2NL9

Crystal structure of the Mcl-1:Bim BH3 complex

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2nl9a_	Membrane and cell surface proteins and peptides	Toxins' membrane translocation domains	Bcl-2 inhibitors of programmed cell death	Bcl-2 inhibitors of programmed cell death	EAT/MCL-1 (Myeloid cell leukemia sequence 1)	Mouse (Mus musculus ) [TaxId: 10090 ], human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	BH3 domain	8055788	3002075	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Bcl-2	e2nl9A1	A: alpha arrays	X: Toxins' membrane translocation domains	H: Bcl-2 inhibitors of programmed cell death (From Topology)	T: Bcl-2 inhibitors of programmed cell death	F: Bcl-2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.437.10	Mainly Alpha	Orthogonal Bundle	Apoptosis Regulator Bcl-x	Blc2-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00452	Apoptosis regulator proteins, Bcl-2 family (Bcl-2)	Apoptosis regulator proteins, Bcl-2 family	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B	Bcl-2-like protein 11	cytoskeletal protein binding microtubule binding protein binding binding tubulin binding kinase binding enzyme binding protein kinase binding	developmental process multicellular organismal process gonad development reproductive system development reproductive process sex differentiation animal organ development developmental process involved in reproduction male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development developmental process multicellular organismal process gonad development reproductive system development reproductive process sex differentiation animal organ development developmental process involved in reproduction male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development system development spermatogenesis sexual reproduction multicellular organismal reproductive process male gamete generation gamete generation cellular response to stimulus positive regulation of organelle organization regulation of cellular process regulation of cellular component organization positive regulation of cellular component organization response to stimulus signaling apoptotic mitochondrial changes positive regulation of biological process regulation of release of cytochrome c from mitochondria cell communication cellular component organization positive regulation of release of cytochrome c from mitochondria cellular component organization or biogenesis signal transduction positive regulation of cellular process mitochondrion organization regulation of biological process biological regulation cell death regulation of organelle organization apoptotic signaling pathway release of cytochrome c from mitochondria organelle organization regulation of mitochondrion organization cellular process apoptotic process programmed cell death protein-containing complex organization positive regulation of cellular component biogenesis regulation of cellular component biogenesis cellular component biogenesis positive regulation of protein-containing complex assembly regulation of protein-containing complex assembly cellular component assembly protein-containing complex assembly positive regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death positive regulation of apoptotic process neuron apoptotic process positive regulation of neuron apoptotic process regulation of neuron apoptotic process B cell homeostasis multicellular organismal-level homeostasis leukocyte homeostasis homeostatic process immune system process homeostasis of number of cells lymphocyte homeostasis mammary gland development gland development embryo development ending in birth or egg hatching chordate embryonic development embryo development in utero embryonic development pigmentation developmental pigmentation signal transduction in absence of ligand cell surface receptor signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand fibroblast apoptotic process positive regulation of fibroblast apoptotic process regulation of fibroblast apoptotic process positive regulation of IRE1-mediated unfolded protein response positive regulation of cell communication positive regulation of signal transduction regulation of cellular response to stress endoplasmic reticulum unfolded protein response regulation of response to endoplasmic reticulum stress regulation of signal transduction intracellular signal transduction positive regulation of response to stimulus regulation of endoplasmic reticulum unfolded protein response positive regulation of response to endoplasmic reticulum stress cellular response to stress regulation of intracellular signal transduction response to stress regulation of response to stress positive regulation of endoplasmic reticulum unfolded protein response response to endoplasmic reticulum stress regulation of IRE1-mediated unfolded protein response regulation of signaling cellular response to topologically incorrect protein positive regulation of intracellular signal transduction cellular response to unfolded protein response to unfolded protein regulation of response to stimulus response to topologically incorrect protein IRE1-mediated unfolded protein response regulation of cell communication positive regulation of signaling immune system development spleen development hematopoietic or lymphoid organ development regulation of intrinsic apoptotic signaling pathway positive regulation of apoptotic signaling pathway intrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway regulation of apoptotic signaling pathway regulation of leukocyte apoptotic process regulation of lymphocyte apoptotic process positive regulation of lymphocyte apoptotic process leukocyte apoptotic process positive regulation of T cell apoptotic process lymphocyte apoptotic process regulation of T cell apoptotic process T cell apoptotic process positive regulation of leukocyte apoptotic process thymocyte apoptotic process kidney development renal system development cell cycle regulation of cell cycle positive regulation of cell cycle apoptotic process involved in morphogenesis limb development