6CIO

Pyruvate:ferredoxin oxidoreductase from Moorella thermoacetica with lactyl-TPP bound

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	pyruv_ox_red	e6cioA1	A: alpha bundles	X: Bromodomain-like	H: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase (From Topology)	T: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase	F: pyruv_ox_red	ECOD (1.6)
A	Fer4_21_1	e6cioA2	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
A	POR	e6cioA5	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: Pyruvate-ferredoxin oxidoreductase, PFOR, domain III	F: POR	ECOD (1.6)
A	Transketolase_C	e6cioA6	A: a/b three-layered sandwiches	X: TK C-terminal domain-like (From Topology)	H: TK C-terminal domain-like (From Topology)	T: TK C-terminal domain-like	F: Transketolase_C	ECOD (1.6)
A	POR_N	e6cioA4	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: POR_N	ECOD (1.6)
A	TPP_enzyme_C	e6cioA3	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: TPP_enzyme_C	ECOD (1.6)
B	pyruv_ox_red	e6cioB4	A: alpha bundles	X: Bromodomain-like	H: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase (From Topology)	T: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase	F: pyruv_ox_red	ECOD (1.6)
B	Fer4_21_1	e6cioB6	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
B	POR	e6cioB3	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: Pyruvate-ferredoxin oxidoreductase, PFOR, domain III	F: POR	ECOD (1.6)
B	Transketolase_C	e6cioB5	A: a/b three-layered sandwiches	X: TK C-terminal domain-like (From Topology)	H: TK C-terminal domain-like (From Topology)	T: TK C-terminal domain-like	F: Transketolase_C	ECOD (1.6)
B	POR_N	e6cioB2	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: POR_N	ECOD (1.6)
B	TPP_enzyme_C	e6cioB1	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: TPP_enzyme_C	ECOD (1.6)
C	pyruv_ox_red	e6cioC2	A: alpha bundles	X: Bromodomain-like	H: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase (From Topology)	T: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase	F: pyruv_ox_red	ECOD (1.6)
C	Fer4_21_1	e6cioC4	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
C	POR	e6cioC5	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: Pyruvate-ferredoxin oxidoreductase, PFOR, domain III	F: POR	ECOD (1.6)
C	Transketolase_C	e6cioC6	A: a/b three-layered sandwiches	X: TK C-terminal domain-like (From Topology)	H: TK C-terminal domain-like (From Topology)	T: TK C-terminal domain-like	F: Transketolase_C	ECOD (1.6)
C	POR_N	e6cioC3	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: POR_N	ECOD (1.6)
C	TPP_enzyme_C	e6cioC1	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: TPP_enzyme_C	ECOD (1.6)
D	pyruv_ox_red	e6cioD4	A: alpha bundles	X: Bromodomain-like	H: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase (From Topology)	T: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase	F: pyruv_ox_red	ECOD (1.6)
D	Fer4_21_1	e6cioD3	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
D	POR	e6cioD5	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: Pyruvate-ferredoxin oxidoreductase, PFOR, domain III	F: POR	ECOD (1.6)
D	Transketolase_C	e6cioD6	A: a/b three-layered sandwiches	X: TK C-terminal domain-like (From Topology)	H: TK C-terminal domain-like (From Topology)	T: TK C-terminal domain-like	F: Transketolase_C	ECOD (1.6)
D	POR_N	e6cioD2	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: POR_N	ECOD (1.6)
D	TPP_enzyme_C	e6cioD1	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: TPP_enzyme_C	ECOD (1.6)
E	pyruv_ox_red	e6cioE5	A: alpha bundles	X: Bromodomain-like	H: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase (From Topology)	T: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase	F: pyruv_ox_red	ECOD (1.6)
E	Fer4_21_1	e6cioE2	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
E	POR	e6cioE4	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: Pyruvate-ferredoxin oxidoreductase, PFOR, domain III	F: POR	ECOD (1.6)
E	Transketolase_C	e6cioE1	A: a/b three-layered sandwiches	X: TK C-terminal domain-like (From Topology)	H: TK C-terminal domain-like (From Topology)	T: TK C-terminal domain-like	F: Transketolase_C	ECOD (1.6)
E	POR_N	e6cioE3	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: POR_N	ECOD (1.6)
E	TPP_enzyme_C	e6cioE6	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: TPP_enzyme_C	ECOD (1.6)
F	pyruv_ox_red	e6cioF3	A: alpha bundles	X: Bromodomain-like	H: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase (From Topology)	T: Four-helical bundle insertion domain in pyruvate-ferredoxin oxidoreductase	F: pyruv_ox_red	ECOD (1.6)
F	Fer4_21_1	e6cioF2	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
F	POR	e6cioF6	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: Pyruvate-ferredoxin oxidoreductase, PFOR, domain III	F: POR	ECOD (1.6)
