5KY2

mouse POFUT1 in complex with O-glucosylated mouse Factor VII EGF1 and GDP

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d5ky2b_	Small proteins	Knottins (small inhibitors, toxins, lectins)	EGF/Laminin	EGF-type module	automated matches	Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2 Family	EGF-type module	8069542	4003198	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	EGF/Laminin-like	8069543	3000412	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	O-FucT_C	e5ky2A2	A: a/b three-layered sandwiches	X: UDP-Glycosyltransferase/glycogen phosphorylase (From Topology)	H: UDP-Glycosyltransferase/glycogen phosphorylase (From Topology)	T: UDP-Glycosyltransferase/glycogen phosphorylase	F: O-FucT_C	ECOD (1.6)
A	O-FucT_N	e5ky2A1	A: a/b three-layered sandwiches	X: UDP-Glycosyltransferase/glycogen phosphorylase (From Topology)	H: UDP-Glycosyltransferase/glycogen phosphorylase (From Topology)	T: UDP-Glycosyltransferase/glycogen phosphorylase	F: O-FucT_N	ECOD (1.6)
B	hEGF_3	e5ky2B1	A: few secondary structure elements	X: EGF-like	H: EGF-related	T: EGF/Laminin	F: hEGF_3	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.11340	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
A	3.40.50.11350	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
B	2.10.25.10	Mainly Beta	Ribbon	Laminin	Laminin	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF10250	GDP-fucose protein O-fucosyltransferase (O-FucT)	GDP-fucose protein O-fucosyltransferase	-	Family
B	PF00008	EGF-like domain (EGF)	EGF-like domain	There is no clear separation between noise and signal. Pfam:PF00053 is very similar, but has 8 instead of 6 conserved cysteines. Includes some cytokine receptors. The EGF domain misses the N-terminus regions of the Ca2+ binding EGF domains (this is t ...	There is no clear separation between noise and signal. Pfam:PF00053 is very similar, but has 8 instead of 6 conserved cysteines. Includes some cytokine receptors. The EGF domain misses the N-terminus regions of the Ca2+ binding EGF domains (this is the main reason of discrepancy between swiss-prot domain start/end and Pfam). The family is hard to model due to many similar but different sub-types of EGF domains. Pfam certainly misses a number of EGF domains.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	GDP-fucose protein O-fucosyltransferase 1	fucosyltransferase activity peptide-O-fucosyltransferase activity catalytic activity, acting on a protein glycosyltransferase activity transferase activity hexosyltransferase activity catalytic activity	monosaccharide metabolic process carbohydrate metabolic process hexose metabolic process small molecule metabolic process metabolic process primary metabolic process fucose metabolic process cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling monosaccharide metabolic process carbohydrate metabolic process hexose metabolic process small molecule metabolic process metabolic process primary metabolic process fucose metabolic process cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling biological regulation regulation of signal transduction cell communication Notch signaling pathway regulation of response to stimulus regulation of Notch signaling pathway regulation of cell communication cellular process signal transduction developmental process heart development multicellular organism development multicellular organismal process anatomical structure development animal organ development circulatory system development system development blood vessel morphogenesis tube development angiogenesis vasculature development anatomical structure morphogenesis tube morphogenesis anatomical structure formation involved in morphogenesis blood vessel development nervous system development somitogenesis epithelium development chordate embryonic development segmentation anterior/posterior pattern specification pattern specification process embryo development tissue development regionalization embryo development ending in birth or egg hatching somite development carbohydrate derivative biosynthetic process cellular biosynthetic process cellular metabolic process glycoprotein biosynthetic process carbohydrate derivative metabolic process glycoprotein metabolic process protein glycosylation macromolecule biosynthetic process biosynthetic process glycosylation macromolecule modification macromolecule glycosylation protein O-linked glycosylation protein metabolic process macromolecule metabolic process protein modification process protein O-linked fucosylation fucosylation	membrane cellular anatomical entity membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle endoplasmic reticulum cytoplasm intracellular membrane-bounded organelle endomembrane system