appendage development embryonic limb morphogenesis embryonic morphogenesis embryonic appendage morphogenesis apoptotic process involved in development anatomical structure morphogenesis apoptotic process involved in embryonic digit morphogenesis appendage morphogenesis embryonic digit morphogenesis limb morphogenesis cell adhesion cell-matrix adhesion cell-substrate adhesion thymus development ear development sensory organ development odontogenesis of dentin-containing tooth odontogenesis animal organ morphogenesis meiotic cell cycle organelle fission meiotic cell cycle process nuclear division meiosis I meiosis I cell cycle process meiotic nuclear division cell cycle process regulation of developmental pigmentation regulation of pigmentation T cell homeostasis intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway regulation of growth regulation of developmental growth regulation of developmental process developmental growth growth organ growth regulation of organ growth regulation of multicellular organismal process myeloid cell homeostasis DNA damage response intrinsic apoptotic signaling pathway in response to DNA damage transport mitochondrial outer membrane permeabilization involved in programmed cell death mitochondrial membrane organization localization positive regulation of mitochondrial membrane permeability involved in apoptotic process positive regulation of mitochondrial membrane permeability positive regulation of membrane permeability regulation of biological quality regulation of mitochondrial membrane permeability mitochondrial transport membrane organization establishment of localization regulation of membrane permeability regulation of mitochondrial membrane permeability involved in apoptotic process post-embryonic development response to lipid response to endogenous stimulus response to glucocorticoid cellular response to organic cyclic compound cellular response to glucocorticoid stimulus cellular response to corticosteroid stimulus cellular response to endogenous stimulus response to corticosteroid cellular response to chemical stimulus response to hormone response to chemical response to steroid hormone cellular response to lipid cellular response to steroid hormone stimulus response to organic cyclic compound cellular response to hormone stimulus tube morphogenesis tube development anatomical structure formation involved in morphogenesis tube formation	protein-containing complex BIM-BCL-2 complex Bcl-2 family protein complex cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle mitochondrial membrane mitochondrion membrane organelle outer membrane intracellular membrane-bounded organelle intracellular organelle protein-containing complex BIM-BCL-2 complex Bcl-2 family protein complex cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle mitochondrial membrane mitochondrion membrane organelle outer membrane intracellular membrane-bounded organelle intracellular organelle organelle envelope bounding membrane of organelle organelle mitochondrial envelope mitochondrial outer membrane outer membrane organelle membrane endomembrane system BIM-BCL-xl complex
A	FUSION PROTEIN CONSISTING OF Induced myeloid leukemia cell differentiation protein Mcl-1 homolog	protein heterodimerization activity protein binding binding protein dimerization activity BH domain binding protein domain specific binding death domain binding BH3 domain binding protein-containing complex binding passive transmembrane transporter activity channel activity transporter activity transmembrane transporter activity macromolecule transmembrane transporter activity protein heterodimerization activity protein binding binding protein dimerization activity BH domain binding protein domain specific binding death domain binding BH3 domain binding protein-containing complex binding passive transmembrane transporter activity channel activity transporter activity transmembrane transporter activity macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process positive regulation of cell communication intrinsic apoptotic signaling pathway in response to oxidative stress neuron apoptotic process regulation of cellular process positive regulation of signal transduction regulation of oxidative stress-induced intrinsic apoptotic signaling pathway signaling positive regulation of biological process cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process positive regulation of cell communication intrinsic apoptotic signaling pathway in response to oxidative stress neuron apoptotic process regulation of cellular process positive regulation of signal transduction regulation of oxidative stress-induced intrinsic apoptotic signaling pathway signaling positive regulation of biological process regulation of signal transduction regulation of programmed cell death regulation of intrinsic apoptotic signaling pathway positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway intracellular signal transduction cell communication positive regulation of apoptotic signaling pathway positive regulation of response to stimulus regulation of intracellular signal transduction signal transduction intrinsic apoptotic signaling pathway positive regulation of neuron apoptotic process positive regulation of cellular process regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway regulation of biological process regulation of signaling positive regulation of programmed cell death regulation of apoptotic process biological regulation positive regulation of intracellular signal transduction cell death positive regulation of apoptotic process neuron intrinsic apoptotic signaling pathway in response to oxidative stress apoptotic signaling pathway regulation of response to stimulus positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway regulation of cell communication positive regulation of intrinsic apoptotic signaling pathway apoptotic process programmed cell death regulation of neuron apoptotic process regulation of apoptotic signaling pathway positive regulation of signaling developmental process