3SSN

MycE Methyltransferase from the Mycinamycin Biosynthetic Pathway in Complex with Mg, SAH, and Mycinamycin VI

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	EUF08311	e3ssnA1	A: a+b two layers	X: Sterol carrier protein-like	H: Sterol carrier protein, SCP-related (From Topology)	T: Sterol carrier protein, SCP-related	F: EUF08311	ECOD (1.6)
A	Methyltransf_24	e3ssnA2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_24	ECOD (1.6)
B	EUF08311	e3ssnB2	A: a+b two layers	X: Sterol carrier protein-like	H: Sterol carrier protein, SCP-related (From Topology)	T: Sterol carrier protein, SCP-related	F: EUF08311	ECOD (1.6)
B	Methyltransf_24	e3ssnB1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_24	ECOD (1.6)
C	EUF08311	e3ssnC1	A: a+b two layers	X: Sterol carrier protein-like	H: Sterol carrier protein, SCP-related (From Topology)	T: Sterol carrier protein, SCP-related	F: EUF08311	ECOD (1.6)
C	Methyltransf_24	e3ssnC2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_24	ECOD (1.6)
D	EUF08311	e3ssnD1	A: a+b two layers	X: Sterol carrier protein-like	H: Sterol carrier protein, SCP-related (From Topology)	T: Sterol carrier protein, SCP-related	F: EUF08311	ECOD (1.6)
D	Methyltransf_24	e3ssnD2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_24	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.1050.30	Alpha Beta	2-Layer Sandwich	Nonspecific Lipid-transfer Protein	Chain A	CATH (4.3.0)
A	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
B	3.30.1050.30	Alpha Beta	2-Layer Sandwich	Nonspecific Lipid-transfer Protein	Chain A	CATH (4.3.0)
B	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
C	3.30.1050.30	Alpha Beta	2-Layer Sandwich	Nonspecific Lipid-transfer Protein	Chain A	CATH (4.3.0)
C	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
D	3.30.1050.30	Alpha Beta	2-Layer Sandwich	Nonspecific Lipid-transfer Protein	Chain A	CATH (4.3.0)
D	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF17843	MycE methyltransferase N-terminal (MycE_N)	MycE methyltransferase N-terminal	This is the N-terminal domain found in MycE from the mycinamicin biosynthetic pathway. MycE is a tetramer of a two-domain polypeptide, comprising a C-terminal catalytic MT domain and an N-terminal auxiliary domain, which is important for quaternary ...	This is the N-terminal domain found in MycE from the mycinamicin biosynthetic pathway. MycE is a tetramer of a two-domain polypeptide, comprising a C-terminal catalytic MT domain and an N-terminal auxiliary domain, which is important for quaternary assembly and for substrate binding [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Methyltransferase	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding O-methyltransferase activity transferase activity, transferring one-carbon groups transferase activity methyltransferase activity catalytic activity mycinamicin VI 2''-O-methyltransferase activity	methylation metabolic process protein-containing complex organization protein homotetramerization cellular component organization cellular component biogenesis cellular component assembly protein complex oligomerization protein tetramerization protein-containing complex assembly protein homooligomerization cellular process cellular component organization or biogenesis antibiotic metabolic process methylation metabolic process protein-containing complex organization protein homotetramerization cellular component organization cellular component biogenesis cellular component assembly protein complex oligomerization protein tetramerization protein-containing complex assembly protein homooligomerization cellular process cellular component organization or biogenesis antibiotic metabolic process biosynthetic process antibiotic biosynthetic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR040800	Methyltransferase MycE, N-terminal	Domain
A, B, C, D	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.