1QE3

PNB ESTERASE

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1qe3a_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Acetylcholinesterase-like	Thermophilic para-nitrobenzyl esterase (PNB esterase)	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	alpha/beta-Hydrolases	8032492	3000102	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	COesterase	e1qe3A1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: COesterase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00135	Carboxylesterase family (COesterase)	Carboxylesterase family	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	PARA-NITROBENZYL ESTERASE	hydrolase activity, acting on ester bonds cholinesterase activity carboxylic ester hydrolase activity hydrolase activity catalytic activity	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR019819	Carboxylesterase type B, conserved site	Conserved Site
A	IPR019826	Carboxylesterase type B, active site	Active Site
A	IPR000997	Cholinesterase	Family
A	IPR050309	Type-B Carboxylesterase/Lipase	Family
A	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily
A	IPR002018	Carboxylesterase, type B	Domain

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	para-nitrobenzyl esterase M-CSA #900	Catalyses hydrolysis of several beta-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol.	Defined by 6 residues: GLY:A-106ALA:A-107SER:A-189ALA:A-190GLU:A-310HIS:A-399 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.1.1 (PDB Primary Data) Search M-CSA Motif + EC 3.1.1

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.