2ERF

Crystal Structure of the Thrombospondin-1 N-terminal Domain at 1.45A Resolution

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2erfa_	All beta proteins	Concanavalin A-like lectins/glucanases	Concanavalin A-like lectins/glucanases	Laminin G-like module	Thrombospondin 1 N-terminal domain	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Laminin G-like module	8027745	4002689	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Concanavalin A-like lectins/glucanases	8040124	3000120	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Laminin_G_1	e2erfA1	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Concanavalin A-like lectins/glucanases	F: Laminin_G_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.120.200	Mainly Beta	Sandwich	Jelly Rolls		CATH (4.3.0)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Thrombospondin-1	protein-containing complex binding fibrinogen binding binding cation binding ion binding calcium ion binding metal ion binding small molecule binding protein binding enzyme binding protease binding transforming growth factor beta binding growth factor binding cytokine binding protein-containing complex binding fibrinogen binding binding cation binding ion binding calcium ion binding metal ion binding small molecule binding protein binding enzyme binding protease binding transforming growth factor beta binding growth factor binding cytokine binding structural molecule activity extracellular matrix structural constituent identical protein binding protein homodimerization activity protein dimerization activity lipoprotein particle binding protein-lipid complex binding low-density lipoprotein particle binding proteoglycan binding carbohydrate derivative binding enzyme regulator activity molecular function inhibitor activity peptidase regulator activity molecular function regulator activity enzyme inhibitor activity peptidase inhibitor activity endopeptidase regulator activity endopeptidase inhibitor activity extracellular matrix binding laminin binding fibroblast growth factor binding fibronectin binding modified amino acid binding phospholipid binding lipid binding phosphatidylserine binding collagen binding collagen V binding cell adhesion molecule binding integrin binding signaling receptor binding heparin binding glycosaminoglycan binding sulfur compound binding	response to stimulus response to chemical response to xenobiotic stimulus response to ketone response to lipid response to oxygen-containing compound response to testosterone response to organic cyclic compound response to topologically incorrect protein response to stress response to unfolded protein cellular response to stimulus cellular response to heat cellular response to stress response to stimulus response to chemical response to xenobiotic stimulus response to ketone response to lipid response to oxygen-containing compound response to testosterone response to organic cyclic compound response to topologically incorrect protein response to stress response to unfolded protein cellular response to stimulus cellular response to heat cellular response to stress response to heat response to abiotic stimulus cellular process response to temperature stimulus chemotaxis positive regulation of locomotion regulation of biological process regulation of response to external stimulus biological regulation positive regulation of biological process response to external stimulus regulation of chemotaxis positive regulation of chemotaxis positive regulation of response to stimulus positive regulation of response to external stimulus taxis regulation of response to stimulus regulation of locomotion locomotion apoptotic process cell death programmed cell death regulation of cellular process signaling negative regulation of signaling negative regulation of intracellular signal transduction negative regulation of biological process regulation of signal transduction intracellular signal transduction negative regulation of cell communication cell communication regulation of intracellular signal transduction intracellular signaling cassette negative regulation of cellular process signal transduction regulation of cGMP-mediated signaling regulation of signaling negative regulation of cGMP-mediated signaling negative regulation of response to stimulus cGMP-mediated signaling negative regulation of signal transduction regulation of cell communication behavior multicellular organismal process multicellular organismal response to stress response to pain behavioral response to pain regulation of cell motility macrophage migration positive regulation of cell migration cell migration regulation of immune system process regulation of mononuclear cell migration positive regulation of immune system process regulation of leukocyte chemotaxis positive regulation of macrophage migration regulation of leukocyte migration positive regulation of leukocyte chemotaxis granulocyte migration leukocyte migration macrophage chemotaxis regulation of granulocyte chemotaxis regulation of macrophage migration positive regulation of cell motility regulation of cell migration myeloid leukocyte migration positive regulation of mononuclear cell migration cell motility positive regulation of cellular process cell chemotaxis regulation of macrophage chemotaxis positive regulation of leukocyte migration cellular response to chemical stimulus immune system process granulocyte chemotaxis mononuclear cell migration positive regulation of macrophage chemotaxis leukocyte chemotaxis nitric oxide mediated signal transduction regulation of nitric oxide mediated signal transduction negative regulation of nitric oxide mediated signal transduction cell population proliferation regulation of smooth muscle cell proliferation positive regulation of cell population proliferation smooth muscle cell proliferation positive regulation of smooth muscle cell proliferation regulation of cell population proliferation muscle cell proliferation