2GTP

Crystal structure of the heterodimeric complex of human RGS1 and activated Gi alpha 1

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2gtpa1	All alpha proteins	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2gtpa2	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Transducin (alpha subunit)	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2gtpb1	All alpha proteins	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2gtpb2	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Transducin (alpha subunit)	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d2gtpc_	All alpha proteins	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d2gtpd_	All alpha proteins	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056779	3002022	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056779	3002022	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Galpha insert domain-like	8056780	3000669	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ras-like P-loop GTPases	8056779	3002022	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ras-like P-loop GTPases	8056779	3002022	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Galpha insert domain-like	8056780	3000669	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Regulator of G-protein signaling, RGS	8062635	3000835	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Regulator of G-protein signaling, RGS	8062635	3000835	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	G-alpha	e2gtpA1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
A	Ras	e2gtpA2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
B	G-alpha	e2gtpB1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
B	Ras	e2gtpB2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
C	RGS	e2gtpC1	A: alpha bundles	X: Regulator of G-protein signaling, RGS (From Topology)	H: Regulator of G-protein signaling, RGS (From Topology)	T: Regulator of G-protein signaling, RGS	F: RGS	ECOD (1.6)
D	RGS	e2gtpD1	A: alpha bundles	X: Regulator of G-protein signaling, RGS (From Topology)	H: Regulator of G-protein signaling, RGS (From Topology)	T: Regulator of G-protein signaling, RGS	F: RGS	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
C	1.10.196.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 1	CATH (4.3.0)
C	1.10.167.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 2	CATH (4.3.0)
D	1.10.196.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 1	CATH (4.3.0)
D	1.10.167.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00503	G-protein alpha subunit (G-alpha)	G-protein alpha subunit	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like ...	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like family form a ring of residues centered on the nucleotide binding site [3]. A Ggamma is found fused to an inactive Galpha in the Dictyostelium protein gbqA [3].	Domain
C, D	PF00615	Regulator of G protein signaling domain (RGS)	Regulator of G protein signaling domain	RGS family members are GTPase-activating proteins for heterotrimeric G-protein alpha-subunits.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Guanine nucleotide-binding protein G(i), alpha-1 subunit	D2 dopamine receptor binding protein binding binding signaling receptor binding G protein-coupled receptor binding dopamine receptor binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding D2 dopamine receptor binding protein binding binding signaling receptor binding G protein-coupled receptor binding dopamine receptor binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding cation binding magnesium ion binding metal ion binding GDP binding G protein-coupled serotonin receptor binding protein-containing complex binding G-protein beta/gamma-subunit complex binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	response to stimulus response to hormone response to chemical response to endogenous stimulus response to peptide hormone response to oxygen-containing compound response to nitrogen compound cellular process cell division leukocyte migration immune system process mononuclear cell migration lymphocyte migration cell migration response to stimulus response to hormone response to chemical response to endogenous stimulus response to peptide hormone response to oxygen-containing compound response to nitrogen compound cellular process cell division leukocyte migration immune system process mononuclear cell migration lymphocyte migration cell migration T cell migration cell motility cellular response to stimulus regulation of biological process regulation of cellular process signaling biological regulation cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction regulation of cytoskeleton organization regulation of cellular component organization microtubule cytoskeleton organization regulation of mitotic spindle organization mitotic cell cycle cellular component organization cell cycle cellular component organization or biogenesis regulation of cell cycle microtubule cytoskeleton organization involved in mitosis spindle organization cell cycle process regulation of cell cycle process regulation of microtubule-based process regulation of spindle organization regulation of organelle organization mitotic spindle organization organelle organization mitotic cell cycle process microtubule-based process cytoskeleton organization regulation of microtubule cytoskeleton organization protein localization positive regulation of protein localization to cell periphery regulation of localization protein localization to cell periphery cellular localization regulation of protein localization positive regulation of biological process positive regulation of protein localization to cell cortex regulation of protein localization to cell periphery localization regulation of cellular localization regulation of protein localization to cell cortex cellular macromolecule localization macromolecule localization positive regulation of protein localization protein localization