7WYX

Cryo-EM structure of Na+,K+-ATPase in the E2P state formed by ATP with istaroxime

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	E1-E2_ATPase_N	e7wyxA4	A: beta sandwiches	X: jelly-roll	H: Calcium ATPase, transduction domain A (From Topology)	T: Calcium ATPase, transduction domain A	F: E1-E2_ATPase_N	ECOD (1.6)
A	Cation_ATPase_C	e7wyxA2	A: alpha bundles	X: Calcium ATPase transmembrane domain-related (From Homology)	H: Calcium ATPase transmembrane domain-related	T: Calcium ATPase transmembrane domain M	F: Cation_ATPase_C	ECOD (1.6)
A	Cation_ATPase	e7wyxA3	A: a+b complex topology	X: Metal cation-transporting ATPase, ATP-binding domain (From Topology)	H: Metal cation-transporting ATPase, ATP-binding domain (From Topology)	T: Metal cation-transporting ATPase, ATP-binding domain	F: Cation_ATPase	ECOD (1.6)
A	Hydrolase_3_1	e7wyxA1	A: a/b three-layered sandwiches	X: HAD domain-like	H: HAD domain-related	T: HAD-like	F: Hydrolase_3_1	ECOD (1.6)
D [auth C]	E1-E2_ATPase_N	e7wyxC3	A: beta sandwiches	X: jelly-roll	H: Calcium ATPase, transduction domain A (From Topology)	T: Calcium ATPase, transduction domain A	F: E1-E2_ATPase_N	ECOD (1.6)
D [auth C]	Cation_ATPase_C	e7wyxC1	A: alpha bundles	X: Calcium ATPase transmembrane domain-related (From Homology)	H: Calcium ATPase transmembrane domain-related	T: Calcium ATPase transmembrane domain M	F: Cation_ATPase_C	ECOD (1.6)
D [auth C]	Cation_ATPase	e7wyxC4	A: a+b complex topology	X: Metal cation-transporting ATPase, ATP-binding domain (From Topology)	H: Metal cation-transporting ATPase, ATP-binding domain (From Topology)	T: Metal cation-transporting ATPase, ATP-binding domain	F: Cation_ATPase	ECOD (1.6)
D [auth C]	Hydrolase_3_1	e7wyxC2	A: a/b three-layered sandwiches	X: HAD domain-like	H: HAD domain-related	T: HAD-like	F: Hydrolase_3_1	ECOD (1.6)
B	Na_K-ATPase_C	e7wyxB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: Na_K-ATPase_C	ECOD (1.6)
B	Na_K-ATPase_N	e7wyxB2	A: extended segments	X: Transmembrane helix in sodium-potassium ATPase beta subunit (From Topology)	H: Transmembrane helix in sodium-potassium ATPase beta subunit (From Topology)	T: Transmembrane helix in sodium-potassium ATPase beta subunit	F: Na_K-ATPase_N	ECOD (1.6)
E [auth D]	Na_K-ATPase_C	e7wyxD1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: Na_K-ATPase_C	ECOD (1.6)
E [auth D]	Na_K-ATPase_N	e7wyxD2	A: extended segments	X: Transmembrane helix in sodium-potassium ATPase beta subunit (From Topology)	H: Transmembrane helix in sodium-potassium ATPase beta subunit (From Topology)	T: Transmembrane helix in sodium-potassium ATPase beta subunit	F: Na_K-ATPase_N	ECOD (1.6)
C [auth G]	ATP1G1_PLM_MAT8	e7wyxG1	A: extended segments	X: FXYD family (From Topology)	H: FXYD family (From Topology)	T: FXYD family	F: ATP1G1_PLM_MAT8	ECOD (1.6)
F [auth E]	ATP1G1_PLM_MAT8	e7wyxE1	A: extended segments	X: FXYD family (From Topology)	H: FXYD family (From Topology)	T: FXYD family	F: ATP1G1_PLM_MAT8	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, D [auth C]	PF00689	Cation transporting ATPase, C-terminus (Cation_ATPase_C)	Cation transporting ATPase, C-terminus	-	Family
A, D [auth C]	PF00702	haloacid dehalogenase-like hydrolase (Hydrolase)	haloacid dehalogenase-like hydrolase	This family is structurally different from the alpha/beta hydrolase family (Pfam:PF00561). This family includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. The structure of the family consists of two domains. One is an inserted fo ...	This family is structurally different from the alpha/beta hydrolase family (Pfam:PF00561). This family includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. The structure of the family consists of two domains. One is an inserted four helix bundle, which is the least well conserved region of the alignment, between residues 16 and 96 of Swiss:P24069. The rest of the fold is composed of the core alpha/beta domain [1]. Those members with the characteristic DxD triad at the N-terminus are probably phosphatidylglycerolphosphate (PGP) phosphatases involved in cardiolipin biosynthesis in the mitochondria [2].	Domain
A, D [auth C]	PF00690	Cation transporter/ATPase, N-terminus (Cation_ATPase_N)	Cation transporter/ATPase, N-terminus	Members of this families are involved in Na+/K+, H+/K+, Ca++ and Mg++ transport.	Domain
A, D [auth C]	PF00122	E1-E2 ATPase (E1-E2_ATPase)	E1-E2 ATPase	-	Family
A, D [auth C]	PF13246	Cation transport ATPase (P-type) (Cation_ATPase)	Cation transport ATPase (P-type)	-	Family