negative regulation of locomotion cell migration involved in sprouting angiogenesis regulation of blood vessel endothelial cell migration endothelial cell migration angiogenesis negative regulation of endothelial cell migration negative regulation of blood vessel endothelial cell migration multicellular organism development tube morphogenesis anatomical structure development blood vessel endothelial cell migration regulation of endothelial cell migration negative regulation of cell migration blood vessel development system development developmental process blood vessel morphogenesis tube development regulation of cell migration involved in sprouting angiogenesis sprouting angiogenesis vasculature development circulatory system development anatomical structure morphogenesis anatomical structure formation involved in morphogenesis negative regulation of cell motility negative regulation of cell migration involved in sprouting angiogenesis regulation of body fluid levels positive regulation of coagulation positive regulation of blood coagulation regulation of response to wounding regulation of coagulation regulation of response to stress coagulation regulation of multicellular organismal process positive regulation of hemostasis regulation of biological quality response to wounding regulation of hemostasis positive regulation of response to wounding wound healing regulation of blood coagulation hemostasis blood coagulation positive regulation of wound healing regulation of wound healing positive regulation of multicellular organismal process regulation of programmed cell death extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway negative regulation of apoptotic signaling pathway cell surface receptor signaling pathway negative regulation of programmed cell death regulation of apoptotic process regulation of extrinsic apoptotic signaling pathway apoptotic signaling pathway negative regulation of apoptotic process regulation of apoptotic signaling pathway response to endoplasmic reticulum stress response to fibroblast growth factor cellular response to growth factor stimulus negative regulation of cellular response to growth factor stimulus cell surface receptor protein tyrosine kinase signaling pathway cellular response to endogenous stimulus enzyme-linked receptor protein signaling pathway negative regulation of fibroblast growth factor receptor signaling pathway response to endogenous stimulus regulation of cellular response to growth factor stimulus fibroblast growth factor receptor signaling pathway response to growth factor cellular response to fibroblast growth factor stimulus regulation of fibroblast growth factor receptor signaling pathway regulation of macrophage activation positive regulation of macrophage activation regulation of leukocyte activation cell activation regulation of cell activation myeloid leukocyte activation leukocyte activation macrophage activation positive regulation of cell activation positive regulation of leukocyte activation chronic inflammatory response defense response inflammatory response regulation of sprouting angiogenesis regulation of developmental process regulation of vasculature development regulation of anatomical structure morphogenesis regulation of multicellular organismal development negative regulation of blood vessel morphogenesis negative regulation of developmental process negative regulation of angiogenesis negative regulation of vasculature development regulation of angiogenesis negative regulation of multicellular organismal process negative regulation of sprouting angiogenesis immune response response to hexose response to monosaccharide response to carbohydrate response to glucose cellular metabolic process reactive oxygen species metabolic process regulation of cellular metabolic process positive regulation of cellular metabolic process positive regulation of reactive oxygen species metabolic process positive regulation of metabolic process metabolic process regulation of metabolic process regulation of reactive oxygen species metabolic process response to peptide cellular response to tumor necrosis factor response to cytokine cellular response to cytokine stimulus response to tumor necrosis factor positive regulation of blood vessel endothelial cell migration positive regulation of endothelial cell migration positive regulation of cell communication positive regulation of signal transduction positive regulation of phosphorylation regulation of catalytic activity regulation of transferase activity positive regulation of phosphate metabolic process regulation of MAP kinase activity regulation of primary metabolic process positive regulation of protein serine/threonine kinase activity regulation of phosphate metabolic process phosphate-containing compound metabolic process positive regulation of macromolecule metabolic process phosphorus metabolic process protein modification process positive regulation of protein phosphorylation positive regulation of MAP kinase activity protein phosphorylation positive regulation of phosphorus metabolic process phosphorylation positive regulation of MAPK cascade regulation of protein metabolic process primary metabolic process positive regulation of intracellular signal transduction regulation of MAPK cascade regulation of molecular function positive regulation of kinase activity regulation of kinase activity regulation of protein serine/threonine kinase activity macromolecule modification regulation of phosphorus metabolic process macromolecule metabolic process regulation of protein modification process regulation of protein phosphorylation positive regulation of protein metabolic process positive regulation of molecular function positive regulation of protein modification process protein metabolic process positive regulation of protein kinase activity positive regulation of transferase activity positive regulation of catalytic activity regulation of protein