3CBL

Crystal structure of human feline sarcoma viral oncogene homologue (v-FES) in complex with staurosporine and a consensus peptide

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	SH2	e3cblA3	A: a+b two layers	X: SH2 (From Topology)	H: SH2 (From Topology)	T: SH2	F: SH2	ECOD (1.6)
A	Pkinase_Tyr	e3cblA1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.505.10	Alpha Beta	2-Layer Sandwich	SHC Adaptor Protein	SH2 domain	CATH (4.3.0)
A	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
A	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00017	SH2 domain (SH2)	SH2 domain	-	Domain
A	PF07714	Protein tyrosine and serine/threonine kinase (PK_Tyr_Ser-Thr)	Protein tyrosine and serine/threonine kinase	Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosph ...	Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function. Phosphoprotein phosphatases catalyse the reverse process. Protein kinases fall into three broad classes, characterised with respect to substrate specificity [1]; Serine/threonine-protein kinases, tyrosine-protein kinases, and dual specificity protein kinases (e.g. MEK - phosphorylates both Thr and Tyr on target proteins). This entry represents the catalytic domain found in a number of serine/threonine- and tyrosine-protein kinases. It does not include the catalytic domain of dual specificity kinases.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B	Synthetic peptide	-	-	-
A	Proto-oncogene tyrosine-protein kinase Fes/Fps	binding phospholipid binding lipid binding phosphatidylinositol binding protein binding signaling receptor binding immunoglobulin receptor binding protein tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor binding phospholipid binding lipid binding phosphatidylinositol binding protein binding signaling receptor binding immunoglobulin receptor binding protein tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding cytoskeletal protein binding microtubule binding tubulin binding non-membrane spanning protein tyrosine kinase activity	regulation of biological process cell adhesion regulation of cellular process regulation of cell adhesion biological regulation cellular process cellular component organization microtubule bundle formation organelle organization microtubule cytoskeleton organization microtubule-based process cellular component organization or biogenesis cytoskeleton organization myoblast proliferation regulation of biological process cell adhesion regulation of cellular process regulation of cell adhesion biological regulation cellular process cellular component organization microtubule bundle formation organelle organization microtubule cytoskeleton organization microtubule-based process cellular component organization or biogenesis cytoskeleton organization myoblast proliferation cell population proliferation developmental process heart development multicellular organismal process heart growth developmental growth cardiac muscle tissue development cardiac muscle cell proliferation animal organ development growth organ growth tissue development striated muscle tissue development circulatory system development multicellular organism development anatomical structure development muscle cell proliferation cardiac muscle tissue growth muscle tissue development striated muscle cell proliferation system development cellular developmental process regulation of developmental process positive regulation of biological process cell development regulation of cell differentiation regulation of cell development positive regulation of developmental process positive regulation of cell differentiation cell differentiation regulation of immune system process regulation of multicellular organismal process positive regulation of cellular process regulation of multicellular organismal development hemopoiesis regulation of myeloid cell differentiation myeloid cell differentiation immune system process positive regulation of myeloid cell differentiation regulation of hemopoiesis protein phosphorylation protein autophosphorylation phosphorylation cellular metabolic process primary metabolic process macromolecule modification phosphate-containing compound metabolic process metabolic process protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process cellular response to stimulus response to nutrient levels response to stimulus response to lipid response to nutrient cellular response to organic cyclic compound response to oxygen-containing compound response to vitamin cellular response to oxygen-containing compound cellular response to vitamin D cellular response to nutrient levels cellular response to chemical stimulus response to vitamin D cellular response to vitamin response to chemical cellular response to lipid cellular response to nutrient response to organic cyclic compound regulation of localization regulation of transport transport regulation of vesicle-mediated transport establishment of localization vesicle-mediated transport localization regulation of neuron projection development regulation of cellular component organization positive regulation of cellular component organization cell projection organization neuron projection development neurogenesis neuron differentiation positive regulation of neuron projection development positive regulation of cell projection organization plasma membrane bounded cell projection organization regulation of cell projection organization nervous system development generation of neurons neuron development regulation of plasma membrane bounded cell projection organization regulation of cell shape cell morphogenesis regulation of cell morphogenesis anatomical structure morphogenesis regulation of biological quality