4V2O

Structure of saposin B in complex with chloroquine

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Membrane Protein Annotation: OPM

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4v2oa_	All alpha proteins	Saposin-like	Saposin	Saposin B	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d4v2ob1	All alpha proteins	Saposin-like	Saposin	Saposin B	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d4v2ob2	Artifacts	Tags	Tags	Tags	N-terminal Tags	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d4v2oc1	All alpha proteins	Saposin-like	Saposin	Saposin B	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d4v2oc2	Artifacts	Tags	Tags	Tags	N-terminal Tags	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Saposin	8043548	3000601	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Saposin	8043548	3000601	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Saposin	8043548	3000601	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	SapB_2_1	e4v2oA1	A: alpha arrays	X: Saposin-like	H: Saposin-like (From Topology)	T: Saposin-like	F: SapB_2_1	ECOD (1.6)
B	SapB_2_1	e4v2oB1	A: alpha arrays	X: Saposin-like	H: Saposin-like (From Topology)	T: Saposin-like	F: SapB_2_1	ECOD (1.6)
C	SapB_2_1	e4v2oC1	A: alpha arrays	X: Saposin-like	H: Saposin-like (From Topology)	T: Saposin-like	F: SapB_2_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.225.10	Mainly Alpha	Orthogonal Bundle	NK-Lysin	Saposin-like	CATH (4.3.0)
B	1.10.225.10	Mainly Alpha	Orthogonal Bundle	NK-Lysin	Saposin-like	CATH (4.3.0)
C	1.10.225.10	Mainly Alpha	Orthogonal Bundle	NK-Lysin	Saposin-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF03489	Saposin-like type B, region 2 (SapB_2)	Saposin-like type B, region 2	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	SAPOSIN-B	protein binding binding enzyme binding protease binding amide binding ceramide binding organic acid binding sphingolipid binding glycolipid binding ganglioside GM3 binding glycosphingolipid binding ion binding ganglioside binding anion binding protein binding binding enzyme binding protease binding amide binding ceramide binding organic acid binding sphingolipid binding glycolipid binding ganglioside GM3 binding glycosphingolipid binding ion binding ganglioside binding anion binding lipid binding carboxylic acid binding carbohydrate derivative binding monocarboxylic acid binding small molecule binding scaffold protein binding phospholipid binding ganglioside GM1 binding ganglioside GT1b binding enzyme regulator activity enzyme activator activity molecular function activator activity molecular function regulator activity identical protein binding protein homodimerization activity protein dimerization activity ganglioside GM2 binding ganglioside GP1c binding	developmental process epithelial cell differentiation involved in prostate gland development multicellular organismal process epithelium development reproductive system development cellular developmental process prostate gland development reproductive process gland development animal organ development urogenital system development tissue development developmental process involved in reproduction epithelial cell differentiation multicellular organism development developmental process epithelial cell differentiation involved in prostate gland development multicellular organismal process epithelium development reproductive system development cellular developmental process prostate gland development reproductive process gland development animal organ development urogenital system development tissue development developmental process involved in reproduction epithelial cell differentiation multicellular organism development cell differentiation anatomical structure development reproductive structure development cellular process system development regulation of biological process regulation of cellular process regulation of lipid metabolic process biological regulation primary metabolic process metabolic process regulation of metabolic process lipid metabolic process regulation of primary metabolic process membrane lipid metabolic process sphingolipid metabolic process cellular localization organic anion transport nitrogen compound transport carboxylic acid transport transport glycolipid transport lipid localization lipid transport localization within membrane carbohydrate derivative transport establishment of localization organic acid transport localization ceramide transport amide transport macromolecule localization ganglioside GM1 transport to membrane establishment of localization in cell vacuolar transport intracellular transport lysosomal transport cellular response to stimulus response to stimulus signaling cell communication adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction positive regulation of molecular function regulation of molecular function positive regulation of hydrolase activity regulation of catalytic activity regulation of hydrolase activity positive regulation of beta-galactosidase activity positive regulation of catalytic activity catabolic process autophagy regulation of catabolic process regulation of autophagy process utilizing autophagic mechanism developmental growth growth organ growth prostate gland growth	membrane-bounded organelle intracellular anatomical structure lytic vacuole membrane-enclosed lumen vacuolar lumen vacuole intracellular membrane-bounded organelle lysosomal lumen intracellular organelle lumen intracellular organelle cellular anatomical structure organelle lumen organelle cytoplasm membrane-bounded organelle intracellular anatomical structure lytic vacuole membrane-enclosed lumen vacuolar lumen vacuole intracellular membrane-bounded organelle lysosomal lumen intracellular organelle lumen intracellular organelle cellular anatomical structure organelle lumen organelle cytoplasm lysosome extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle cell periphery extracellular matrix external encapsulating structure collagen-containing extracellular matrix intracellular vesicle endomembrane system bounding membrane of organelle vesicle membrane cytoplasmic vesicle vacuolar membrane membrane azurophil granule lytic vacuole membrane cytoplasmic vesicle membrane lysosomal membrane secretory granule primary lysosome secretory vesicle azurophil granule membrane organelle membrane secretory granule membrane plasma membrane endosome late endosome

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR011001	Saposin-like	Homologous Superfamily
A, B, C	IPR008373	Saposin	Family
A, B, C	IPR007856	Saposin-like type B, region 1	Domain
A, B, C	IPR021165	Saposin, chordata	Family
A, B, C	IPR003119	Saposin A-type domain	Domain
A, B, C	IPR008139	Saposin B type domain	Domain
A, B, C	IPR051428	Sphingolipid Activators and Surfactant Proteins	Family
A, B, C	IPR008138	Saposin B type, region 2	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C	Pharos: P07602	Krabbe disease, atypical, due to saposin A deficiency atypical Gaucher disease due to saposin C deficiency encephalopathy due to prosaposin deficiency metachromatic leukodystrophy due to saposin b deficiency metachromatic leukodystrophy leukodystrophy gangliosidosis Gaucher disease myopathy Parkinson disease sphingolipidosis astrocytic tumor brain disease inborn errors of metabolism Metachromatic Leukodystrophy, Infant Krabbe disease, atypical, due to saposin A deficiency atypical Gaucher disease due to saposin C deficiency encephalopathy due to prosaposin deficiency metachromatic leukodystrophy due to saposin b deficiency metachromatic leukodystrophy leukodystrophy gangliosidosis Gaucher disease myopathy Parkinson disease sphingolipidosis astrocytic tumor brain disease inborn errors of metabolism Metachromatic Leukodystrophy, Infant neurodegenerative disease plasma cell myeloma ovarian cancer psoriasis B-cell chronic lymphocytic leukemia IgA glomerulonephritis intellectual disability lysosomal storage disease

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C	OPM	Monotopic/peripheral	All alpha monotopic/peripheral	Saposin-like	Saposin

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.