kinase activity regulation of macromolecule metabolic process MAPK cascade regulation of phosphorylation positive regulation of signaling regulation of cell-substrate adhesion regulation of cell adhesion cell-substrate adhesion negative regulation of cell-matrix adhesion negative regulation of cell adhesion cell adhesion cell-matrix adhesion regulation of cell-matrix adhesion negative regulation of cell-substrate adhesion positive regulation of endothelial cell apoptotic process positive regulation of programmed cell death positive regulation of apoptotic process endothelial cell apoptotic process regulation of endothelial cell apoptotic process import into cell regulation of localization carboxylic acid transmembrane transport transport fatty acid transmembrane transport regulation of lipid transport negative regulation of transport negative regulation of lipid localization negative regulation of transmembrane transport localization long-chain fatty acid import across plasma membrane regulation of long-chain fatty acid import across plasma membrane monocarboxylic acid transport fatty acid transport lipid import into cell long-chain fatty acid import into cell long-chain fatty acid transport organic acid transmembrane transport organic anion transport negative regulation of lipid transport carboxylic acid transport regulation of transport negative regulation of fatty acid transport import across plasma membrane negative regulation of long-chain fatty acid import across plasma membrane regulation of long-chain fatty acid import into cell lipid localization lipid transport establishment of localization regulation of fatty acid transport organic acid transport transmembrane transport regulation of transmembrane transport negative regulation of long-chain fatty acid import into cell regulation of lipid localization negative regulation of organic acid transport macromolecule localization regulation of organic acid transport regulation of tumor necrosis factor production cellular biosynthetic process positive regulation of macromolecule biosynthetic process positive regulation of tumor necrosis factor superfamily cytokine production positive regulation of biosynthetic process cytokine production regulation of cytokine production regulation of biosynthetic process tumor necrosis factor superfamily cytokine production positive regulation of tumor necrosis factor production gene expression macromolecule biosynthetic process tumor necrosis factor production regulation of cellular biosynthetic process regulation of gene expression regulation of tumor necrosis factor superfamily cytokine production biosynthetic process positive regulation of gene expression regulation of macromolecule biosynthetic process positive regulation of cytokine production positive regulation of cellular biosynthetic process negative regulation of cell population proliferation endothelial cell proliferation negative regulation of endothelial cell proliferation regulation of endothelial cell proliferation regulation of epithelial cell proliferation epithelial cell proliferation negative regulation of epithelial cell proliferation peptide cross-linking negative regulation of hydrolase activity negative regulation of proteolysis negative regulation of catalytic activity negative regulation of endopeptidase activity negative regulation of peptidase activity regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of proteolysis proteolysis regulation of endopeptidase activity negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process regulation of cysteine-type endopeptidase activity negative regulation of metabolic process regulation of peptidase activity negative regulation of molecular function regulation of hydrolase activity negative regulation of cysteine-type endopeptidase activity response to metal ion response to calcium ion response to hormone response to steroid hormone response to progesterone regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis blood vessel endothelial cell proliferation involved in sprouting angiogenesis regulation of dendritic cell antigen processing and presentation negative regulation of dendritic cell antigen processing and presentation antigen processing and presentation regulation of antigen processing and presentation negative regulation of immune system process negative regulation of antigen processing and presentation dendritic cell antigen processing and presentation response to decreased oxygen levels response to hypoxia response to oxygen levels nitric oxide-cGMP-mediated signaling negative regulation of chemotaxis endothelial cell chemotaxis regulation of endothelial cell chemotaxis negative regulation of response to external stimulus negative regulation of endothelial cell chemotaxis regulation of cellular component organization cell-substrate junction assembly negative regulation of cell-substrate junction organization cellular component organization cellular component biogenesis cellular component assembly negative regulation of cell junction assembly cell junction assembly regulation of cell-substrate junction organization cellular component organization or biogenesis focal adhesion assembly negative regulation of cellular component organization regulation of cellular component biogenesis negative regulation of focal adhesion assembly regulation of cell junction assembly cell junction organization cell-substrate junction organization regulation of focal adhesion assembly regulation of cell-substrate junction assembly cell surface receptor protein serine/threonine kinase signaling pathway cellular response to transforming growth factor beta stimulus regulation of transmembrane receptor protein serine/threonine kinase signaling pathway regulation of transforming growth factor beta receptor signaling pathway regulation of cellular response to transforming growth factor beta stimulus positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of