3B1X

Crystal structure of an S. thermophilus NFeoB E66A mutant bound to GMPPNP

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8058456	3002022	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ras-like P-loop GTPases	8058456	3002022	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	FeoB	e3b1xA4	A: alpha complex topology	X: Cytosolic helical domain in ferrous iron transport protein B (From Topology)	H: Cytosolic helical domain in ferrous iron transport protein B (From Topology)	T: Cytosolic helical domain in ferrous iron transport protein B	F: FeoB	ECOD (1.6)
A	MnmE_helical_2nd	e3b1xA3	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: MnmE_helical_2nd	ECOD (1.6)
B	FeoB	e3b1xB4	A: alpha complex topology	X: Cytosolic helical domain in ferrous iron transport protein B (From Topology)	H: Cytosolic helical domain in ferrous iron transport protein B (From Topology)	T: Cytosolic helical domain in ferrous iron transport protein B	F: FeoB	ECOD (1.6)
B	MnmE_helical_2nd	e3b1xB3	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: MnmE_helical_2nd	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.287.1770	Mainly Alpha	Orthogonal Bundle	Helix Hairpins		CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	1.10.287.1770	Mainly Alpha	Orthogonal Bundle	Helix Hairpins		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF02421	Ferrous iron transport protein B (FeoB_N)	Ferrous iron transport protein B	Escherichia coli has an iron(II) transport system (feo) which may make an important contribution to the iron supply of the cell under anaerobic conditions [1]. FeoB has been identified as part of this transport system. FeoB is a large 700-800 amino a ...	Escherichia coli has an iron(II) transport system (feo) which may make an important contribution to the iron supply of the cell under anaerobic conditions [1]. FeoB has been identified as part of this transport system. FeoB is a large 700-800 amino acid integral membrane protein. The N terminus contains a P-loop motif suggesting that iron transport may be ATP dependent [1].	Domain
A, B	PF17910	FeoB cytosolic helical domain (FeoB_Cyto)	FeoB cytosolic helical domain	FeoB is a G-protein coupled membrane protein essential for Fe(II) uptake in prokaryotes. In the structures, a canonical G-protein domain (G domain) is followed by a helical bundle domain (S-domain) which is represented by this entry.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Ferrous iron uptake transporter protein B	monoatomic cation transmembrane transporter activity inorganic molecular entity transmembrane transporter activity iron ion transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity ferrous iron transmembrane transporter activity transporter activity transition metal ion transmembrane transporter activity metal ion transmembrane transporter activity transmembrane transporter activity binding guanyl nucleotide binding GTP binding nucleotide binding monoatomic cation transmembrane transporter activity inorganic molecular entity transmembrane transporter activity iron ion transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity ferrous iron transmembrane transporter activity transporter activity transition metal ion transmembrane transporter activity metal ion transmembrane transporter activity transmembrane transporter activity binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding cation binding metal ion binding	-	cell periphery plasma membrane membrane cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR050860	Fe(2+) Transporter FeoB GTPase	Family
A, B	IPR030389	FeoB-type guanine nucleotide-binding (G) domain	Domain
A, B	IPR003373	Ferrous iron transport protein B	Family
A, B	IPR011640	Ferrous iron transport protein B, C-terminal	Domain
A, B	IPR006073	GTP binding domain	Domain
A, B	IPR011642	Nucleoside transporter/FeoB GTPase, Gate domain	Domain
A, B	IPR041069	FeoB, cytosolic helical domain	Domain
A, B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.