7M8R

Complex structure of Methane monooxygenase hydroxylase and regulatory subunit with fluorosubstituted tryptophans

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d7m8ra_	All alpha proteins	Ferritin-like	Ferritin-like	Ribonucleotide reductase-like	automated matches	(Methylosinus trichosporium OB3b ) [TaxId: 595536 ],	SCOPe (2.08)
E	d7m8re_	All alpha proteins	Ferritin-like	Ferritin-like	Ribonucleotide reductase-like	automated matches	(Methylosinus trichosporium OB3b ) [TaxId: 595536 ],	SCOPe (2.08)
B	d7m8rb_	All alpha proteins	Ferritin-like	Ferritin-like	Ribonucleotide reductase-like	automated matches	(Methylosinus trichosporium OB3b ) [TaxId: 595536 ],	SCOPe (2.08)
F	d7m8rf_	All alpha proteins	Ferritin-like	Ferritin-like	Ribonucleotide reductase-like	automated matches	(Methylosinus trichosporium OB3b ) [TaxId: 595536 ],	SCOPe (2.08)
C	d7m8rc_	All alpha proteins	Open three-helical up-and-down bundle	Methane monooxygenase hydrolase, gamma subunit	Methane monooxygenase hydrolase, gamma subunit	automated matches	(Methylosinus trichosporium OB3b ) [TaxId: 595536 ],	SCOPe (2.08)
G	d7m8rg_	All alpha proteins	Open three-helical up-and-down bundle	Methane monooxygenase hydrolase, gamma subunit	Methane monooxygenase hydrolase, gamma subunit	automated matches	(Methylosinus trichosporium OB3b ) [TaxId: 595536 ],	SCOPe (2.08)
D	d7m8rd_	Alpha and beta proteins (a+b)	Monooxygenase (hydroxylase) regulatory protein	Monooxygenase (hydroxylase) regulatory protein	Monooxygenase (hydroxylase) regulatory protein	automated matches	(Methylosinus trichosporium OB3b ) [TaxId: 595536 ],	SCOPe (2.08)
H	d7m8rh_	Alpha and beta proteins (a+b)	Monooxygenase (hydroxylase) regulatory protein	Monooxygenase (hydroxylase) regulatory protein	Monooxygenase (hydroxylase) regulatory protein	automated matches	(Methylosinus trichosporium OB3b ) [TaxId: 595536 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ferritin-like	8037031	3001658	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Ferritin-like	8037031	3001658	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ferritin-like	8096408	3001658	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Ferritin-like	8096408	3001658	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Methane monooxygenase hydrolase, gamma subunit	8096406	3001468	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Methane monooxygenase hydrolase, gamma subunit	8096406	3001468	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Monooxygenase (hydroxylase) regulatory protein	8096654	3000674	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Monooxygenase (hydroxylase) regulatory protein	8096654	3000674	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Phenol_Hydrox	e7m8rA1	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Ferritin/Heme oxygenase	T: Heme oxygenase/Ribonucleotide reductase	F: Phenol_Hydrox	ECOD (1.6)
E	Phenol_Hydrox	e7m8rE1	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Ferritin/Heme oxygenase	T: Heme oxygenase/Ribonucleotide reductase	F: Phenol_Hydrox	ECOD (1.6)
B	Phenol_Hydrox	e7m8rB1	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Ferritin/Heme oxygenase	T: Heme oxygenase/Ribonucleotide reductase	F: Phenol_Hydrox	ECOD (1.6)
F	Phenol_Hydrox	e7m8rF1	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Ferritin/Heme oxygenase	T: Heme oxygenase/Ribonucleotide reductase	F: Phenol_Hydrox	ECOD (1.6)
C	MeMO_Hyd_G	e7m8rC1	A: alpha arrays	X: Methane monooxygenase hydrolase gamma subunit-like	H: Methane monooxygenase hydrolase gamma subunit (From Topology)	T: Methane monooxygenase hydrolase gamma subunit	F: MeMO_Hyd_G	ECOD (1.6)
G	MeMO_Hyd_G	e7m8rG1	A: alpha arrays	X: Methane monooxygenase hydrolase gamma subunit-like	H: Methane monooxygenase hydrolase gamma subunit (From Topology)	T: Methane monooxygenase hydrolase gamma subunit	F: MeMO_Hyd_G	ECOD (1.6)
D	MmoB_DmpM	e7m8rD1	A: a+b two layers	X: Alpha-beta plaits	H: Monooxygenase (hydroxylase) regulatory protein (From Topology)	T: Monooxygenase (hydroxylase) regulatory protein	F: MmoB_DmpM	ECOD (1.6)
