3MN5

Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3mn5a2	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	Actin/HSP70	automated matches	(Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)
A	d3mn5a1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	automated matches	automated matches	(Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Actin-like ATPases	8040509	3000092	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Actin-like ATPases	8040506	3000092	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Actin_1st	e3mn5A1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_1st	ECOD (1.6)
A	Actin_2nd	e3mn5A2	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_2nd	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A	3.90.640.10	Alpha Beta	Alpha-Beta Complex	Actin	Chain A, domain 4	CATH (4.3.0)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Actin, alpha skeletal muscle	cytoskeletal protein binding titin binding protein binding binding cation binding ion binding calcium ion binding metal ion binding small molecule binding protein domain specific binding hydrolase activity catalytic activity troponin I binding cytoskeletal motor activator activity cytoskeletal protein binding titin binding protein binding binding cation binding ion binding calcium ion binding metal ion binding small molecule binding protein domain specific binding hydrolase activity catalytic activity troponin I binding cytoskeletal motor activator activity cytoskeletal motor regulator activity molecular function activator activity molecular function regulator activity magnesium ion binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding myosin heavy chain binding myosin binding tropomyosin binding calcium-dependent protein binding identical protein binding actin monomer binding actin binding	developmental process mesenchyme migration multicellular organismal process mesenchyme morphogenesis animal organ development mesenchyme development tissue development tissue morphogenesis anatomical structure morphogenesis tissue migration anatomical structure development animal organ morphogenesis myotube cell development skeletal muscle fiber development developmental process mesenchyme migration multicellular organismal process mesenchyme morphogenesis animal organ development mesenchyme development tissue development tissue morphogenesis anatomical structure morphogenesis tissue migration anatomical structure development animal organ morphogenesis myotube cell development skeletal muscle fiber development cellular developmental process skeletal muscle tissue development cell development muscle cell differentiation myotube differentiation striated muscle tissue development muscle structure development skeletal muscle organ development striated muscle cell development muscle cell development cell differentiation cellular process muscle tissue development muscle organ development striated muscle cell differentiation regulation of biosynthetic process regulation of biological process regulation of cellular process biological regulation positive regulation of biological process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process positive regulation of macromolecule biosynthetic process regulation of gene expression positive regulation of metabolic process biosynthetic process positive regulation of gene expression positive regulation of biosynthetic process regulation of macromolecule biosynthetic process metabolic process positive regulation of macromolecule metabolic process macromolecule metabolic process regulation of metabolic process positive regulation of cellular process cellular component assembly involved in morphogenesis skeletal myofibril assembly membraneless organelle assembly cellular component organization cellular component biogenesis cellular anatomical entity morphogenesis cellular component assembly actin filament-based process cellular component organization or biogenesis skeletal muscle thin filament assembly actin cytoskeleton organization actomyosin structure organization organelle assembly actin filament organization organelle organization myofibril assembly anatomical structure formation involved in morphogenesis supramolecular fiber organization cytoskeleton organization protein polymerization actin polymerization or depolymerization actin filament polymerization protein-containing complex organization protein-containing complex assembly actin filament bundle organization actin filament bundle assembly	intracellular anatomical structure skeletal muscle myofibril intracellular membraneless organelle intracellular organelle cellular anatomical structure organelle membraneless organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex cell projection intracellular anatomical structure skeletal muscle myofibril intracellular membraneless organelle intracellular organelle cellular anatomical structure organelle membraneless organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex cell projection cell leading edge plasma membrane bounded cell projection lamellipodium cytoskeleton actin filament bundle actin cytoskeleton actomyosin stress fiber contractile actin filament bundle polymeric cytoskeletal fiber actin filament protein-containing complex filamentous actin cell body striated muscle thin filament myofilament sarcomere actin-based cell projection filopodium
