1N4W

ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ pH 7.3 (STREPTOMYCES SP. SA-COO)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1n4wa1	Alpha and beta proteins (a/b)	FAD/NAD(P)-binding domain	FAD/NAD(P)-binding domain	FAD-linked reductases, N-terminal domain	Cholesterol oxidase of GMC family	(Streptomyces sp. SA-COO ) [TaxId: 74576 ],	SCOPe (2.08)
A	d1n4wa2	Alpha and beta proteins (a+b)	FAD-linked reductases, C-terminal domain	FAD-linked reductases, C-terminal domain	GMC oxidoreductases	Cholesterol oxidase	(Streptomyces sp. SA-COO ) [TaxId: 74576 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Flavoreductase-like	8058150	3000055	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	EUF08299	e1n4wA1	A: a+b two layers	X: FAD-linked reductases, C-terminal domain-like	H: FAD-linked reductases-C (From Topology)	T: FAD-linked reductases-C	F: EUF08299	ECOD (1.6)
A	GMC_oxred_N	e1n4wA2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: FAD/NAD(P)-binding domain	F: GMC_oxred_N	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.50.50.60	Alpha Beta	3-Layer(bba) Sandwich	FAD/NAD(P)-binding domain	FAD/NAD(P)-binding domain	CATH (4.3.0)
A	3.30.410.10	Alpha Beta	2-Layer Sandwich	Cholesterol Oxidase	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF22500	GMC oxidoreductase, C-terminal (GMC_oxred_C_1st)	GMC oxidoreductase, C-terminal	This domain is found in a group of GMC oxidoreductases mainly from actinomycetes, including Cholesterol oxidase from Streptomyces sp. (ChoA). ChoA is organised into two domains, an FAD-binding domain (Pfam:PF00732) and a substrate binding domain (for ...	This domain is found in a group of GMC oxidoreductases mainly from actinomycetes, including Cholesterol oxidase from Streptomyces sp. (ChoA). ChoA is organised into two domains, an FAD-binding domain (Pfam:PF00732) and a substrate binding domain (formed by this entry and Pfam:PF05199) [1-5]. This domain consists of six beta -sheets and one alpha-helix.	Domain
A	PF05199	GMC oxidoreductase (GMC_oxred_C)	GMC oxidoreductase	This domain found associated with Pfam:PF00732.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Cholesterol oxidase	oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor cholesterol oxidase activity oxidoreductase activity oxidoreductase activity, acting on CH-OH group of donors catalytic activity isomerase activity intramolecular oxidoreductase activity, transposing C=C bonds steroid delta-isomerase activity intramolecular oxidoreductase activity flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor cholesterol oxidase activity oxidoreductase activity oxidoreductase activity, acting on CH-OH group of donors catalytic activity isomerase activity intramolecular oxidoreductase activity, transposing C=C bonds steroid delta-isomerase activity intramolecular oxidoreductase activity flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding	sterol catabolic process alcohol catabolic process catabolic process lipid catabolic process organic hydroxy compound metabolic process sterol metabolic process steroid metabolic process cholesterol metabolic process alcohol metabolic process primary metabolic process cholesterol catabolic process secondary alcohol metabolic process steroid catabolic process organic hydroxy compound catabolic process sterol catabolic process alcohol catabolic process catabolic process lipid catabolic process organic hydroxy compound metabolic process sterol metabolic process steroid metabolic process cholesterol metabolic process alcohol metabolic process primary metabolic process cholesterol catabolic process secondary alcohol metabolic process steroid catabolic process organic hydroxy compound catabolic process metabolic process small molecule metabolic process small molecule catabolic process lipid metabolic process	extracellular region cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	Domain
A	IPR052542	Cholesterol Oxidase Enzyme	Family
A	IPR006311	Twin-arginine translocation pathway, signal sequence	Conserved Site
A	IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	Domain
A	IPR036188	FAD/NAD(P)-binding domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.