5WIE

Crystal structure of a Kv1.2-2.1 chimera K+ channel V406W mutant in an inactivated state

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	NAD(P)-linked oxidoreductase	8040991	3000288	SCOP2B (2022-06-29)
C [auth G]	SCOP2B Superfamily	NAD(P)-linked oxidoreductase	8040991	3000288	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	POZ domain	8035031	3000894	SCOP2B (2022-06-29)
D [auth H]	SCOP2B Superfamily	POZ domain	8035031	3000894	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Aldo_ket_red	e5wieA1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Aldo_ket_red	ECOD (1.6)
C [auth G]	Aldo_ket_red	e5wieG1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Aldo_ket_red	ECOD (1.6)
B	Ion_trans	e5wieB2	A: alpha complex topology	X: Voltage-gated ion channels (From Topology)	H: Voltage-gated ion channels (From Topology)	T: Voltage-gated ion channels	F: Ion_trans	ECOD (1.6)
B	BTB_2	e5wieB3	A: a+b two layers	X: POZ domain (From Topology)	H: POZ domain (From Topology)	T: POZ domain	F: BTB_2	ECOD (1.6)
D [auth H]	Ion_trans	e5wieH3	A: alpha complex topology	X: Voltage-gated ion channels (From Topology)	H: Voltage-gated ion channels (From Topology)	T: Voltage-gated ion channels	F: Ion_trans	ECOD (1.6)
D [auth H]	BTB_2	e5wieH2	A: a+b two layers	X: POZ domain (From Topology)	H: POZ domain (From Topology)	T: POZ domain	F: BTB_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.100	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	NADP-dependent oxidoreductase domain	CATH (4.3.0)
C [auth G]	3.20.20.100	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	NADP-dependent oxidoreductase domain	CATH (4.3.0)
B	3.30.710.10	Alpha Beta	2-Layer Sandwich	Potassium Channel Kv1.1	Chain A	CATH (4.3.0)
B	1.20.120.350	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Voltage-gated potassium channels. Chain C	CATH (4.3.0)
B	1.10.287.70	Mainly Alpha	Orthogonal Bundle	Helix Hairpins		CATH (4.3.0)
D [auth H]	3.30.710.10	Alpha Beta	2-Layer Sandwich	Potassium Channel Kv1.1	Chain A	CATH (4.3.0)
D [auth H]	1.20.120.350	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Voltage-gated potassium channels. Chain C	CATH (4.3.0)
D [auth H]	1.10.287.70	Mainly Alpha	Orthogonal Bundle	Helix Hairpins		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C [auth G]	PF00248	Aldo/keto reductase family (Aldo_ket_red)	Aldo/keto reductase family	This family includes a number of K+ ion channel beta chain regulatory domains - these are reported to have oxidoreductase activity [2].	Domain
B, D [auth H]	PF02214	BTB/POZ domain (BTB_2)	BTB/POZ domain	In voltage-gated K+ channels this domain is responsible for subfamily-specific assembly of alpha-subunits into functional tetrameric channels [1]. In KCTD1 (Swiss:Q719H9) this domain functions as a transcriptional repressor [2]. It also mediates homo ...	In voltage-gated K+ channels this domain is responsible for subfamily-specific assembly of alpha-subunits into functional tetrameric channels [1]. In KCTD1 (Swiss:Q719H9) this domain functions as a transcriptional repressor [2]. It also mediates homomultimerisation of KCTD1 and interaction of KCTD1 with the transcription factor AP-2-alpha [2-3].	Domain
B, D [auth H]	PF07885	Ion channel (Ion_trans_2)	Ion channel	-	Family
B, D [auth H]	PF00520	Ion transport protein (Ion_trans)	Ion transport protein	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C [auth G]	Voltage-gated potassium channel subunit beta-2	methylglyoxal reductase (NADPH) (acetol producing) activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors catalytic activity monoatomic cation transmembrane transporter activity gated channel activity monoatomic ion channel activity potassium ion transmembrane transporter activity voltage-gated channel activity voltage-gated monoatomic ion channel activity potassium channel activity voltage-gated monoatomic cation channel activity monoatomic cation channel activity methylglyoxal reductase (NADPH) (acetol producing) activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors catalytic activity monoatomic cation transmembrane transporter activity gated channel activity monoatomic ion channel activity potassium ion transmembrane transporter activity voltage-gated channel activity voltage-gated monoatomic ion channel activity potassium channel activity voltage-gated monoatomic cation channel activity monoatomic cation channel activity passive transmembrane transporter activity voltage-gated potassium channel activity inorganic molecular entity transmembrane transporter activity channel activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity metal ion transmembrane transporter activity transmembrane transporter activity protein binding binding transmembrane transporter binding aldo-keto reductase (NADPH) activity alcohol dehydrogenase [NAD(P)+] activity protein-containing complex binding potassium channel regulator activity channel regulator activity ion channel regulator activity molecular function regulator activity transporter regulator activity	developmental process muscle structure development cellular developmental process cell differentiation anatomical structure development cellular process myoblast differentiation protein localization regulation of localization cellular localization regulation of biological process regulation of cellular process regulation of protein localization biological regulation developmental