6ZSI

The mechanism of activation of the actin binding protein EHBP1 by Rab8 family members.

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d6zsia_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Rab8a	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d6zsib_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Rab8a	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8089844	3002022	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ras-like P-loop GTPases	8089844	3002022	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Ras	e6zsiA1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
B	Ras	e6zsiB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
C	DUF3585	e6zsiC1	A: alpha bundles	X: Long alpha-hairpin	H: bMERB domain (bivalent Mical/EHBP Rab binding) (From Topology)	T: bMERB domain (bivalent Mical/EHBP Rab binding)	F: DUF3585	ECOD (1.6)
D	DUF3585	e6zsiD1	A: alpha bundles	X: Long alpha-hairpin	H: bMERB domain (bivalent Mical/EHBP Rab binding) (From Topology)	T: bMERB domain (bivalent Mical/EHBP Rab binding)	F: DUF3585	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00071	Ras family (Ras)	Ras family	Includes sub-families Ras, Rab, Rac, Ral, Ran, Rap Ypt1 and more. Shares P-loop motif with GTP_EFTU, arf and myosin_head. See Pfam:PF00009 Pfam:PF00025, Pfam:PF00063. As regards Rab GTPases, these are important regulators of vesicle formation, motil ...	Includes sub-families Ras, Rab, Rac, Ral, Ran, Rap Ypt1 and more. Shares P-loop motif with GTP_EFTU, arf and myosin_head. See Pfam:PF00009 Pfam:PF00025, Pfam:PF00063. As regards Rab GTPases, these are important regulators of vesicle formation, motility and fusion. They share a fold in common with all Ras GTPases: this is a six-stranded beta-sheet surrounded by five alpha-helices [1].	Domain
C, D	PF12130	Bivalent Mical/EHBP Rab binding domain (bMERB_dom)	Bivalent Mical/EHBP Rab binding domain	A variety of different effector proteins interact specifically with GTP-bound Rab proteins and mediate their versatile roles in membrane trafficking, including budding of vesicles from a donor membrane, directed transport through the cell and finally ...	A variety of different effector proteins interact specifically with GTP-bound Rab proteins and mediate their versatile roles in membrane trafficking, including budding of vesicles from a donor membrane, directed transport through the cell and finally tethering and fusion with a target membrane. The 'bivalent Mical/EHBP Rab binding' (bMERB) domain is a Rab effector domain that is present in proteins of the Mical and EHBP families, both known to act in endosomal trafficking. The bMERB domain displays a preference for Rab8 family proteins (Rab8, 10, 13 and 15) and at least some of the bMERB domains contain two separate binding sites for Rab-proteins, allowing Micals and EHBPs to bind two Rabs simultaneously. The strong similarity between the two binding sites within one bMRB domain strongly suggests an evolutionarily development via duplication of a common ancestor supersecondary structure element. The bMERB domain has a completely alpha-helical fold consisting of a central helix and N- and C-terminal helices folding back on this central helix [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Ras-related protein Rab-8A	small GTPase binding protein binding binding enzyme binding GTPase binding protein tyrosine kinase binding kinase binding protein kinase binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides small GTPase binding protein binding binding enzyme binding GTPase binding protein tyrosine kinase binding kinase binding protein kinase binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding GDP binding cytoskeletal protein binding myosin V binding myosin binding	protein localization cellular localization establishment of protein localization nitrogen compound transport protein localization to synapse transport neurotransmitter receptor transport to postsynaptic membrane establishment of protein localization to postsynaptic membrane vesicle-mediated transport neurotransmitter receptor transport to plasma membrane localization cellular macromolecule localization regulation of biological quality protein localization to cell periphery protein localization cellular localization establishment of protein localization nitrogen compound transport protein localization to synapse transport neurotransmitter receptor transport to postsynaptic membrane establishment of protein localization to postsynaptic membrane vesicle-mediated transport neurotransmitter receptor transport to plasma membrane localization cellular macromolecule localization regulation of biological quality protein localization to cell periphery protein localization to postsynaptic membrane neurotransmitter receptor transport establishment of protein localization to membrane biological regulation localization within membrane protein localization to membrane establishment of localization protein-containing complex localization vesicle-mediated transport to the plasma membrane regulation of postsynaptic membrane neurotransmitter receptor levels protein transport macromolecule localization cellular process protein localization to postsynapse protein localization to cell junction receptor localization to synapse catabolic process autophagy metabolic process process utilizing autophagic mechanism protein localization to plasma membrane multicellular organismal process axonogenesis cellular developmental process cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development cell morphogenesis cellular component organization neurogenesis multicellular organism development cell projection morphogenesis neuron differentiation neuron projection morphogenesis cell differentiation anatomical structure development cellular component organization or biogenesis system development developmental process plasma membrane bounded cell projection organization nervous system development axon development cell morphogenesis involved in neuron differentiation generation of neurons anatomical structure morphogenesis neuron development regulation of localization regulation of biological process regulation of cellular process regulation of protein localization regulation of transport regulation of establishment of protein localization regulation of protein transport regulation of cellular