swivelling beta/beta/alpha domains	H: The swivelling beta/beta/alpha domain (From Topology)	T: The swivelling beta/beta/alpha domain	F: CPSase_sm_chain	ECOD (1.6)
B	GATase	e1m6vB1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase	ECOD (1.6)
B	CPSase_sm_chain	e1m6vB2	A: a/b three-layered sandwiches	X: The swivelling beta/beta/alpha domains	H: The swivelling beta/beta/alpha domain (From Topology)	T: The swivelling beta/beta/alpha domain	F: CPSase_sm_chain	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.20	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
A	3.30.470.20	Alpha Beta	2-Layer Sandwich	D-amino Acid Aminotransferase	Chain A, domain 1	CATH (4.3.0)
A	3.30.1490.20	Alpha Beta	2-Layer Sandwich	Dna Ligase	domain 1	CATH (4.3.0)
A	1.10.1030.10	Mainly Alpha	Orthogonal Bundle	Carbamoyl Phosphate Synthetase	Chain A, domain 4	CATH (4.3.0)
A	3.40.50.1380	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Methylglyoxal synthase-like domain	CATH (4.3.0)
C	3.40.50.20	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
C	3.30.470.20	Alpha Beta	2-Layer Sandwich	D-amino Acid Aminotransferase	Chain A, domain 1	CATH (4.3.0)
C	3.30.1490.20	Alpha Beta	2-Layer Sandwich	Dna Ligase	domain 1	CATH (4.3.0)
C	1.10.1030.10	Mainly Alpha	Orthogonal Bundle	Carbamoyl Phosphate Synthetase	Chain A, domain 4	CATH (4.3.0)
C	3.40.50.1380	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Methylglyoxal synthase-like domain	CATH (4.3.0)
E	3.40.50.20	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
E	3.30.470.20	Alpha Beta	2-Layer Sandwich	D-amino Acid Aminotransferase	Chain A, domain 1	CATH (4.3.0)
E	3.30.1490.20	Alpha Beta	2-Layer Sandwich	Dna Ligase	domain 1	CATH (4.3.0)
E	1.10.1030.10	Mainly Alpha	Orthogonal Bundle	Carbamoyl Phosphate Synthetase	Chain A, domain 4	CATH (4.3.0)
E	3.40.50.1380	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Methylglyoxal synthase-like domain	CATH (4.3.0)
G	3.40.50.20	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
G	3.30.470.20	Alpha Beta	2-Layer Sandwich	D-amino Acid Aminotransferase	Chain A, domain 1	CATH (4.3.0)
G	3.30.1490.20	Alpha Beta	2-Layer Sandwich	Dna Ligase	domain 1	CATH (4.3.0)
G	1.10.1030.10	Mainly Alpha	Orthogonal Bundle	Carbamoyl Phosphate Synthetase	Chain A, domain 4	CATH (4.3.0)
G	3.40.50.1380	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Methylglyoxal synthase-like domain	CATH (4.3.0)
D	3.50.30.20	Alpha Beta	3-Layer(bba) Sandwich	Glucose Oxidase	domain 1	CATH (4.3.0)
D	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
F	3.50.30.20	Alpha Beta	3-Layer(bba) Sandwich	Glucose Oxidase	domain 1	CATH (4.3.0)
F	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
H	3.50.30.20	Alpha Beta	3-Layer(bba) Sandwich	Glucose Oxidase	domain 1	CATH (4.3.0)
H	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
B	3.50.30.20	Alpha Beta	3-Layer(bba) Sandwich	Glucose Oxidase	domain 1	CATH (4.3.0)
B	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C, E, G	PF02142	MGS-like domain (MGS)	MGS-like domain	This domain composes the whole protein of methylglyoxal synthetase and the domain is also found in Carbamoyl phosphate synthetase (CPS) where it forms a regulatory domain that binds to the allosteric effector ornithine. This family also includes ino ...	This domain composes the whole protein of methylglyoxal synthetase and the domain is also found in Carbamoyl phosphate synthetase (CPS) where it forms a regulatory domain that binds to the allosteric effector ornithine. This family also includes inosicase. The known structures in this family show a common phosphate binding site [1].	Domain