B [auth S]	Protein spire	cytoskeletal protein binding microtubule binding protein binding binding tubulin binding actin binding	establishment of organelle localization cellular localization sexual reproduction reproductive process meiotic cell cycle microtubule cytoskeleton organization establishment of meiotic spindle localization spindle localization meiotic cell cycle process establishment of localization cellular component organization localization organelle organization establishment of spindle localization organelle localization establishment of organelle localization cellular localization sexual reproduction reproductive process meiotic cell cycle microtubule cytoskeleton organization establishment of meiotic spindle localization spindle localization meiotic cell cycle process establishment of localization cellular component organization localization organelle organization establishment of spindle localization organelle localization establishment of localization in cell cellular process microtubule-based process cell cycle cellular component organization or biogenesis cytoskeleton organization cell cycle process membrane organization cytoskeleton-dependent cytokinesis cytokinesis cleavage furrow formation cytokinetic process membrane invagination cell division plasma membrane invagination developmental process cellular developmental process oogenesis multicellular organismal reproductive process cell development cellular process involved in reproduction in multicellular organism female gamete generation developmental process involved in reproduction gamete generation cell differentiation anatomical structure development germ cell development organelle fission meiotic cytokinesis nuclear division female meiotic nuclear division polar body extrusion after meiotic divisions meiotic nuclear division karyosome formation chromosome organization oocyte karyosome formation chromosome organization involved in meiotic cell cycle meiosis I cell cycle process actin cytoskeleton organization actin filament organization actin filament-based process actin nucleation supramolecular fiber organization protein localization establishment of protein localization nitrogen compound transport transport protein transport cellular macromolecule localization macromolecule localization protein polymerization actin polymerization or depolymerization actin filament polymerization protein-containing complex organization cellular component biogenesis cellular component assembly protein-containing complex assembly multicellular organismal process blastoderm segmentation anterior/posterior pattern specification anterior/posterior axis specification, embryo segmentation pattern specification process embryo development oocyte development tripartite regional subdivision embryonic axis specification multicellular organism development anterior/posterior axis specification cell maturation embryonic pattern specification oocyte axis specification oocyte construction oocyte anterior/posterior axis specification developmental maturation cytoplasm organization regionalization pole plasm assembly anatomical structure maturation maternal determination of anterior/posterior axis, embryo oocyte differentiation axis specification Golgi vesicle transport vesicle-mediated transport RNA localization pole plasm RNA localization regulation of cytoskeleton organization regulation of biological process regulation of cellular component organization regulation of cellular process biological regulation regulation of organelle organization pole plasm oskar mRNA localization pole plasm mRNA localization intracellular mRNA localization involved in anterior/posterior axis specification intracellular mRNA localization intracellular mRNA localization involved in pattern specification process intracellular transport epithelial cell differentiation follicle cell of egg chamber development epithelium development tissue development anatomical structure morphogenesis eggshell formation chorion-containing eggshell formation epithelial cell development anatomical structure formation involved in morphogenesis actin filament network formation	cell periphery cell cortex cellular anatomical structure intracellular anatomical structure cytoplasm plasma membrane membrane membrane-bounded organelle intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle membrane vesicle membrane cytoplasmic vesicle cell periphery cell cortex cellular anatomical structure intracellular anatomical structure cytoplasm plasma membrane membrane membrane-bounded organelle intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle membrane vesicle membrane cytoplasmic vesicle organelle vesicle organelle membrane cytoskeleton membraneless organelle intracellular membraneless organelle perinuclear region of cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR020902	Actin/actin-like conserved site	Conserved Site
A	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
A	IPR004001	Actin, conserved site	Conserved Site
A	IPR004000	Actin family	Family
B [auth S]	IPR011011	Zinc finger, FYVE/PHD-type	Homologous Superfamily
B [auth S]	IPR029901	Protein Spire	Family
B [auth S]	IPR003124	WH2 domain	Domain
B [auth S]	IPR013083	Zinc finger, RING/FYVE/PHD-type	Homologous Superfamily
B [auth S]	IPR011019	KIND domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.