2QMJ

Crystral Structure of the N-terminal Subunit of Human Maltase-Glucoamylase in Complex with Acarbose

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2qmja1	All beta proteins	Supersandwich	Galactose mutarotase-like	Glycosyl hydrolase family 31, N-terminal domain-like	N-terminal subunit of Maltase-glucoamylase (NtMGAM), N-terminal domain	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2qmja3	All beta proteins	Glycosyl hydrolase domain	Glycosyl hydrolase domain	Glycosyl hydrolase family 31, domain 3	N-terminal subunit of Maltase-glucoamylase (NtMGAM), domain 3	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2qmja4	All beta proteins	Glycolsyl hydrolase family 31 C-terminal domain-like	Glycolsyl hydrolase family 31 C-terminal domain	Glycolsyl hydrolase family 31 C-terminal domain	N-terminal subunit of Maltase-glucoamylase (NtMGAM), C-terminal domain	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2qmja2	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	(Trans)glycosidases	Glycosyl hydrolase family 31 catalytic domain	N-terminal subunit of Maltase-glucoamylase (NtMGAM), catalytic domain	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2qmja5	Artifacts	Tags	Tags	Tags	C-terminal Tags	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Glycosyl hydrolase domain-like	8093223	3001528	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Galactose mutarotase-like	8093217	3000035	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Trefoil	8093219	3000849	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	(Trans)glycosidases	8093221	3000313	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	YicI C-terminal domain-like	8093225	3000267	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Glyco_hydro_31_C	e2qmjA2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Glyco_hydro_31_C	ECOD (1.6)
A	Gal_mutarotas_2	e2qmjA5	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Gal_mutarotas_2	ECOD (1.6)
A	DUF5110_1	e2qmjA4	A: beta sandwiches	X: Putative glucosidase YicI, C-terminal domain (From Topology)	H: Putative glucosidase YicI, C-terminal domain (From Topology)	T: Putative glucosidase YicI, C-terminal domain	F: DUF5110_1	ECOD (1.6)
A	Glyco_hydro_31_N	e2qmjA1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glyco_hydro_31_N	ECOD (1.6)
A	Trefoil	e2qmjA6	A: few secondary structure elements	X: Trefoil/Plexin domain-like (From Topology)	H: Trefoil/Plexin domain-like (From Topology)	T: Trefoil/Plexin domain-like	F: Trefoil	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	4.10.110.10	Few Secondary Structures	Irregular	Spasmolytic Protein	domain 1	CATH (4.3.0)
A	2.60.40.1760	Mainly Beta	Sandwich	Immunoglobulin-like	glycosyl hydrolase (family 31)	CATH (4.3.0)
A	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
A	2.60.40.1180	Mainly Beta	Sandwich	Immunoglobulin-like	Golgi alpha-mannosidase II	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF21365	Glycosyl hydrolase family 31 C-terminal domain (Glyco_hydro_31_3rd)	Glycosyl hydrolase family 31 C-terminal domain	Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. This entry represents the 3rd beta sandwich domain.	Domain
A	PF13802	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain (Gal_mutarotas_2)	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain	This entry represents the first of three common domains found in Glycosyl hydrolase 31 family proteins. It has a galactose mutarotase superfamily fold.	Domain
A	PF01055	Glycosyl hydrolases family 31 TIM-barrel domain (Glyco_hydro_31_2nd)	Glycosyl hydrolases family 31 TIM-barrel domain	Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. This entry corresponds to the second domain of family 31 enzymes and adopts a TIM-barrel fold.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Maltase-glucoamylase, intestinal	hydrolase activity, acting on glycosyl bonds amylase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity catalytic activity alpha-glucosidase activity glucosidase activity alpha-1,4-glucosidase activity binding carbohydrate binding glucan 1,4-alpha-glucosidase activity	carbohydrate metabolic process catabolic process glucan catabolic process carbohydrate catabolic process primary metabolic process macromolecule catabolic process polysaccharide metabolic process glucan metabolic process starch metabolic process polysaccharide catabolic process starch catabolic process metabolic process cellular process macromolecule metabolic process carbohydrate metabolic process catabolic process glucan catabolic process carbohydrate catabolic process primary metabolic process macromolecule catabolic process polysaccharide metabolic process glucan metabolic process starch metabolic process polysaccharide catabolic process starch catabolic process metabolic process cellular process macromolecule metabolic process maltose catabolic process oligosaccharide catabolic process oligosaccharide metabolic process disaccharide metabolic process maltose metabolic process disaccharide catabolic process dextrin catabolic process	membrane-bounded organelle intracellular anatomical structure tertiary granule membrane membrane intracellular vesicle intracellular membrane-bounded organelle endomembrane system tertiary granule intracellular organelle cytoplasmic vesicle membrane cellular anatomical structure bounding membrane of organelle vesicle membrane cytoplasmic vesicle membrane-bounded organelle intracellular anatomical structure tertiary granule membrane membrane intracellular vesicle intracellular membrane-bounded organelle endomembrane system tertiary granule intracellular organelle cytoplasmic vesicle membrane cellular anatomical structure bounding membrane of organelle vesicle membrane cytoplasmic vesicle organelle secretory granule secretory vesicle cytoplasm vesicle organelle membrane secretory granule membrane cell periphery apical part of cell plasma membrane plasma membrane region apical plasma membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space extracellular membrane-bounded organelle ficolin-1-rich granule membrane ficolin-1-rich granule

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR048395	Glycosyl hydrolase family 31, C-terminal domain	Domain
A	IPR013780	Glycosyl hydrolase, all-beta	Homologous Superfamily
A	IPR025887	Glycoside hydrolase family 31, N-terminal domain	Domain
A	IPR044913	P-type trefoil domain superfamily	Homologous Superfamily
A	IPR030459	Glycosyl hydrolases family 31, conserved site	Conserved Site
A	IPR000322	Glycoside hydrolase family 31, TIM barrel domain	Domain
A	IPR017853	Glycoside hydrolase superfamily	Homologous Superfamily
A	IPR000519	P-type trefoil domain	Domain
A	IPR030458	Glycosyl hydrolases family 31, active site	Active Site
A	IPR017957	P-type trefoil, conserved site	Conserved Site
A	IPR011013	Galactose mutarotase-like domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: O43451	type 2 diabetes mellitus cystic fibrosis hyperglycemia lung cancer MALT lymphoma osteosarcoma DOID:0081062 hypoglycemia lung carcinoma nephrosclerosis rheumatoid arthritis glycogen storage disease lung adenocarcinoma malignant mesothelioma non-small cell lung carcinoma type 2 diabetes mellitus cystic fibrosis hyperglycemia lung cancer MALT lymphoma osteosarcoma DOID:0081062 hypoglycemia lung carcinoma nephrosclerosis rheumatoid arthritis glycogen storage disease lung adenocarcinoma malignant mesothelioma non-small cell lung carcinoma tuberculosis

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	α‑glucosidase maltase-glucoamylase M-CSA #976	α‑Glucosidase Maltase- Glucoamylase catalyses the digestion of starch. α‑Glucosidase inhibitors have been efficient in delaying glucose production, which is crucial for regulating postprandial blood glucose levels and can be used for therapeutic purposes to reduce the damage and complications in diabetics and obesity.	Defined by 10 residues: TYR:A-214ASP:A-327ASP:A-366TRP:A-406ASP:A-443MET:A-444ARG:A-526TRP:A-539ASP:A-542HIS:A-600 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.2.1 (PDB Primary Data) Search M-CSA Motif + EC 3.2.1 EC: 3.2.1.20 (UniProt) Search M-CSA Motif + EC 3.2.1.20

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.