Annotations: 4HI0

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
E	SCOP2 Family	CobW/HypB/UreG nucleotide-binding domain-like	8058223	4003988	SCOP2 (2022-06-29)
E	SCOP2 Superfamily	Simibi-like P-loop NTPases	8058224	3002018	SCOP2 (2022-06-29)
F	SCOP2B Superfamily	Simibi-like P-loop NTPases	8058224	3002018	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	UreF	e4hi0A1	A: alpha complex topology	X: Citrate synthase-like	H: Urease accessory protein ureF (From Topology)	T: Urease accessory protein ureF	F: UreF	ECOD (1.6)
C	UreF	e4hi0C1	A: alpha complex topology	X: Citrate synthase-like	H: Urease accessory protein ureF (From Topology)	T: Urease accessory protein ureF	F: UreF	ECOD (1.6)
B	UreD	e4hi0B1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Urease accessory protein ureH (From Topology)	T: Urease accessory protein ureH	F: UreD	ECOD (1.6)
D	UreD	e4hi0D1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Urease accessory protein ureH (From Topology)	T: Urease accessory protein ureH	F: UreD	ECOD (1.6)
E	PRK	e4hi0E2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PRK	ECOD (1.6)
E	MnmE_helical_2nd	e4hi0E1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: MnmE_helical_2nd	ECOD (1.6)
F	PRK	e4hi0F2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PRK	ECOD (1.6)
F	MnmE_helical_2nd	e4hi0F1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: MnmE_helical_2nd	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.4190.10	Mainly Alpha	Orthogonal Bundle	Urease accessory protein UreF	Urease accessory protein UreF	CATH (4.3.0)
C	1.10.4190.10	Mainly Alpha	Orthogonal Bundle	Urease accessory protein UreF	Urease accessory protein UreF	CATH (4.3.0)
E	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
F	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C	PF01730	UreF (UreF)	UreF	-	Family
B, D	PF01774	UreD urease accessory protein (UreD)	UreD urease accessory protein	-	Family
E, F	PF02492	CobW/HypB/UreG, nucleotide-binding domain (cobW)	CobW/HypB/UreG, nucleotide-binding domain	This domain is found in HypB, a hydrogenase expression / formation protein, and UreG a urease accessory protein. Both these proteins contain a P-loop nucleotide binding motif [2,3]. HypB has GTPase activity and is a guanine nucleotide binding prot ...	This domain is found in HypB, a hydrogenase expression / formation protein, and UreG a urease accessory protein. Both these proteins contain a P-loop nucleotide binding motif [2,3]. HypB has GTPase activity and is a guanine nucleotide binding protein [3]. It is not known whether UreG binds GTP or some other nucleotide. Both enzymes are involved in nickel binding. HypB can store nickel and is required for nickel dependent hydrogenase expression [1]. UreG is required for functional incorporation of the urease nickel metallocenter.[4] GTP hydrolysis may required by these proteins for nickel incorporation into other nickel proteins [1]. This family of domains also contains P47K (Swiss:P31521), a Pseudomonas chlororaphis protein needed for nitrile hydratase expression, and the cobW gene product (Swiss:P29937), which may be involved in cobalamin biosynthesis in Pseudomonas denitrificans [5].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C	Urease accessory protein UreF	cation binding transition metal ion binding ion binding binding nickel cation binding metal ion binding small molecule binding	metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity
B, D	Urease accessory protein UreH	cation binding transition metal ion binding ion binding binding nickel cation binding metal ion binding small molecule binding	-	intracellular anatomical structure cytoplasm cellular anatomical entity
E, F	Urease accessory protein UreG	cation binding transition metal ion binding ion binding binding nickel cation binding metal ion binding small molecule binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding cation binding transition metal ion binding ion binding binding nickel cation binding metal ion binding small molecule binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity identical protein binding protein binding	nitrogen cycle metabolic process catabolic process amide catabolic process small molecule metabolic process metabolic process urea catabolic process amide metabolic process small molecule catabolic process urea metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C	IPR002639	Urease accessory protein UreF	Family
A, C	IPR038277	UreF domain superfamily	Homologous Superfamily
B, D	IPR002669	Urease accessory protein UreD	Family
E, F	IPR004400	Urease accessory protein UreG	Family
E, F	IPR003495	CobW/HypB/UreG, nucleotide-binding domain	Domain
E, F	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.