transmembrane receptor protein serine/threonine kinase signaling pathway positive regulation of cellular response to transforming growth factor beta stimulus response to transforming growth factor beta transforming growth factor beta receptor signaling pathway transforming growth factor beta receptor superfamily signaling pathway regulation of transforming growth factor beta production transforming growth factor beta1 production regulation of transforming growth factor beta1 production positive regulation of transforming growth factor beta1 production transforming growth factor beta production positive regulation of transforming growth factor beta production regulation of fibroblast migration positive regulation of fibroblast migration fibroblast migration ameboidal-type cell migration regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction phosphatidylinositol 3-kinase/protein kinase B signal transduction extrinsic apoptotic signaling pathway via death domain receptors positive regulation of apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of extrinsic apoptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of translation post-transcriptional regulation of gene expression translation regulation of translation negative regulation of wound healing negative regulation of blood coagulation negative regulation of response to wounding negative regulation of hemostasis fibrinolysis regulation of fibrinolysis negative regulation of coagulation negative regulation of fibrinolysis response to mechanical stimulus positive regulation of developmental process positive regulation of angiogenesis positive regulation of vasculature development regulation of protein maturation negative regulation of plasminogen activation negative regulation of protein maturation negative regulation of cellular metabolic process protein maturation regulation of plasminogen activation plasminogen activation zymogen activation negative regulation of cellular biosynthetic process negative regulation of gene expression negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of protein processing protein processing negative regulation of protein processing regulation of interleukin-12 production negative regulation of interleukin-12 production interleukin-12 production negative regulation of cytokine production negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II antigen processing and presentation of peptide or polysaccharide antigen via MHC class II regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II membrane organization vesicle-mediated transport engulfment of apoptotic cell phagocytosis, engulfment membrane invagination phagocytosis apoptotic cell clearance endocytosis plasma membrane invagination response to magnesium ion	membrane-bounded organelle intracellular anatomical structure membrane-enclosed lumen cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle lumen endoplasmic reticulum lumen intracellular organelle organelle lumen organelle endoplasmic reticulum cytoplasm sarcoplasm membrane-bounded organelle intracellular anatomical structure membrane-enclosed lumen cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle lumen endoplasmic reticulum lumen intracellular organelle organelle lumen organelle endoplasmic reticulum cytoplasm sarcoplasm sarcoplasmic reticulum extracellular region extracellular space cell surface cell periphery extracellular matrix external encapsulating structure membrane plasma membrane external side of plasma membrane side of membrane extracellular exosome extracellular vesicle extracellular organelle vesicle extracellular membrane-bounded organelle collagen-containing extracellular matrix intracellular vesicle cytoplasmic vesicle secretory granule secretory vesicle vesicle lumen cytoplasmic vesicle lumen secretory granule lumen platelet alpha granule platelet alpha granule lumen protein-containing complex fibrinogen complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000884	Thrombospondin type-1 (TSP1) repeat	Repeat
A	IPR036383	Thrombospondin type-1 (TSP1) repeat superfamily	Homologous Superfamily
A	IPR003367	Thrombospondin, type 3-like repeat	Repeat
A	IPR001881	EGF-like calcium-binding domain	Domain
A	IPR028974	TSP type-3 repeat	Homologous Superfamily
A	IPR000742	EGF-like domain	Domain
A	IPR017897	Thrombospondin, type 3 repeat	Repeat
A	IPR013320	Concanavalin A-like lectin/glucanase domain superfamily	Homologous Superfamily
A	IPR048287	Thrombospondin-like, N-terminal domain	Domain
A	IPR008859	Thrombospondin, C-terminal	Domain
A	IPR001007	VWFC domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P07996	diabetic retinopathy cirrhosis of liver interstitial nephritis breast cancer diabetic peripheral angiopathy schizophrenia juvenile idiopathic arthritis breast carcinoma Fibrosis psoriasis glioblastoma tuberculosis interstitial cystitis ischemia reperfusion injury adrenal cortex adenoma diabetic retinopathy cirrhosis of liver interstitial nephritis breast cancer diabetic peripheral angiopathy schizophrenia juvenile idiopathic arthritis breast carcinoma Fibrosis psoriasis glioblastoma tuberculosis interstitial cystitis ischemia reperfusion injury adrenal cortex adenoma lung cancer atypical teratoid rhabdoid tumor malignant mesothelioma cancer MALT lymphoma medulloblastoma osteosarcoma cystic fibrosis pancreatic intraductal papillary-mucinous carcinoma Emery-Dreifuss muscular dystrophy pituitary cancer head and neck cancer ulcerative colitis adrenal cortex carcinoma lung carcinoma chronic obstructive pulmonary disease congestive heart failure ovarian cancer pancreatic intraductal papillary-mucinous neoplasm esophageal adenocarcinoma polycystic ovary syndrome gastric carcinoma hepatocellular carcinoma invasive ductal breast carcinoma large cell medulloblastoma adrenoleukodystrophy malignant colon neoplasm malignant pancreatic neoplasm Crohn disease nasopharyngeal carcinoma pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.