4EQL

Crystal Structure of GH3.12 in complex with AMP and salicylate

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	GH3_2nd	e4eqlA3	A: beta barrels	X: beta-barrel domain in acetyl-CoA synthetase-like proteins (From Topology)	H: beta-barrel domain in acetyl-CoA synthetase-like proteins (From Topology)	T: beta-barrel domain in acetyl-CoA synthetase-like proteins	F: GH3_2nd	ECOD (1.6)
A	GH3_3rd	e4eqlA2	A: a+b two layers	X: Alpha-lytic protease prodomain-like	H: a+b domain in acetyl-CoA synthetase-like proteins (From Topology)	T: a+b domain in acetyl-CoA synthetase-like proteins	F: GH3_3rd	ECOD (1.6)
A	GH3_1st	e4eqlA1	A: a/b three-layered sandwiches	X: Rossmann-like domain in Acetyl-CoA synthetase-like proteins (From Topology)	H: Rossmann-like domain in Acetyl-CoA synthetase-like proteins (From Topology)	T: Rossmann-like domain in Acetyl-CoA synthetase-like proteins	F: GH3_1st	ECOD (1.6)
B	GH3_2nd	e4eqlB2	A: beta barrels	X: beta-barrel domain in acetyl-CoA synthetase-like proteins (From Topology)	H: beta-barrel domain in acetyl-CoA synthetase-like proteins (From Topology)	T: beta-barrel domain in acetyl-CoA synthetase-like proteins	F: GH3_2nd	ECOD (1.6)
B	GH3_3rd	e4eqlB3	A: a+b two layers	X: Alpha-lytic protease prodomain-like	H: a+b domain in acetyl-CoA synthetase-like proteins (From Topology)	T: a+b domain in acetyl-CoA synthetase-like proteins	F: GH3_3rd	ECOD (1.6)
B	GH3_1st	e4eqlB1	A: a/b three-layered sandwiches	X: Rossmann-like domain in Acetyl-CoA synthetase-like proteins (From Topology)	H: Rossmann-like domain in Acetyl-CoA synthetase-like proteins (From Topology)	T: Rossmann-like domain in Acetyl-CoA synthetase-like proteins	F: GH3_1st	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF03321	GH3 auxin-responsive promoter (GH3)	GH3 auxin-responsive promoter	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	4-substituted benzoates-glutamate ligase GH3.12	acid-amino acid ligase activity catalytic activity ligase activity, forming carbon-nitrogen bonds N-vanillate-L-glutamate synthetase activity ligase activity N-(4-aminobenzoyl)-L-glutamate synthetase activity N-(4-hydroxybenzoyl)-L-glutamate synthetase activity N-benzoyl-L-glutamate synthetase activity	cellular response to stimulus cellular response to salicylic acid stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cellular response to organic cyclic compound response to oxygen-containing compound cellular response to oxygen-containing compound response to salicylic acid cell communication cellular response to chemical stimulus response to chemical cellular response to stimulus cellular response to salicylic acid stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cellular response to organic cyclic compound response to oxygen-containing compound cellular response to oxygen-containing compound response to salicylic acid cell communication cellular response to chemical stimulus response to chemical cellular process signal transduction salicylic acid mediated signaling pathway response to organic cyclic compound regulation of defense response regulation of response to biotic stimulus systemic acquired resistance biological process involved in interspecies interaction between organisms regulation of response to external stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus regulation of systemic acquired resistance regulation of response to stimulus defense response to other organism response to stress regulation of response to stress response to external biotic stimulus detection of external biotic stimulus detection of biotic stimulus detection of stimulus detection of other organism response to fungus detection of fungus response to decreased oxygen levels cellular response to oxygen levels cellular response to hypoxia cellular response to decreased oxygen levels cellular response to stress response to hypoxia response to oxygen levels response to abiotic stimulus immune response cell death symbiont-induced defense-related programmed cell death immune system process plant-type hypersensitive response innate immune response programmed cell death benzene-containing compound metabolic process oxoacid metabolic process benzoate metabolic process carboxylic acid metabolic process metabolic process small molecule metabolic process monocarboxylic acid metabolic process organic acid metabolic process defense response to bacterium response to bacterium regulation of plant-type hypersensitive response positive regulation of response to biotic stimulus regulation of cellular response to stress positive regulation of biological process regulation of programmed cell death positive regulation of response to stimulus positive regulation of response to external stimulus positive regulation of immune response regulation of immune system process positive regulation of immune system process positive regulation of cellular process positive regulation of programmed cell death positive regulation of innate immune response regulation of innate immune response positive regulation of defense response regulation of immune response positive regulation of plant-type hypersensitive response	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR004993	GH3 family	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.