Annotations: 3T4S

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	CHASE domain-like	8045159	4004329	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Sensory domain-like	8057003	3001802	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Sensory domain-like	8057003	3001802	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	CHASE	e3t4sA1	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: CHASE	ECOD (1.6)
A	EUF08492	e3t4sA2	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: EUF08492	ECOD (1.6)
B	CHASE	e3t4sB1	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: CHASE	ECOD (1.6)
B	EUF08492	e3t4sB2	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: EUF08492	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	6.10.250.1190	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
A	3.30.450.350	Alpha Beta	2-Layer Sandwich	Beta-Lactamase	CHASE domain	CATH (4.3.0)
B	6.10.250.1190	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
B	3.30.450.350	Alpha Beta	2-Layer Sandwich	Beta-Lactamase	CHASE domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF03924	CHASE domain (CHASE)	CHASE domain	This domain is found in the extracellular portion of receptor-like proteins - such as serine/threonine kinases and adenylyl cyclases [1,2]. This is a ligand-binding domain that binds cytokinin (Matilla et.al., FEMS Microbiology Reviews, fuab043, 45, ...	This domain is found in the extracellular portion of receptor-like proteins - such as serine/threonine kinases and adenylyl cyclases [1,2]. This is a ligand-binding domain that binds cytokinin (Matilla et.al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Histidine kinase 4	phosphoric ester hydrolase activity phosphoprotein phosphatase activity catalytic activity, acting on a protein hydrolase activity, acting on ester bonds phosphatase activity hydrolase activity catalytic activity cytokinin receptor activity molecular transducer activity kinase activity protein kinase activity signaling receptor activity transferase activity phosphotransferase activity, nitrogenous group as acceptor phosphoric ester hydrolase activity phosphoprotein phosphatase activity catalytic activity, acting on a protein hydrolase activity, acting on ester bonds phosphatase activity hydrolase activity catalytic activity cytokinin receptor activity molecular transducer activity kinase activity protein kinase activity signaling receptor activity transferase activity phosphotransferase activity, nitrogenous group as acceptor transmembrane histidine kinase cytokinin receptor activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein histidine kinase activity transmembrane receptor histidine kinase activity transmembrane signaling receptor activity transmembrane receptor protein kinase activity molecular sensor activity phosphorelay sensor kinase activity molecular function regulator activity protein serine/threonine phosphatase activity myosin phosphatase activity protein binding binding kinase binding enzyme binding protein kinase binding protein histidine kinase binding	response to acid chemical response to stimulus response to stress response to chemical response to oxygen-containing compound response to abiotic stimulus response to water response to water deprivation developmental process regulation of biological process meristem development regulation of meristem development regulation of developmental process biological regulation response to acid chemical response to stimulus response to stress response to chemical response to oxygen-containing compound response to abiotic stimulus response to water response to water deprivation developmental process regulation of biological process meristem development regulation of meristem development regulation of developmental process biological regulation anatomical structure development tissue development multicellular organism development multicellular organismal process regulation of shoot system development shoot system development system development cellular response to stimulus regulation of cellular process signaling response to endogenous stimulus cellular response to cytokinin stimulus hormone-mediated signaling pathway cell communication cellular response to endogenous stimulus cytokinin-activated signaling pathway cellular response to chemical stimulus response to hormone response to cytokinin cellular process signal transduction cellular response to hormone stimulus biological process involved in interspecies interaction between organisms response to other organism defense response response to external stimulus response to biotic stimulus defense response to bacterium defense response to other organism response to bacterium response to external biotic stimulus chemical homeostasis homeostatic process carbohydrate homeostasis regulation of multicellular organismal development regulation of seedling development seed germination seedling development regulation of post-embryonic development regulation of seed germination post-embryonic development regulation of multicellular organismal process response to osmotic stress cellular response to chemical stress cellular response to osmotic stress intracellular signal transduction cellular response to environmental stimulus osmosensory signaling pathway cellular response to stress cellular response to abiotic stimulus phosphorelay signal transduction system protein phosphorylation phosphorylation cellular metabolic process primary metabolic process macromolecule modification phosphate-containing compound metabolic process metabolic process protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process response to disaccharide cellular response to oxygen-containing compound cellular response to sucrose stimulus cellular response to carbohydrate stimulus response to carbohydrate response to sucrose cellular response to disaccharide stimulus plant organ development root morphogenesis embryonic morphogenesis embryonic root morphogenesis embryo development plant organ morphogenesis anatomical structure morphogenesis root development root system development cellular response to nutrient levels response to nutrient levels cellular response to phosphate starvation response to starvation cellular response to starvation	cell periphery plasma membrane membrane cellular anatomical entity membrane-bounded organelle intracellular anatomical structure organelle subcompartment intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cell periphery plasma membrane membrane cellular anatomical entity membrane-bounded organelle intracellular anatomical structure organelle subcompartment intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR011006	CheY-like superfamily	Homologous Superfamily
A, B	IPR000836	Phosphoribosyltransferase domain	Domain
A, B	IPR036890	Histidine kinase/HSP90-like ATPase superfamily	Homologous Superfamily
A, B	IPR003661	Signal transduction histidine kinase, dimerisation/phosphoacceptor domain	Domain
A, B	IPR003594	Histidine kinase/HSP90-like ATPase domain	Domain
A, B	IPR004358	Signal transduction histidine kinase-related protein, C-terminal	Domain
A, B	IPR001789	Signal transduction response regulator, receiver domain	Domain
A, B	IPR036097	Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily	Homologous Superfamily
A, B	IPR050956	Two-component system histidine kinase	Family
A, B	IPR042240	CHASE domain superfamily	Homologous Superfamily
A, B	IPR005467	Histidine kinase domain	Domain
A, B	IPR006189	CHASE domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.