1O0S

Crystal Structure of Ascaris suum Malic Enzyme Complexed with NADH

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1o0sa1	Alpha and beta proteins (a/b)	NAD(P)-binding Rossmann-fold domains	NAD(P)-binding Rossmann-fold domains	Aminoacid dehydrogenase-like, C-terminal domain	Mitochondrial NAD(P)-dependent malic enzyme	(Ascaris suum ) [TaxId: 6253 ],	SCOPe (2.08)
A	d1o0sa2	Alpha and beta proteins (a/b)	Aminoacid dehydrogenase-like, N-terminal domain	Aminoacid dehydrogenase-like, N-terminal domain	Malic enzyme N-domain	Mitochondrial NAD(P)-dependent malic enzyme	(Ascaris suum ) [TaxId: 6253 ],	SCOPe (2.08)
B	d1o0sb1	Alpha and beta proteins (a/b)	NAD(P)-binding Rossmann-fold domains	NAD(P)-binding Rossmann-fold domains	Aminoacid dehydrogenase-like, C-terminal domain	Mitochondrial NAD(P)-dependent malic enzyme	(Ascaris suum ) [TaxId: 6253 ],	SCOPe (2.08)
B	d1o0sb2	Alpha and beta proteins (a/b)	Aminoacid dehydrogenase-like, N-terminal domain	Aminoacid dehydrogenase-like, N-terminal domain	Malic enzyme N-domain	Mitochondrial NAD(P)-dependent malic enzyme	(Ascaris suum ) [TaxId: 6253 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Malate oxidoreductase-like	8056184	4000103	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	MtdA-like	8056185	3000046	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	MtdA-like	8056185	3000046	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Malic_M	e1o0sA3	A: alpha arrays	X: C-terminal domain of Malic enzyme (From Topology)	H: C-terminal domain of Malic enzyme (From Topology)	T: C-terminal domain of Malic enzyme	F: Malic_M	ECOD (1.6)
A	Malic_M	e1o0sA1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: Malic_M	ECOD (1.6)
A	malic	e1o0sA2	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Aminoacid dehydrogenase-like, N-terminal domain	F: malic	ECOD (1.6)
B	Malic_M	e1o0sB2	A: alpha arrays	X: C-terminal domain of Malic enzyme (From Topology)	H: C-terminal domain of Malic enzyme (From Topology)	T: C-terminal domain of Malic enzyme	F: Malic_M	ECOD (1.6)
B	Malic_M	e1o0sB3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: Malic_M	ECOD (1.6)
B	malic	e1o0sB1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Aminoacid dehydrogenase-like, N-terminal domain	F: malic	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	6.20.310.10	Special	Other non-globular	arginine biosynthesis bifunctional protein fold		CATH (4.3.0)
A	1.20.1370.30	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain		CATH (4.3.0)
A	3.40.50.10380	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Malic enzyme, N-terminal domain	CATH (4.3.0)
A	3.40.50.720	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	NAD(P)-binding Rossmann-like Domain	CATH (4.3.0)
B	6.20.310.10	Special	Other non-globular	arginine biosynthesis bifunctional protein fold		CATH (4.3.0)
B	1.20.1370.30	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain		CATH (4.3.0)
B	3.40.50.10380	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Malic enzyme, N-terminal domain	CATH (4.3.0)
B	3.40.50.720	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	NAD(P)-binding Rossmann-like Domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00390	Malic enzyme, N-terminal domain (malic)	Malic enzyme, N-terminal domain	-	Domain
A, B	PF03949	Malic enzyme, NAD binding domain (Malic_M)	Malic enzyme, NAD binding domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	NAD-dependent malic enzyme	oxidoreductase activity malate dehydrogenase (decarboxylating) (NADP+) activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors malic enzyme activity malate dehydrogenase activity catalytic activity malate dehydrogenase (decarboxylating) (NAD+) activity oxaloacetate decarboxylase activity carboxy-lyase activity lyase activity carbon-carbon lyase activity NAD binding binding oxidoreductase activity malate dehydrogenase (decarboxylating) (NADP+) activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors malic enzyme activity malate dehydrogenase activity catalytic activity malate dehydrogenase (decarboxylating) (NAD+) activity oxaloacetate decarboxylase activity carboxy-lyase activity lyase activity carbon-carbon lyase activity NAD binding binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding adenyl nucleotide binding nucleotide binding heterocyclic compound binding small molecule binding cation binding ion binding metal ion binding	oxoacid metabolic process malate metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process small molecule metabolic process metabolic process cellular process organic acid metabolic process pyruvate metabolic process monocarboxylic acid metabolic process	membrane-bounded organelle intracellular anatomical structure mitochondrion membrane-enclosed lumen intracellular membrane-bounded organelle mitochondrial matrix intracellular organelle lumen intracellular organelle cellular anatomical structure organelle lumen organelle cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR012302	Malic enzyme, NAD-binding	Domain
A, B	IPR012301	Malic enzyme, N-terminal domain	Domain
A, B	IPR046346	Aminoacid dehydrogenase-like, N-terminal domain superfamily	Homologous Superfamily
A, B	IPR015884	Malic enzyme, conserved site	Conserved Site
A, B	IPR036291	NAD(P)-binding domain superfamily	Homologous Superfamily
A, B	IPR037062	Malic enzyme, N-terminal domain superfamily	Homologous Superfamily
A, B	IPR001891	Malic oxidoreductase	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.