A, C, E, G	PF02786	Carbamoyl-phosphate synthase L chain, ATP binding domain (CPSase_L_D2)	Carbamoyl-phosphate synthase L chain, ATP binding domain	Carbamoyl-phosphate synthase catalyses the ATP-dependent synthesis of carbamyl-phosphate from glutamine or ammonia and bicarbonate. This important enzyme initiates both the urea cycle and the biosynthesis of arginine and/or pyrimidines [2]. The c ...	Carbamoyl-phosphate synthase catalyses the ATP-dependent synthesis of carbamyl-phosphate from glutamine or ammonia and bicarbonate. This important enzyme initiates both the urea cycle and the biosynthesis of arginine and/or pyrimidines [2]. The carbamoyl-phosphate synthase (CPS) enzyme in prokaryotes is a heterodimer of a small and large chain. The small chain promotes the hydrolysis of glutamine to ammonia, which is used by the large chain to synthesise carbamoyl phosphate. See Pfam:PF00988. The small chain has a GATase domain in the carboxyl terminus. See Pfam:PF00117. The ATP binding domain (this one) has an ATP-grasp fold.	Domain
A, C, E, G	PF02787	Carbamoyl-phosphate synthetase large chain, oligomerisation domain (CPSase_L_D3)	Carbamoyl-phosphate synthetase large chain, oligomerisation domain	Carbamoyl-phosphate synthase catalyses the ATP-dependent synthesis of carbamyl-phosphate from glutamine or ammonia and bicarbonate. The carbamoyl-phosphate synthase (CPS) enzyme in prokaryotes is a heterodimer of a small and large chain.	Domain
B, D, F, H	PF00117	Glutamine amidotransferase class-I (GATase)	Glutamine amidotransferase class-I	-	Domain
B, D, F, H	PF00988	Carbamoyl-phosphate synthase small chain, CPSase domain (CPSase_sm_chain)	Carbamoyl-phosphate synthase small chain, CPSase domain	The carbamoyl-phosphate synthase domain is in the amino terminus of protein. Carbamoyl-phosphate synthase catalyses the ATP-dependent synthesis of carbamyl-phosphate from glutamine or ammonia and bicarbonate. This important enzyme initiates both ...	The carbamoyl-phosphate synthase domain is in the amino terminus of protein. Carbamoyl-phosphate synthase catalyses the ATP-dependent synthesis of carbamyl-phosphate from glutamine or ammonia and bicarbonate. This important enzyme initiates both the urea cycle and the biosynthesis of arginine and/or pyrimidines [1]. The carbamoyl-phosphate synthase (CPS) enzyme in prokaryotes is a heterodimer of a small and large chain. The small chain promotes the hydrolysis of glutamine to ammonia, which is used by the large chain to synthesise carbamoyl phosphate. See Pfam:PF00289. The small chain has a GATase domain in the carboxyl terminus. See Pfam:PF00117.	Domain
B, D, F, H	PF07722	Peptidase C26 (Peptidase_C26)	Peptidase C26	These peptidases have gamma-glutamyl hydrolase activity; that is they catalyse the cleavage of the gamma-glutamyl bond in poly-gamma-glutamyl substrates. They are structurally related to Pfam:PF00117, but contain extensions in four loops and at the C ...	These peptidases have gamma-glutamyl hydrolase activity; that is they catalyse the cleavage of the gamma-glutamyl bond in poly-gamma-glutamyl substrates. They are structurally related to Pfam:PF00117, but contain extensions in four loops and at the C terminus [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C, E, G	carbamoyl phosphate synthetase large chain	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding amino acid binding cation binding metal ion binding catalytic activity carbamoyl-phosphate synthase (ammonia) activity ligase activity, forming carbon-nitrogen bonds ligase activity carbon-nitrogen ligase activity, with glutamine as amido-N-donor carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	pyrimidine-containing compound metabolic process nucleobase biosynthetic process pyrimidine nucleobase biosynthetic process primary metabolic process nucleobase-containing compound metabolic process pyrimidine nucleobase metabolic process biosynthetic process nucleobase-containing small molecule metabolic process nucleobase metabolic process small molecule metabolic process metabolic process pyrimidine-containing compound