F	Transketolase_C	e6cioF1	A: a/b three-layered sandwiches	X: TK C-terminal domain-like (From Topology)	H: TK C-terminal domain-like (From Topology)	T: TK C-terminal domain-like	F: Transketolase_C	ECOD (1.6)
F	POR_N	e6cioF5	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: POR_N	ECOD (1.6)
F	TPP_enzyme_C	e6cioF4	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: TPP_enzyme_C	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F	PF01558	Pyruvate ferredoxin/flavodoxin oxidoreductase (POR)	Pyruvate ferredoxin/flavodoxin oxidoreductase	-	Family
A, B, C, D, E A, B, C, D, E, F	PF01855	Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg (POR_N)	Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg	-	Family
A, B, C, D, E A, B, C, D, E, F	PF10371	Domain of unknown function (EKR)	Domain of unknown function	EKR is a short, 33 residue, domain found in bacterial and some lower eukaryotic species which lies between a POR (pyruvate ferredoxin/flavodoxin oxidoreductase) Pfam:PF01558 and the 4Fe-4S binding domain Fer4 Pfam:PF00037. It contains a characteristi ...	EKR is a short, 33 residue, domain found in bacterial and some lower eukaryotic species which lies between a POR (pyruvate ferredoxin/flavodoxin oxidoreductase) Pfam:PF01558 and the 4Fe-4S binding domain Fer4 Pfam:PF00037. It contains a characteristic EKR sequence motif. The exact function of this domain is not known.	Domain
A, B, C, D, E A, B, C, D, E, F	PF17147	Pyruvate:ferredoxin oxidoreductase core domain II (PFOR_II)	Pyruvate:ferredoxin oxidoreductase core domain II	PFOR_II is a core domain of the anaerobic enzyme pyruvate:ferredoxin oxidoreductase and is necessary for inter subunit contacts in conjunction with domains I and IV [1,2].	Domain
A, B, C, D, E A, B, C, D, E, F	PF12838	4Fe-4S dicluster domain (Fer4_7)	4Fe-4S dicluster domain	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contai ...	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.	Domain
A, B, C, D, E A, B, C, D, E, F	PF02775	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (TPP_enzyme_C)	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F	PYRUVATE-FERREDOXIN OXIDOREDUCTASE	cation binding transition metal ion binding iron ion binding ion binding binding metal ion binding small molecule binding pyruvate synthase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors catalytic activity iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding cation binding transition metal ion binding iron ion binding ion binding binding metal ion binding small molecule binding pyruvate synthase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors catalytic activity iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding metal cluster binding vitamin binding thiamine pyrophosphate binding organic cyclic compound binding anion binding quaternary ammonium group binding heterocyclic compound binding sulfur compound binding	cellular metabolic process electron transport chain metabolic process cellular process generation of precursor metabolites and energy response to oxidative stress response to stimulus response to stress	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F	IPR011895	Pyruvate-flavodoxin oxidoreductase	Family
A, B, C, D, E A, B, C, D, E, F	IPR029061	Thiamin diphosphate-binding fold	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR009014	Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR019752	Pyruvate/ketoisovalerate oxidoreductase, catalytic domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR050722	Pyruvate:ferredoxin/flavodoxin oxidoreductase	Family
A, B, C, D, E A, B, C, D, E, F	IPR017900	4Fe-4S ferredoxin, iron-sulphur binding, conserved site	Conserved Site
A, B, C, D, E A, B, C, D, E, F	IPR002869	Pyruvate-flavodoxin oxidoreductase, central domain	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR033412	Pyruvate:ferredoxin oxidoreductase, core domain II	Domain
A, B, C, D, E A, B, C, D, E, F	IPR019456	Pyruvate-flavodoxin oxidoreductase, EKR domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR017896	4Fe-4S ferredoxin-type, iron-sulphur binding domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR037112	Pyruvate-flavodoxin oxidoreductase, EKR domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR002880	Pyruvate flavodoxin/ferredoxin oxidoreductase, pyrimidine binding domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR011766	Thiamine pyrophosphate enzyme, TPP-binding	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.

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6CIO

Pyruvate:ferredoxin oxidoreductase from Moorella thermoacetica with lactyl-TPP bound

External Resource: Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Protein Family Annotation Pfam Database Homepage

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

InterPro: Protein Family Classification InterPro Database Homepage