localization regulation of neuronal synaptic plasticity regulation of synaptic plasticity trans-synaptic signaling regulation of signaling regulation of long-term neuronal synaptic plasticity modulation of chemical synaptic transmission signaling cell-cell signaling chemical synaptic transmission cell communication synaptic signaling regulation of trans-synaptic signaling anterograde trans-synaptic signaling regulation of cell communication vesicle-mediated transport in synapse secretion establishment of protein localization to extracellular region export from cell protein secretion protein localization to extracellular region secretion by cell protein localization to cilium protein localization to organelle membrane docking organelle localization by membrane tethering exocytosis organelle localization vesicle docking exocytic process vesicle docking involved in exocytosis membrane organization membrane fusion organelle membrane fusion vesicle organization organelle fusion organelle organization Golgi vesicle fusion to target membrane vesicle fusion Golgi vesicle transport organelle assembly plasma membrane bounded cell projection assembly cellular component biogenesis cellular component assembly cilium assembly cilium organization cell projection assembly cellular response to nitrogen compound cellular response to stimulus response to stimulus response to endogenous stimulus response to peptide hormone response to oxygen-containing compound cellular response to peptide hormone stimulus response to nitrogen compound cellular response to oxygen-containing compound cellular response to endogenous stimulus cellular response to chemical stimulus response to hormone response to chemical response to insulin cellular response to insulin stimulus cellular response to hormone stimulus regulation of catabolic process regulation of autophagy regulation of metabolic process intracellular transport endocytic recycling endosomal transport establishment of localization in cell establishment of protein localization to plasma membrane neurotransmitter receptor transport, endosome to plasma membrane neurotransmitter receptor transport, endosome to postsynaptic membrane endosome to plasma membrane protein transport intracellular protein transport Golgi organization endomembrane system organization	extracellular exosome membrane-bounded organelle extracellular vesicle cellular anatomical structure extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol synapse extracellular exosome membrane-bounded organelle extracellular vesicle cellular anatomical structure extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol synapse cell junction glutamatergic synapse membrane intracellular vesicle phagocytic vesicle intracellular membrane-bounded organelle intracellular organelle phagocytic vesicle membrane cytoplasmic vesicle membrane bounding membrane of organelle cytoplasmic vesicle vesicle membrane endocytic vesicle membrane organelle membrane endocytic vesicle Golgi membrane Golgi apparatus endomembrane system neuron spine neuron projection dendritic spine plasma membrane bounded cell projection somatodendritic compartment cell projection postsynapse dendrite dendritic tree endosome membrane endosome cell body neuronal cell body transport vesicle presynapse synaptic vesicle secretory vesicle exocytic vesicle intracellular membraneless organelle cilium ciliary basal body microtubule organizing center cytoskeleton membraneless organelle microtubule cytoskeleton midbody centrosome centriole cell periphery plasma membrane non-motile cilium ciliary membrane plasma membrane region cell projection membrane trans-Golgi network transport vesicle clathrin-coated vesicle coated vesicle Golgi-associated vesicle recycling endosome recycling endosome membrane postsynaptic density neuron to neuron synapse postsynaptic specialization asymmetric synapse Golgi apparatus subcompartment organelle subcompartment trans-Golgi network membrane trans-Golgi network ciliary base
C, D	EH domain-binding protein 1	-	localization import into cell transport cellular process endocytosis establishment of localization vesicle-mediated transport protein localization establishment of protein localization cellular localization nitrogen compound transport protein transport cellular macromolecule localization macromolecule localization	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane membrane membrane-bounded organelle endosome intracellular vesicle intracellular membrane-bounded organelle endomembrane system intracellular organelle cytoplasmic vesicle cellular anatomical structure intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane membrane membrane-bounded organelle endosome intracellular vesicle intracellular membrane-bounded organelle endomembrane system intracellular organelle cytoplasmic vesicle organelle vesicle nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B	IPR050305	Small GTPase superfamily, Rab	Family
A, B	IPR005225	Small GTP-binding domain	Domain
A, B	IPR001806	Small GTPase	Family
C, D	IPR036872	CH domain superfamily	Homologous Superfamily
C, D	IPR050540	F-actin Monooxygenase Mical	Family
C, D	IPR001715	Calponin homology domain	Domain
C, D	IPR019448	NT-type C2 domain	Domain
C, D	IPR022735	bMERB domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P61006	rheumatoid arthritis astrocytic tumor plasma cell myeloma cancer psoriasis ovarian cancer
C, D	Pharos: Q8NDI1	prostate cancer prostate cancer, hereditary, 12 psoriasis attention deficit hyperactivity disorder, inattentive type dermatomyositis Emery-Dreifuss muscular dystrophy invasive ductal breast carcinoma Malignant neoplasm of breast Pick disease prostate carcinoma sinusitis atypical teratoid rhabdoid tumor Duchenne muscular dystrophy glioblastoma juvenile dermatomyositis prostate cancer prostate cancer, hereditary, 12 psoriasis attention deficit hyperactivity disorder, inattentive type dermatomyositis Emery-Dreifuss muscular dystrophy invasive ductal breast carcinoma Malignant neoplasm of breast Pick disease prostate carcinoma sinusitis atypical teratoid rhabdoid tumor Duchenne muscular dystrophy glioblastoma juvenile dermatomyositis medulloblastoma tuberculosis breast carcinoma ductal breast carcinoma in situ heart disease large cell medulloblastoma ovarian cancer

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.