process muscle structure development cellular developmental process cell differentiation anatomical structure development cellular process myoblast differentiation protein localization regulation of localization cellular localization regulation of biological process regulation of cellular process regulation of protein localization biological regulation localization protein localization to cell surface regulation of cellular localization cellular macromolecule localization macromolecule localization regulation of protein localization to cell surface regulation of action potential action potential regulation of biological quality regulation of membrane potential nucleoside phosphate metabolic process nicotinamide nucleotide metabolic process cellular metabolic process primary metabolic process nucleotide metabolic process purine-containing compound metabolic process NADP metabolic process nucleobase-containing compound metabolic process purine nucleotide metabolic process organophosphate metabolic process nucleobase-containing small molecule metabolic process phosphate-containing compound metabolic process pyridine-containing compound metabolic process metabolic process small molecule metabolic process NADPH oxidation phosphorus metabolic process pyridine nucleotide metabolic process multicellular organismal process system process nervous system process neuromuscular process regulation of potassium ion transport transport monoatomic ion transmembrane transport potassium ion transmembrane transport regulation of monoatomic ion transmembrane transport metal ion transport monoatomic cation transmembrane transport monoatomic ion transport monoatomic cation transport regulation of metal ion transport regulation of transport regulation of monoatomic ion transport inorganic ion transmembrane transport regulation of potassium ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport regulation of transmembrane transport potassium ion transport regulation of monoatomic cation transmembrane transport hemopoiesis cell development hematopoietic progenitor cell differentiation	postsynaptic specialization membrane postsynaptic specialization synapse membrane plasma membrane cellular anatomical entity postsynaptic density membrane synaptic membrane cell periphery postsynaptic density postsynapse neuron to neuron synapse organelle cell junction postsynaptic specialization membrane postsynaptic specialization synapse membrane plasma membrane cellular anatomical entity postsynaptic density membrane synaptic membrane cell periphery postsynaptic density postsynapse neuron to neuron synapse organelle cell junction postsynaptic membrane asymmetric synapse plasma membrane region axon cell projection main axon juxtaparanode region of axon neuron projection plasma membrane bounded cell projection intracellular anatomical structure cytoplasm cytosol axon initial segment glutamatergic synapse voltage-gated potassium channel complex protein-containing complex plasma membrane protein complex potassium channel complex membrane protein complex cation channel complex transporter complex transmembrane transporter complex monoatomic ion channel complex intracellular organelle cytoskeleton intracellular non-membrane-bounded organelle non-membrane-bounded organelle pinceau fiber axon terminus presynapse distal axon neuron projection terminus cytoplasmic side of plasma membrane cytoplasmic side of membrane side of membrane
B, D [auth H]	Potassium voltage-gated channel subfamily A member 2,Potassium voltage-gated channel subfamily A member 2	cytoskeletal protein binding protein binding binding kinesin binding passive transmembrane transporter activity gated channel activity voltage-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential monoatomic ion channel activity channel activity voltage-gated channel activity voltage-gated monoatomic ion channel activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity cytoskeletal protein binding protein binding binding kinesin binding passive transmembrane transporter activity gated channel activity voltage-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential monoatomic ion channel activity channel activity voltage-gated channel activity voltage-gated monoatomic ion channel activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity monoatomic cation transmembrane transporter activity potassium ion transmembrane transporter activity potassium channel activity voltage-gated monoatomic cation channel activity monoatomic cation channel activity voltage-gated potassium channel activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity metal ion transmembrane transporter activity delayed rectifier potassium channel activity voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential outward rectifier potassium channel activity	developmental process corpus callosum development multicellular organismal process head development forebrain development animal organ development brain development nervous system development multicellular organism development central nervous system development anatomical structure development telencephalon development system development nerve development developmental process corpus callosum development multicellular organismal process head development forebrain development animal organ development brain development nervous system development multicellular organism