regulation of anatomical structure morphogenesis chemotaxis taxis response to external stimulus locomotion peptidyl-tyrosine modification peptidyl-amino acid modification peptidyl-tyrosine phosphorylation microtubule organizing center organization centrosome cycle cell cycle cell cycle process myeloid leukocyte differentiation regulation of leukocyte differentiation positive regulation of monocyte differentiation mononuclear cell differentiation positive regulation of hemopoiesis monocyte differentiation positive regulation of immune system process regulation of monocyte differentiation positive regulation of cell development regulation of myeloid leukocyte differentiation positive regulation of myeloid leukocyte differentiation positive regulation of multicellular organismal process positive regulation of leukocyte differentiation leukocyte differentiation positive regulation of organelle organization regulation of cytoskeleton organization regulation of microtubule polymerization or depolymerization cellular component biogenesis regulation of protein-containing complex assembly positive regulation of protein-containing complex assembly cellular component assembly microtubule polymerization or depolymerization positive regulation of protein polymerization protein-containing complex assembly protein polymerization positive regulation of microtubule polymerization or depolymerization protein-containing complex organization positive regulation of cellular component biogenesis positive regulation of cytoskeleton organization regulation of microtubule-based process microtubule polymerization positive regulation of supramolecular fiber organization regulation of cellular component biogenesis regulation of organelle organization positive regulation of microtubule polymerization regulation of protein polymerization regulation of microtubule polymerization regulation of supramolecular fiber organization supramolecular fiber organization regulation of microtubule cytoskeleton organization regulation of cell population proliferation regulation of cell motility regulation of locomotion cell motility establishment of organelle localization regulation of immune effector process regulation of myeloid leukocyte mediated immunity regulated exocytosis cell activation involved in immune response leukocyte degranulation myeloid leukocyte mediated immunity myeloid leukocyte activation establishment of localization in cell leukocyte mediated immunity immune response vacuolar localization mast cell degranulation immune effector process regulation of secretion regulation of leukocyte mediated immunity exocytosis lysosome localization cellular localization regulation of mast cell degranulation leukocyte activation regulation of regulated secretory pathway secretion by cell regulation of secretion by cell organelle localization leukocyte activation involved in immune response mast cell activation regulation of leukocyte degranulation mast cell mediated immunity secretion cell activation export from cell myeloid cell activation involved in immune response mast cell activation involved in immune response regulation of exocytosis	anchoring junction cell junction cellular anatomical entity focal adhesion cell-substrate junction intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane membrane intracellular organelle cytoskeleton organelle anchoring junction cell junction cellular anatomical entity focal adhesion cell-substrate junction intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane membrane intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle microtubule cytoskeleton non-membrane-bounded organelle membrane-bounded organelle cytoplasmic vesicle intracellular vesicle intracellular membrane-bounded organelle vesicle Golgi apparatus endomembrane system cytoplasmic side of plasma membrane cytoplasmic side of membrane side of membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR017441	Protein kinase, ATP binding site	Binding Site
A	IPR036860	SH2 domain superfamily	Homologous Superfamily
A	IPR008266	Tyrosine-protein kinase, active site	Active Site
A	IPR020635	Tyrosine-protein kinase, catalytic domain	Domain
A	IPR000980	SH2 domain	Domain
A	IPR000719	Protein kinase domain	Domain
A	IPR027267	AH/BAR domain superfamily	Homologous Superfamily
A	IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	Domain
A	IPR031160	F-BAR domain	Domain
A	IPR016250	Tyrosine-protein kinase, Fes/Fps type	Family
A	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A	IPR050198	Non-receptor tyrosine kinases involved in cell signaling	Family
A	IPR001060	FCH domain	Domain
A	IPR035849	Fes/Fps/Fer, SH2 domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P07332	ALK fusion gene-positive non-small-cell lung cancer bipolar disorder endogenous depression myocardial infarction tuberculosis attention deficit hyperactivity disorder, inattentive type breast carcinoma heart disease non-small cell lung carcinoma autism spectrum disorder cancer hypertensive disorder osteosarcoma

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
B	ACE	RESID: AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354 PSI-MOD : N-acetyl-L-alanine MOD:00050 , N-acetyl-L-aspartic acid MOD:00051 , N-acetyl-L-cysteine MOD:00052 , N-acetyl-S-archeol-cysteine MOD:00897 , N-acetyl-L-glutamic acid MOD:00053 , N-acetyl-L-glutamine MOD:00054 , N-acetylglycine MOD:00055 , N-acetyl-L-methionine MOD:00058 , N-acetyl-L-proline MOD:00059 , N-acetyl-L-serine MOD:00060 , N,O-diacetylated L-serine MOD:00648 , N-acetyl-L-threonine MOD:00061 , N-acetyl-L-tyrosine MOD:00062 , N-acetyl-L-valine MOD:00063 , N2-acetyl-L-arginine MOD:00359

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.