7AKO

Crystal structure of CHK1 kinase domain in complex with a CLASPIN phosphopeptide

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Protein kinase-like (PK-like)	8033807	3000066	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Protein kinase-like (PK-like)	8033807	3000066	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Pkinase_Tyr	e7akoA1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
B	Pkinase_Tyr	e7akoB1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00069	Protein kinase domain (Pkinase)	Protein kinase domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Serine/threonine-protein kinase Chk1	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein binding protein domain specific binding protein serine kinase activity protein serine/threonine kinase activity histone H3 kinase activity histone modifying activity histone H3T11 kinase activity histone kinase activity	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process regulation of DNA recombination regulation of cellular process regulation of double-strand break repair regulation of cellular response to stress regulation of primary metabolic process nucleobase-containing compound metabolic process regulation of response to stress recombinational repair cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process regulation of DNA recombination regulation of cellular process regulation of double-strand break repair regulation of cellular response to stress regulation of primary metabolic process nucleobase-containing compound metabolic process regulation of response to stress recombinational repair regulation of nucleobase-containing compound metabolic process double-strand break repair via homologous recombination DNA repair double-strand break repair regulation of biological process DNA recombination biological regulation primary metabolic process DNA metabolic process regulation of macromolecule metabolic process nucleic acid metabolic process regulation of DNA repair regulation of double-strand break repair via homologous recombination regulation of response to stimulus metabolic process macromolecule metabolic process regulation of metabolic process regulation of DNA metabolic process regulation of cell cycle process negative regulation of biological process negative regulation of cell cycle G0 to G1 transition negative regulation of cell cycle process regulation of G0 to G1 transition negative regulation of G0 to G1 transition negative regulation of cellular process cell cycle regulation of cell cycle cell cycle process replicative senescence cellular metabolic process apoptotic process cell death programmed cell death cellular response to nitrogen compound cellular response to caffeine cellular response to organic cyclic compound response to nitrogen compound response to purine-containing compound response to alkaloid cellular response to chemical stimulus response to chemical cellular response to alkaloid cellular response to purine-containing compound response to organic cyclic compound response to caffeine protein phosphorylation phosphorylation macromolecule modification peptidyl-amino acid modification phosphate-containing compound metabolic process peptidyl-threonine modification protein metabolic process protein modification process phosphorus metabolic process peptidyl-threonine phosphorylation developmental process multicellular organismal process inner cell mass cell proliferation cell population proliferation developmental growth chordate embryonic development growth embryo development in utero embryonic development multicellular organism development embryo development ending in birth or egg hatching anatomical structure development blastocyst growth blastocyst development cellular component organization nucleus organization organelle organization cellular component organization or biogenesis regulation of cell cycle phase transition signaling G2/M transition of mitotic cell cycle cell cycle phase transition intracellular signal transduction mitotic cell cycle negative regulation of mitotic cell cycle mitotic G2/M transition checkpoint cell communication regulation of mitotic cell cycle negative regulation of cell cycle phase transition signal transduction cell cycle G2/M phase transition negative regulation of mitotic cell cycle phase transition regulation of G2/M transition of mitotic cell cycle regulation of cell cycle G2/M phase transition cell cycle checkpoint signaling regulation of mitotic cell cycle phase transition mitotic cell cycle checkpoint signaling mitotic cell cycle process negative regulation of cell cycle G2/M phase transition negative regulation of G2/M transition of mitotic cell cycle mitotic cell cycle phase transition DNA damage checkpoint signaling DNA integrity checkpoint signaling signal transduction in response to DNA damage positive regulation of biological process positive regulation of cell cycle positive regulation of cellular process DNA replication apoptotic process involved in development cellular biosynthetic process regulation of DNA biosynthetic process negative regulation of cellular metabolic process nucleic acid biosynthetic process negative regulation of nucleobase-containing compound metabolic process negative regulation of macromolecule metabolic process regulation of biosynthetic process negative regulation of metabolic process regulation of cellular metabolic process nucleobase-containing compound biosynthetic process macromolecule biosynthetic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process negative regulation of DNA metabolic process regulation of macromolecule biosynthetic process DNA biosynthetic process negative regulation of DNA biosynthetic process chromatin organization chromatin remodeling epigenetic regulation of gene expression gene expression regulation of gene expression negative regulation of gene expression negative regulation of gene expression, epigenetic mitotic G2 DNA damage checkpoint signaling mitotic DNA integrity checkpoint signaling mitotic DNA damage checkpoint signaling response to mechanical stimulus response to external stimulus response to abiotic stimulus cellular response to mechanical stimulus cellular response to abiotic stimulus cellular response to external stimulus cellular response to environmental stimulus regulation of cell population proliferation regulation of signaling regulation of signal transduction signal transduction by p53 class mediator regulation of intracellular signal transduction regulation of signal transduction by p53 class mediator regulation of cell communication centrosome separation microtubule cytoskeleton organization mitotic centrosome separation centrosome cycle mitotic nuclear division microtubule organizing center organization organelle fission regulation of mitotic centrosome separation nuclear division microtubule-based process cytoskeleton organization regulation of cellular component organization regulation of mitotic nuclear division negative regulation of cellular component organization regulation of organelle organization negative regulation of nuclear division negative regulation of organelle organization regulation of nuclear division negative regulation of mitotic nuclear division	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle organelle intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle extracellular region extracellular space membrane-bounded organelle condensed nuclear chromosome intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle organelle intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle extracellular region extracellular space membrane-bounded organelle condensed nuclear chromosome nucleus membrane-enclosed lumen nuclear chromosome intracellular membrane-bounded organelle intracellular organelle lumen organelle lumen condensed chromosome nuclear lumen centrosome microtubule organizing center cytoskeleton microtubule cytoskeleton nucleoplasm protein-containing complex replication fork
