Annotations: 3AI5

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	GFP-like	8096046	3000576	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	GFP	e3ai5A1	A: beta barrels	X: GFP-like (From Topology)	H: GFP-like (From Topology)	T: GFP-like	F: GFP	ECOD (1.6)
A	ubiquitin_1	e3ai5A2	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.40.155.10	Mainly Beta	Beta Barrel	Green Fluorescent Protein	Green fluorescent protein	CATH (4.3.0)
A	3.10.20.90	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Phosphatidylinositol 3-kinase Catalytic Subunit	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00240	Ubiquitin family (ubiquitin)	Ubiquitin family	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry ...	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry a distinctive five-residue motif termed the proteasome-interacting motif (PIM), which may have a biologically significant role in protein delivery to proteasomes and recruitment of proteasomes to transcription sites [5].	Domain
A	PF01353	Green fluorescent protein (GFP)	Green fluorescent protein	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	yeast enhanced green fluorescent protein,Ubiquitin	structural molecule activity structural constituent of ribosome protein tag activity molecular tag activity ubiquitin protein ligase binding protein binding binding ubiquitin-like protein ligase binding enzyme binding	bioluminescence cellular metabolic process metabolic process cellular process generation of precursor metabolites and energy translation at synapse cellular biosynthetic process primary metabolic process translation at presynapse translation gene expression macromolecule biosynthetic process biosynthetic process protein metabolic process bioluminescence cellular metabolic process metabolic process cellular process generation of precursor metabolites and energy translation at synapse cellular biosynthetic process primary metabolic process translation at presynapse translation gene expression macromolecule biosynthetic process biosynthetic process protein metabolic process macromolecule metabolic process catabolic process proteolysis involved in protein catabolic process protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis protein ubiquitination macromolecule modification post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal cytoplasmic translation translation at postsynapse	myelin sheath cellular anatomical entity intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane membrane presynapse synapse cell junction membrane-bounded organelle nucleus membrane-enclosed lumen myelin sheath cellular anatomical entity intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane membrane presynapse synapse cell junction membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen intracellular non-membrane-bounded organelle ribosome non-membrane-bounded organelle protein-containing complex ribosomal subunit cytosolic large ribosomal subunit cytosolic ribosome ribonucleoprotein complex large ribosomal subunit cytosolic small ribosomal subunit small ribosomal subunit postsynapse endoplasmic reticulum endomembrane system

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000786	Green fluorescent protein, GFP	Family
A	IPR011584	Green fluorescent protein-related	Family
A	IPR009017	Green fluorescent protein	Homologous Superfamily
A	IPR019954	Ubiquitin conserved site	Conserved Site
A	IPR038587	Large ribosomal subunit protein eL40 superfamily	Homologous Superfamily
A	IPR050158	Ubiquitin and ubiquitin-like	Family
A	IPR000626	Ubiquitin-like domain	Domain
A	IPR019956	Ubiquitin domain	Domain
A	IPR001975	Large ribosomal subunit protein eL40 domain	Domain
A	IPR029071	Ubiquitin-like domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.