H	MmoB_DmpM	e7m8rH1	A: a+b two layers	X: Alpha-beta plaits	H: Monooxygenase (hydroxylase) regulatory protein (From Topology)	T: Monooxygenase (hydroxylase) regulatory protein	F: MmoB_DmpM	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, E	PF02332	Methane/Phenol/Alkene Hydroxylase (Phenol_Hydrox)	Methane/Phenol/Alkene Hydroxylase	This protein family includes several components of multicomponent enzyme systems predominantly found in Proteobacteria and Actinobacteria, including subunits alpha and beta of a methane monooxygenase and an alkene monooxygenase system, small and larg ...	This protein family includes several components of multicomponent enzyme systems predominantly found in Proteobacteria and Actinobacteria, including subunits alpha and beta of a methane monooxygenase and an alkene monooxygenase system, small and large subunits of propane 2-monooxygenase system and P1 protein of phenol hydroxylase [1-4].	Domain
B, F	PF02332	Methane/Phenol/Alkene Hydroxylase (Phenol_Hydrox)	Methane/Phenol/Alkene Hydroxylase	This protein family includes several components of multicomponent enzyme systems predominantly found in Proteobacteria and Actinobacteria, including subunits alpha and beta of a methane monooxygenase and an alkene monooxygenase system, small and larg ...	This protein family includes several components of multicomponent enzyme systems predominantly found in Proteobacteria and Actinobacteria, including subunits alpha and beta of a methane monooxygenase and an alkene monooxygenase system, small and large subunits of propane 2-monooxygenase system and P1 protein of phenol hydroxylase [1-4].	Domain
C, G	PF02964	Methane monooxygenase, hydrolase gamma chain (MeMO_Hyd_G)	Methane monooxygenase, hydrolase gamma chain	-	Domain
D, H	PF02406	MmoB/DmpM family (MmoB_DmpM)	MmoB/DmpM family	This family consists of monooxygenase components such as MmoB methane monooxygenase (EC:1.14.13.25) regulatory protein B. When MmoB is present at low concentration it converts methane monooxygenase from an oxidase to a hydroxylase and stabilises inte ...	This family consists of monooxygenase components such as MmoB methane monooxygenase (EC:1.14.13.25) regulatory protein B. When MmoB is present at low concentration it converts methane monooxygenase from an oxidase to a hydroxylase and stabilises intermediates required for the activation of dioxygen [1]. Also found in this family is DmpM or Phenol hydroxylase (EC:1.14.13.7) protein component P2, this protein lacks redox co-factors and is required for optimal turnover of Phenol hydroxylase [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, E	Methane monooxygenase component A alpha chain	cation binding ion binding binding metal ion binding small molecule binding	cellular metabolic process small molecule metabolic process metabolic process cellular process one-carbon metabolic process	-
B, F	Methane monooxygenase beta chain	oxidoreductase activity monooxygenase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen catalytic activity	-	-
C, G	Methane monooxygenase gamma chain	oxidoreductase activity monooxygenase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen methane monooxygenase [NAD(P)H] activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen catalytic activity	cellular metabolic process cellular alkane metabolic process methane metabolic process metabolic process cellular process hydrocarbon metabolic process	-
D, H	Methane monooxygenase regulatory protein B	monooxygenase activity oxidoreductase activity catalytic activity	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, E	IPR009078	Ferritin-like superfamily	Homologous Superfamily
A, E	IPR003430	Propane/methane/phenol/toluene hydroxylase	Family
A, E	IPR012348	Ribonucleotide reductase-like	Homologous Superfamily
B, F	IPR009078	Ferritin-like superfamily	Homologous Superfamily
B, F	IPR003430	Propane/methane/phenol/toluene hydroxylase	Family
B, F	IPR012078	Methane/phenol monooxygenase, hydroxylase component	Family
B, F	IPR012348	Ribonucleotide reductase-like	Homologous Superfamily
C, G	IPR015952	Methane monooxygenase, gamma chain, domain 1	Homologous Superfamily
C, G	IPR015953	Methane monooxygenase, gamma chain, domain 2	Homologous Superfamily
C, G	IPR036123	Methane monooxygenase, gamma chain superfamily	Homologous Superfamily
C, G	IPR004222	Methane monooxygenase, gamma chain	Family
D, H	IPR036889	Monooxygenase component MmoB/DmpM superfamily	Homologous Superfamily
D, H	IPR003454	Monooxygenase component MmoB/DmpM	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.