cellular developmental process cell differentiation negative regulation of extrinsic apoptotic signaling pathway in absence of ligand signal transduction in absence of ligand negative regulation of signaling negative regulation of biological process negative regulation of cell communication extrinsic apoptotic signaling pathway negative regulation of signal transduction in absence of ligand negative regulation of extrinsic apoptotic signaling pathway negative regulation of apoptotic signaling pathway negative regulation of cellular process extrinsic apoptotic signaling pathway in absence of ligand cell surface receptor signaling pathway negative regulation of response to stimulus negative regulation of programmed cell death negative regulation of signal transduction regulation of extrinsic apoptotic signaling pathway negative regulation of apoptotic process regulation of extrinsic apoptotic signaling pathway in absence of ligand organelle fusion cellular component organization organelle organization mitochondrial fusion cellular component organization or biogenesis mitochondrion organization regulation of anoikis negative regulation of anoikis anoikis response to peptide response to chemical response to cytokine intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of catabolic process catabolic process negative regulation of metabolic process autophagy regulation of catabolic process regulation of autophagy negative regulation of autophagy metabolic process process utilizing autophagic mechanism regulation of metabolic process apoptotic mitochondrial changes release of cytochrome c from mitochondria cellular homeostasis homeostatic process cell fate determination cell fate commitment	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle membrane cytosol protein-containing complex Bcl-2 family protein complex nucleus membrane-enclosed lumen membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle membrane cytosol protein-containing complex Bcl-2 family protein complex nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen mitochondrial membrane organelle outer membrane organelle envelope bounding membrane of organelle mitochondrial envelope mitochondrial outer membrane outer membrane organelle membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B	IPR014771	Apoptosis, Bim N-terminal	Domain
B	IPR017288	Bcl-2-like protein 11	Family
B	IPR015040	Bcl-x interacting, BH3 domain	Domain
B	IPR052133	Immune Signaling and Apoptosis Regulator	Family
A	IPR020726	Apoptosis regulator, Bcl-2, BH2 motif, conserved site	Conserved Site
A	IPR036834	Bcl-2-like superfamily	Homologous Superfamily
A	IPR046371	Bcl-2, Bcl-2 homology region 1-3	Domain
A	IPR002475	Bcl2-like	Family
A	IPR026298	Bcl-2 family	Family
A	IPR020717	Apoptosis regulator, Bcl-2, BH1 motif, conserved site	Conserved Site
A	IPR013281	Apoptosis regulator, Mcl-1	Family
A	IPR020728	Apoptosis regulator, Bcl-2, BH3 motif, conserved site	Conserved Site
A	IPR020726	Apoptosis regulator, Bcl-2, BH2 motif, conserved site	Conserved Site
A	IPR036834	Bcl-2-like superfamily	Homologous Superfamily
A	IPR046371	Bcl-2, Bcl-2 homology region 1-3	Domain
A	IPR002475	Bcl2-like	Family
A	IPR026298	Bcl-2 family	Family
A	IPR020717	Apoptosis regulator, Bcl-2, BH1 motif, conserved site	Conserved Site
A	IPR013281	Apoptosis regulator, Mcl-1	Family
A	IPR020728	Apoptosis regulator, Bcl-2, BH3 motif, conserved site	Conserved Site

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B	Pharos: O43521	sclerosing cholangitis primary sclerosing cholangitis malignant mesothelioma medulloblastoma osteosarcoma pancreatic ductal adenocarcinoma adenoma primitive neuroectodermal tumor B-cell chronic lymphocytic leukemia Diabetes Mellitus, Non-Insulin-Dependent ulcerative colitis gastric cancer hematologic disease large cell medulloblastoma malignant ependymoma sclerosing cholangitis primary sclerosing cholangitis malignant mesothelioma medulloblastoma osteosarcoma pancreatic ductal adenocarcinoma adenoma primitive neuroectodermal tumor B-cell chronic lymphocytic leukemia Diabetes Mellitus, Non-Insulin-Dependent ulcerative colitis gastric cancer hematologic disease large cell medulloblastoma malignant ependymoma malignant pancreatic neoplasm non-small cell lung carcinoma ovarian cancer pilocytic astrocytoma astrocytic tumor breast carcinoma tuberculosis eosinophilic granulomatosis with polyangiitis vitiligo gastric carcinoma hepatocellular carcinoma lung cancer malignant glioma MALT lymphoma oligodendroglioma ovarian carcinoma atypical teratoid rhabdoid tumor prostate carcinoma cancer type 2 diabetes mellitus exocrine pancreatic carcinoma glioblastoma invasive ductal breast carcinoma lymphatic system disease
A	Pharos: Q07820	Barrett esophagus plasma cell myeloma leishmaniasis spindle cell sarcoma primary cutaneous T-cell non-Hodgkin lymphoma sarcoma subependymal giant cell astrocytoma interstitial lung disease lung carcinoma Lymphoma, T-Cell, Cutaneous adenoma myeloid neoplasm cancer osteosarcoma Pick disease Barrett esophagus plasma cell myeloma leishmaniasis spindle cell sarcoma primary cutaneous T-cell non-Hodgkin lymphoma sarcoma subependymal giant cell astrocytoma interstitial lung disease lung carcinoma Lymphoma, T-Cell, Cutaneous adenoma myeloid neoplasm cancer osteosarcoma Pick disease diabetes mellitus plasma cell neoplasm gastric cancer primitive neuroectodermal tumor glioblastoma sinusitis hereditary spastic paraplegia ulcerative colitis invasive ductal breast carcinoma lung benign neoplasm malignant ependymoma astrocytic tumor malignant pancreatic neoplasm non-small cell lung carcinoma ovarian cancer chronic obstructive pulmonary disease pilocytic astrocytoma Down syndrome polycystic ovary syndrome gastric carcinoma psoriasis granulomatous slack skin disease interstitial cystitis large cell medulloblastoma lung cancer lymphatic system disease malignant glioma atypical teratoid rhabdoid tumor medulloblastoma breast carcinoma osteoarthritis pancreatic ductal adenocarcinoma cutaneous lupus erythematosus epithelioid sarcoma Gaucher disease hematologic disease