B	Coagulation factor VII	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding protein binding signaling receptor binding peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity serine-type endopeptidase activity cation binding ion binding binding calcium ion binding metal ion binding small molecule binding protein binding signaling receptor binding peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity serine-type endopeptidase activity catalytic activity endopeptidase activity	multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding rhythmic process circadian rhythm response to hormone multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding rhythmic process circadian rhythm response to hormone response to endogenous stimulus response to chemical response to estrogen response to lipid response to astaxanthin cellular response to stimulus positive regulation of cell communication regulation of cellular process positive regulation of signal transduction signaling positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction positive regulation of TOR signaling signal transduction positive regulation of cellular process TOR signaling regulation of biological process regulation of signaling regulation of TOR signaling positive regulation of intracellular signal transduction regulation of response to stimulus regulation of cell communication cellular process positive regulation of signaling response to sterol response to alcohol response to oxygen-containing compound response to cholesterol response to organic cyclic compound response to thyrotropin-releasing hormone response to peptide hormone response to nitrogen compound cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation biosynthetic process proteolysis protein processing metabolic process protein metabolic process macromolecule metabolic process response to carbon dioxide response to Thyroid stimulating hormone response to glycoprotein response to 2,3,7,8-tetrachlorodibenzodioxine response to thyroid hormone response to thyroxine response to amino acid response to L-phenylalanine derivative response to acid chemical positive chemotaxis chemotaxis positive regulation of locomotion regulation of response to external stimulus positive regulation of positive chemotaxis response to external stimulus regulation of chemotaxis positive regulation of chemotaxis regulation of positive chemotaxis positive regulation of response to external stimulus taxis regulation of locomotion locomotion response to growth hormone response to decreased oxygen levels response to hypoxia response to oxygen levels response to abiotic stimulus positive regulation of coagulation positive regulation of blood coagulation regulation of response to wounding regulation of coagulation regulation of response to stress regulation of multicellular organismal process positive regulation of hemostasis regulation of hemostasis positive regulation of response to wounding regulation of blood coagulation positive regulation of wound healing regulation of wound healing positive regulation of multicellular organismal process response to phenylpropanoid response to ketone response to hydroxyisoflavone response to genistein developmental process animal organ regeneration animal organ development anatomical structure development regeneration response to nutrient levels response to nutrient response to vitamin K response to vitamin regulation of cell motility positive regulation of cell migration positive regulation of cell motility regulation of cell migration cell migration regulation of immune system process cell motility positive regulation of immune system process cell chemotaxis regulation of leukocyte chemotaxis regulation of leukocyte migration positive regulation of leukocyte chemotaxis positive regulation of leukocyte migration leukocyte migration cellular response to chemical stimulus immune system process leukocyte chemotaxis regulation of platelet-derived growth factor receptor signaling pathway cell surface receptor protein tyrosine kinase signaling pathway enzyme-linked receptor protein signaling pathway positive regulation of platelet-derived growth factor receptor signaling pathway cell surface receptor signaling pathway platelet-derived growth factor receptor signaling pathway response to estradiol	membrane-bounded organelle organelle cellular anatomical entity vesicle extracellular region protein-containing complex catalytic complex peptidase complex serine-type peptidase complex extracellular space

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR039922	GDP-fucose protein O-fucosyltransferase 1	Family
A	IPR019378	GDP-fucose protein O-fucosyltransferase	Family
B	IPR000294	Gamma-carboxyglutamic acid-rich (GLA) domain	Domain
B	IPR018097	EGF-like calcium-binding, conserved site	Conserved Site
B	IPR000742	EGF-like domain	Domain
B	IPR018114	Serine proteases, trypsin family, histidine active site	Active Site
B	IPR050442	Peptidase S1 family, coagulation factors	Family
B	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
B	IPR017857	Coagulation factor-like, Gla domain superfamily	Homologous Superfamily
B	IPR001314	Peptidase S1A, chymotrypsin family	Family
B	IPR033116	Serine proteases, trypsin family, serine active site	Active Site
B	IPR001254	Serine proteases, trypsin domain	Domain
B	IPR035972	Gamma-carboxyglutamic acid-rich (GLA) domain superfamily	Homologous Superfamily
B	IPR001881	EGF-like calcium-binding domain	Domain
B	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily
B	IPR000152	EGF-type aspartate/asparagine hydroxylation site	PTM
B	IPR012224	Peptidase S1A, coagulation factor VII/IX/X/C/Z	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.