biosynthetic process amino acid biosynthetic process amino acid metabolic process pyrimidine-containing compound metabolic process nucleobase biosynthetic process pyrimidine nucleobase biosynthetic process primary metabolic process nucleobase-containing compound metabolic process pyrimidine nucleobase metabolic process biosynthetic process nucleobase-containing small molecule metabolic process nucleobase metabolic process small molecule metabolic process metabolic process pyrimidine-containing compound biosynthetic process amino acid biosynthetic process amino acid metabolic process L-arginine biosynthetic process oxoacid metabolic process glutamine family amino acid biosynthetic process cellular biosynthetic process organic acid metabolic process organic acid biosynthetic process alpha-amino acid metabolic process carboxylic acid biosynthetic process glutamine family amino acid metabolic process arginine metabolic process small molecule biosynthetic process cellular metabolic process carboxylic acid metabolic process L-amino acid biosynthetic process L-amino acid metabolic process proteinogenic amino acid metabolic process proteinogenic amino acid biosynthetic process cellular process alpha-amino acid biosynthetic process glutamine metabolic process ribose phosphate metabolic process nucleotide biosynthetic process carbohydrate derivative metabolic process pyrimidine nucleotide metabolic process 'de novo' UMP biosynthetic process ribonucleotide biosynthetic process UMP biosynthetic process ribonucleotide metabolic process ribonucleoside monophosphate metabolic process phosphate-containing compound metabolic process UMP metabolic process phosphorus metabolic process nucleoside monophosphate metabolic process ribose phosphate biosynthetic process pyrimidine ribonucleotide metabolic process nucleoside phosphate metabolic process carbohydrate derivative biosynthetic process pyrimidine nucleoside monophosphate metabolic process pyrimidine ribonucleoside monophosphate metabolic process nucleobase-containing compound biosynthetic process nucleotide metabolic process nucleoside monophosphate biosynthetic process pyrimidine ribonucleoside monophosphate biosynthetic process organophosphate metabolic process organophosphate biosynthetic process nucleoside phosphate biosynthetic process pyrimidine nucleoside monophosphate biosynthetic process ribonucleoside monophosphate biosynthetic process pyrimidine nucleotide biosynthetic process pyrimidine ribonucleotide biosynthetic process	protein-containing complex intracellular anatomical structure catalytic complex carbamoyl-phosphate synthase complex cytoplasm cellular anatomical entity cytosol
B, D, F, H	carbamoyl-phosphate synthetase small chain	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides glutaminase activity hydrolase activity catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds protein heterodimerization activity protein binding protein dimerization activity carbon-nitrogen ligase activity, with glutamine as amido-N-donor ligase activity, forming carbon-nitrogen bonds carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity ligase activity	pyrimidine-containing compound metabolic process nucleobase biosynthetic process pyrimidine nucleobase biosynthetic process primary metabolic process nucleobase-containing compound metabolic process pyrimidine nucleobase metabolic process biosynthetic process nucleobase-containing small molecule metabolic process nucleobase metabolic process small molecule metabolic process metabolic process pyrimidine-containing compound biosynthetic process L-arginine biosynthetic process oxoacid metabolic process pyrimidine-containing compound metabolic process nucleobase biosynthetic process pyrimidine nucleobase biosynthetic process primary metabolic process nucleobase-containing compound metabolic