to cell cortex positive regulation of cellular process adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway regulation of signaling regulation of signal transduction intracellular signal transduction cAMP-mediated signaling regulation of intracellular signal transduction regulation of response to stimulus intracellular signaling cassette regulation of cell communication regulation of cAMP-mediated signaling response to ketone response to lipid response to alcohol cellular response to forskolin cellular response to organic cyclic compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to alcohol response to forskolin cellular response to lipid cellular response to ketone response to organic cyclic compound	midbody cellular anatomical entity membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen midbody cellular anatomical entity membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen non-membrane-bounded organelle cell periphery cell cortex cytoplasm protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle centrosome microtubule organizing center cytoskeleton microtubule cytoskeleton nucleoplasm vacuolar membrane lytic vacuole vacuole lytic vacuole membrane bounding membrane of organelle lysosomal membrane organelle membrane lysosome
C, D	Regulator of G-protein signaling 1	protein binding binding G-protein alpha-subunit binding calmodulin binding GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity protein binding binding G-protein alpha-subunit binding calmodulin binding GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	cellular response to stimulus regulation of signaling regulation of biological process regulation of cellular process response to stimulus negative regulation of signaling signaling negative regulation of response to stimulus biological regulation negative regulation of biological process regulation of signal transduction negative regulation of signal transduction negative regulation of cell communication cell communication regulation of response to stimulus cellular response to stimulus regulation of signaling regulation of biological process regulation of cellular process response to stimulus negative regulation of signaling signaling negative regulation of response to stimulus biological regulation negative regulation of biological process regulation of signal transduction negative regulation of signal transduction negative regulation of cell communication cell communication regulation of response to stimulus negative regulation of cellular process regulation of cell communication cellular process signal transduction immune response immune system process biological process involved in interspecies interaction between organisms response to other organism response to bacterium response to external stimulus response to external biotic stimulus response to biotic stimulus adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway positive regulation of molecular function regulation of molecular function positive regulation of hydrolase activity regulation of catalytic activity regulation of hydrolase activity positive regulation of catalytic activity regulation of GTPase activity positive regulation of GTPase activity leukotriene signaling pathway	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol cell periphery plasma membrane membrane cytoplasmic side of plasma membrane cytoplasmic side of membrane side of membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
A, B	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
A, B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B	IPR001408	G-protein alpha subunit, group I	Family
C, D	IPR016137	RGS domain	Domain
C, D	IPR036305	RGS domain superfamily	Homologous Superfamily
C, D	IPR044926	RGS, subdomain 2	Homologous Superfamily
C, D	IPR024066	RGS, subdomain 1/3	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P63096	substance withdrawal syndrome dissociative amnesia malignant glioma nicotine dependence pancreatic ductal adenocarcinoma acute myeloid leukemia Withdrawal Symptoms breast cancer glioblastoma intellectual disability large cell medulloblastoma malignant pancreatic neoplasm oligodendroglioma Pick disease astrocytic tumor substance withdrawal syndrome dissociative amnesia malignant glioma nicotine dependence pancreatic ductal adenocarcinoma acute myeloid leukemia Withdrawal Symptoms breast cancer glioblastoma intellectual disability large cell medulloblastoma malignant pancreatic neoplasm oligodendroglioma Pick disease astrocytic tumor breast carcinoma interstitial cystitis malignant colon neoplasm medulloblastoma ovarian cancer subependymal giant cell astrocytoma amnestic disorder atypical teratoid rhabdoid tumor breast fibroadenoma ductal breast carcinoma in situ invasive ductal breast carcinoma
C, D	Pharos: Q08116	osteosarcoma allergic contact dermatitis Arsenic Poisoning large cell medulloblastoma malignant ependymoma marginal zone lymphoma non-small cell lung carcinoma ovarian cancer pilocytic astrocytoma atopic eczema Sjogren syndrome celiac disease tuberculosis ductal breast carcinoma in situ hepatocellular carcinoma osteosarcoma allergic contact dermatitis Arsenic Poisoning large cell medulloblastoma malignant ependymoma marginal zone lymphoma non-small cell lung carcinoma ovarian cancer pilocytic astrocytoma atopic eczema Sjogren syndrome celiac disease tuberculosis ductal breast carcinoma in situ hepatocellular carcinoma lung cancer malignant glioma medulloblastoma oligodendroglioma pancreatic intraductal papillary-mucinous carcinoma plasma cell myeloma breast cancer skin disease cutaneous lupus erythematosus ulcerative colitis diffuse large B-cell lymphoma esophageal adenocarcinoma invasive ductal breast carcinoma lung carcinoma malignant pancreatic neoplasm periodontitis astrocytic tumor psoriasis breast carcinoma transitional cell carcinoma depressive disorder Down syndrome glioblastoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.