C, D	Claspin	protein-containing complex binding anaphase-promoting complex binding binding DNA secondary structure binding DNA binding organic cyclic compound binding nucleic acid binding	cellular response to stimulus regulation of cellular process regulation of cell cycle phase transition response to stimulus signaling G2/M transition of mitotic cell cycle negative regulation of biological process negative regulation of cell cycle cell cycle phase transition intracellular signal transduction mitotic cell cycle negative regulation of mitotic cell cycle cell communication mitotic G2/M transition checkpoint regulation of mitotic cell cycle cellular response to stimulus regulation of cellular process regulation of cell cycle phase transition response to stimulus signaling G2/M transition of mitotic cell cycle negative regulation of biological process negative regulation of cell cycle cell cycle phase transition intracellular signal transduction mitotic cell cycle negative regulation of mitotic cell cycle cell communication mitotic G2/M transition checkpoint regulation of mitotic cell cycle mitotic DNA integrity checkpoint signaling negative regulation of cellular process negative regulation of cell cycle phase transition signal transduction cell cycle DNA replication checkpoint signaling regulation of cell cycle cell cycle process cell cycle G2/M phase transition regulation of biological process mitotic DNA replication checkpoint signaling negative regulation of mitotic cell cycle phase transition regulation of G2/M transition of mitotic cell cycle regulation of cell cycle process biological regulation DNA integrity checkpoint signaling regulation of cell cycle G2/M phase transition negative regulation of cell cycle process cell cycle checkpoint signaling regulation of mitotic cell cycle phase transition mitotic cell cycle checkpoint signaling mitotic cell cycle process negative regulation of cell cycle G2/M phase transition cellular process negative regulation of G2/M transition of mitotic cell cycle mitotic cell cycle phase transition DNA damage checkpoint signaling mitotic G2 DNA damage checkpoint signaling cellular response to stress response to stress mitotic DNA damage checkpoint signaling DNA damage response signal transduction in response to DNA damage primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process metabolic process macromolecule metabolic process DNA repair protein phosphorylation phosphorylation cellular metabolic process peptidyl-serine modification peptidyl-serine phosphorylation macromolecule modification peptidyl-amino acid modification phosphate-containing compound metabolic process protein metabolic process protein modification process phosphorus metabolic process positive regulation of phosphorylation regulation of catalytic activity regulation of transferase activity positive regulation of phosphate metabolic process regulation of primary metabolic process positive regulation of metabolic process regulation of phosphate metabolic process positive regulation of macromolecule metabolic process positive regulation of protein phosphorylation positive regulation of phosphorus metabolic process regulation of protein metabolic process regulation of cellular metabolic process regulation of molecular function positive regulation of kinase activity regulation of kinase activity activation of protein kinase activity regulation of phosphorus metabolic process regulation of metabolic process regulation of protein modification process regulation of protein phosphorylation positive regulation of protein metabolic process positive regulation of biological process positive regulation of cellular metabolic process positive regulation of molecular function positive regulation of protein modification process positive regulation of protein kinase activity positive regulation of cellular process positive regulation of transferase activity positive regulation of catalytic activity regulation of protein kinase activity regulation of macromolecule metabolic process regulation of phosphorylation	membrane-bounded organelle intracellular organelle intracellular anatomical structure Golgi apparatus organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle endomembrane system nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen membrane-bounded organelle intracellular organelle intracellular anatomical structure Golgi apparatus organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle endomembrane system nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR000719	Protein kinase domain	Domain
A, B	IPR017441	Protein kinase, ATP binding site	Binding Site
A, B	IPR034670	Checkpoint kinase 1, catalytic domain	Domain
A, B	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A, B	IPR008271	Serine/threonine-protein kinase, active site	Active Site
C, D	IPR024146	Claspin	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: O14757	breast cancer breast carcinoma polyploidy malignant mesothelioma malignant pancreatic neoplasm medulloblastoma ovarian cancer adrenal cortex carcinoma peripheral nervous system disease primitive neuroectodermal tumor cancer tuberculosis endometriosis IgA glomerulonephritis lung adenocarcinoma breast cancer breast carcinoma polyploidy malignant mesothelioma malignant pancreatic neoplasm medulloblastoma ovarian cancer adrenal cortex carcinoma peripheral nervous system disease primitive neuroectodermal tumor cancer tuberculosis endometriosis IgA glomerulonephritis lung adenocarcinoma malignant ependymoma Malignant neoplasm of breast nasopharyngeal carcinoma pancreatic intraductal papillary-mucinous carcinoma atopic eczema peripheral neuropathy psoriasis cystic fibrosis ulcerative colitis glioblastoma invasive ductal breast carcinoma lung cancer malignant glioma non-small cell lung carcinoma pancreatic intraductal papillary-mucinous neoplasm atypical teratoid rhabdoid tumor breast disease schizophrenia ductal breast carcinoma in situ hereditary breast carcinoma large cell medulloblastoma malignant colon neoplasm
C, D	Pharos: Q9HAW4	breast cancer Malignant neoplasm of breast psoriasis

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
C, D	SEP	Parent Component: SER RESID: AA0037 PSI-MOD : O-phospho-L-serine MOD:00046

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.