process pyrimidine nucleobase metabolic process biosynthetic process nucleobase-containing small molecule metabolic process nucleobase metabolic process small molecule metabolic process metabolic process pyrimidine-containing compound biosynthetic process L-arginine biosynthetic process oxoacid metabolic process glutamine family amino acid biosynthetic process cellular biosynthetic process amino acid metabolic process organic acid metabolic process organic acid biosynthetic process alpha-amino acid metabolic process carboxylic acid biosynthetic process glutamine family amino acid metabolic process arginine metabolic process small molecule biosynthetic process cellular metabolic process carboxylic acid metabolic process L-amino acid biosynthetic process L-amino acid metabolic process proteinogenic amino acid metabolic process amino acid biosynthetic process proteinogenic amino acid biosynthetic process cellular process alpha-amino acid biosynthetic process 'de novo' pyrimidine nucleobase biosynthetic process glutamine metabolic process ribose phosphate metabolic process nucleotide biosynthetic process carbohydrate derivative metabolic process pyrimidine nucleotide metabolic process 'de novo' UMP biosynthetic process ribonucleotide biosynthetic process UMP biosynthetic process ribonucleotide metabolic process ribonucleoside monophosphate metabolic process phosphate-containing compound metabolic process UMP metabolic process phosphorus metabolic process nucleoside monophosphate metabolic process ribose phosphate biosynthetic process pyrimidine ribonucleotide metabolic process nucleoside phosphate metabolic process carbohydrate derivative biosynthetic process pyrimidine nucleoside monophosphate metabolic process pyrimidine ribonucleoside monophosphate metabolic process nucleobase-containing compound biosynthetic process nucleotide metabolic process nucleoside monophosphate biosynthetic process pyrimidine ribonucleoside monophosphate biosynthetic process organophosphate metabolic process organophosphate biosynthetic process nucleoside phosphate biosynthetic process pyrimidine nucleoside monophosphate biosynthetic process ribonucleoside monophosphate biosynthetic process pyrimidine nucleotide biosynthetic process pyrimidine ribonucleotide biosynthetic process	protein-containing complex intracellular anatomical structure catalytic complex carbamoyl-phosphate synthase complex cytoplasm cellular anatomical entity cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C, E, G	IPR005480	Carbamoyl-phosphate synthetase, large subunit oligomerisation domain	Domain
A, C, E, G	IPR011761	ATP-grasp fold	Domain
A, C, E, G	IPR036897	Carbamoyl-phosphate synthetase, large subunit oligomerisation domain superfamily	Homologous Superfamily
A, C, E, G	IPR033937	Carbamoyl-phosphate synthase large chain, methylglyoxal synthase-like domain	Domain
A, C, E, G	IPR011607	Methylglyoxal synthase-like domain	Domain
A, C, E, G	IPR036914	Methylglyoxal synthase-like domain superfamily	Homologous Superfamily
A, C, E, G	IPR006275	Carbamoyl-phosphate synthase, large subunit	Family
A, C, E, G	IPR005479	Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain	Domain
A, C, E, G	IPR016185	Pre-ATP-grasp domain superfamily	Homologous Superfamily
A, C, E, G	IPR005483	Carbamoyl-phosphate synthase large subunit, CPSase domain	Domain
B, D, F, H	IPR029062	Class I glutamine amidotransferase-like	Homologous Superfamily
B, D, F, H	IPR006274	Carbamoyl-phosphate synthase, small subunit	Family
B, D, F, H	IPR002474	Carbamoyl-phosphate synthase small subunit, N-terminal domain	Domain
B, D, F, H	IPR017926	Glutamine amidotransferase	Domain
B, D, F, H	IPR035686	Carbamoyl-phosphate synthase small subunit, GATase1 domain	Domain
B, D, F, H	IPR036480	Carbamoyl-phosphate synthase small subunit, N-terminal domain superfamily	Homologous Superfamily