development central nervous system development anatomical structure development telencephalon development system development nerve development cranial nerve development optic nerve development transport monoatomic ion transmembrane transport potassium ion transmembrane transport inorganic ion transmembrane transport establishment of localization metal ion transport inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization monoatomic ion transport potassium ion transport cellular process monoatomic cation transport biological regulation action potential regulation of biological quality regulation of membrane potential monoamine transport regulation of localization nitrogen compound transport regulation of cellular process signaling regulation of catecholamine secretion organic hydroxy compound transport dopamine transport cell communication secretion by cell regulation of secretion by cell catecholamine transport signal release regulation of biological process secretion regulation of transport export from cell cell-cell signaling dopamine secretion regulation of secretion catecholamine secretion amine transport regulation of amine transport regulation of dopamine secretion cranial nerve morphogenesis anatomical structure morphogenesis cranial nerve structural organization anatomical structure arrangement optic nerve morphogenesis optic nerve structural organization export across plasma membrane potassium ion export across plasma membrane system process nervous system process transmission of nerve impulse neuronal action potential circadian sleep/wake cycle, sleep rhythmic process circadian sleep/wake cycle rhythmic behavior circadian rhythm regulation of circadian sleep/wake cycle regulation of circadian sleep/wake cycle, non-REM sleep regulation of circadian rhythm circadian sleep/wake cycle, non-REM sleep behavior regulation of behavior circadian behavior sleep regulation of multicellular organismal process regulation of circadian sleep/wake cycle, sleep circadian sleep/wake cycle process pallium development cerebral cortex development sensory perception sensory perception of pain cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein complex oligomerization protein-containing complex assembly protein homooligomerization cellular component organization or biogenesis	cell projection cell leading edge cellular anatomical entity plasma membrane bounded cell projection lamellipodium cell periphery postsynapse postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region synaptic membrane cell projection cell leading edge cellular anatomical entity plasma membrane bounded cell projection lamellipodium cell periphery postsynapse postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region synaptic membrane axon main axon juxtaparanode region of axon neuron projection somatodendritic compartment cell body perikaryon neuronal cell body cell-cell junction anchoring junction paranodal junction presynapse presynaptic membrane dendrite dendritic tree axon initial segment glutamatergic synapse voltage-gated potassium channel complex protein-containing complex plasma membrane protein complex potassium channel complex membrane protein complex cation channel complex transporter complex transmembrane transporter complex monoatomic ion channel complex membrane-bounded organelle intracellular anatomical structure organelle subcompartment intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum membrane leading edge membrane lamellipodium membrane cell projection membrane axon terminus distal axon calyx of Held neuron projection terminus endosome intracellular vesicle cytoplasmic vesicle vesicle neuronal cell body membrane cell body membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C [auth G]	IPR005399	Potassium channel, voltage-dependent, beta subunit, KCNAB-related	Family
A, C [auth G]	IPR036812	NADP-dependent oxidoreductase domain superfamily	Homologous Superfamily
A, C [auth G]	IPR005401	Potassium channel, voltage-dependent, beta subunit, KCNAB2	Family
A, C [auth G]	IPR023210	NADP-dependent oxidoreductase domain	Domain
A, C [auth G]	IPR005983	Potassium channel, voltage-dependent, beta subunit, KCNAB	Family
B, D [auth H]	IPR003972	Potassium channel, voltage dependent, Kv1	Family
B, D [auth H]	IPR000210	BTB/POZ domain	Domain
B, D [auth H]	IPR003968	Potassium channel, voltage dependent, Kv	Family
B, D [auth H]	IPR005821	Ion transport domain	Domain
B, D [auth H]	IPR003131	Potassium channel tetramerisation-type BTB domain	Domain
B, D [auth H]	IPR004049	Potassium channel, voltage dependent, Kv1.2	Family
B, D [auth H]	IPR011333	SKP1/BTB/POZ domain superfamily	Homologous Superfamily
B, D [auth H]	IPR027359	Voltage-dependent channel domain superfamily	Homologous Superfamily

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C [auth G], D [auth H]	OPM	Transmembrane	Alpha-helical polytopic	Ion channel (VIC) superfamily	KvAP voltage-regulated K channels

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C [auth G], D [auth H]	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B, C [auth G], D [auth H]	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C [auth G], D [auth H]	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Channels: Potassium, Sodium, & Proton Ion-Selective	Kv1.2/Kv2.1 Voltage-gated potassium channel chimera, V406W mutant

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.