C [auth G], F [auth E]	PF02038	ATP1G1/PLM/MAT8 family (ATP1G1_PLM_MAT8)	ATP1G1/PLM/MAT8 family	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, D [auth C]	Sodium/potassium-transporting ATPase subunit alpha	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity ATP-dependent activity monoatomic cation transmembrane transporter activity P-type transmembrane transporter activity P-type sodium transporter activity potassium ion transmembrane transporter activity primary active transmembrane transporter activity ATPase-coupled transmembrane transporter activity P-type sodium:potassium-exchanging transporter activity ATPase-coupled monoatomic cation transmembrane transporter activity sodium ion transmembrane transporter activity P-type potassium transmembrane transporter activity P-type ion transporter activity active monoatomic ion transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity metal ion transmembrane transporter activity transmembrane transporter activity cation binding metal ion binding	potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis transport potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis transport proton transmembrane transport monoatomic ion transmembrane transport inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization monoatomic ion transport cellular process monoatomic cation transport sodium ion export across plasma membrane export from cell sodium ion transmembrane transport export across plasma membrane metal ion transport sodium ion transport import into cell import across plasma membrane potassium ion transmembrane transport inorganic cation import across plasma membrane inorganic ion import across plasma membrane potassium ion transport potassium ion import across plasma membrane	membrane cellular anatomical entity cell periphery plasma membrane
B, E [auth D]	Na+,K+-ATPase beta subunit	ATPase activator activity molecular function activator activity molecular function regulator activity ATPase regulator activity	potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis transport potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis transport sodium ion export across plasma membrane monoatomic ion transmembrane transport export from cell sodium ion transmembrane transport inorganic ion transmembrane transport export across plasma membrane establishment of localization metal ion transport inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization monoatomic ion transport cellular process monoatomic cation transport sodium ion transport import into cell import across plasma membrane potassium ion transmembrane transport inorganic cation import across plasma membrane inorganic ion import across plasma membrane potassium ion transport potassium ion import across plasma membrane	ATPase dependent transmembrane transport complex protein-containing complex plasma membrane protein complex membrane plasma membrane membrane protein complex sodium:potassium-exchanging ATPase complex cellular anatomical entity transporter complex cation-transporting ATPase complex cell periphery transmembrane transporter complex
C [auth G], F [auth E]	FXYD domain-containing ion transport regulator	channel regulator activity ion channel regulator activity molecular function regulator activity sodium channel regulator activity transporter regulator activity	regulation of localization localization regulation of biological process regulation of transport transport regulation of monoatomic ion transport biological regulation monoatomic ion transport establishment of localization	membrane cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, D [auth C]	IPR005775	P-type ATPase subfamily IIC, subunit alpha	Family
A, D [auth C]	IPR004014	Cation-transporting P-type ATPase, N-terminal	Domain
A, D [auth C]	IPR023214	HAD superfamily	Homologous Superfamily
A, D [auth C]	IPR008250	P-type ATPase, A domain superfamily	Homologous Superfamily
A, D [auth C]	IPR001757	P-type ATPase	Family
A, D [auth C]	IPR044492	P-type ATPase, haloacid dehalogenase domain	Domain
A, D [auth C]	IPR006068	Cation-transporting P-type ATPase, C-terminal	Domain
A, D [auth C]	IPR018303	P-type ATPase, phosphorylation site	PTM
A, D [auth C]	IPR036412	HAD-like superfamily	Homologous Superfamily
A, D [auth C]	IPR023298	P-type ATPase, transmembrane domain superfamily	Homologous Superfamily
A, D [auth C]	IPR023299	P-type ATPase, cytoplasmic domain N	Homologous Superfamily
A, D [auth C]	IPR050510	Cation transport ATPase (P-type)	Family
B, E [auth D]	IPR000402	Sodium/potassium-transporting ATPase subunit beta	Family
B, E [auth D]	IPR038702	Sodium/potassium-transporting ATPase subunit beta superfamily	Homologous Superfamily
C [auth G], F [auth E]	IPR047297	Ion-transport regulator, FXYD motif	Conserved Site
C [auth G], F [auth E]	IPR000272	Ion-transport regulator, FXYD family	Family

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C [auth G], D [auth C], E [auth D] A, B, C [auth G], D [auth C], E [auth D], F [auth E]	OPM	Transmembrane	Alpha-helical polytopic	P-type ATPase (P-ATPase)	P-ATPase

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C [auth G], D [auth C], E [auth D] A, B, C [auth G], D [auth C], E [auth D], F [auth E]	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B, C [auth G], D [auth C], E [auth D] A, B, C [auth G], D [auth C], E [auth D], F [auth E]	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C [auth G], D [auth C], E [auth D] A, B, C [auth G], D [auth C], E [auth D], F [auth E]	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	P-type ATPase	Na,K-ATPase in complex with istaroxime

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A, D [auth C]	PHD	Parent Component: ASP RESID: AA0033 PSI-MOD : L-aspartic 4-phosphoric anhydride MOD:00042

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.