8ED4

Structure of the complex between the arsenite oxidase and its native electron acceptor cytochrome c552 from Pseudorhizobium sp. str. NT-26

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Formate dehydrogenase/DMSO reductase, domains 1-3	8099846	3001453	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	ADC-like	8101088	3001132	SCOP2B (2022-06-29)
D [auth C]	SCOP2B Superfamily	Formate dehydrogenase/DMSO reductase, domains 1-3	8099846	3001453	SCOP2B (2022-06-29)
D [auth C]	SCOP2B Superfamily	ADC-like	8101088	3001132	SCOP2B (2022-06-29)
G [auth E]	SCOP2B Superfamily	ADC-like	8101088	3001132	SCOP2B (2022-06-29)
G [auth E]	SCOP2B Superfamily	Formate dehydrogenase/DMSO reductase, domains 1-3	8099846	3001453	SCOP2B (2022-06-29)
J [auth G]	SCOP2B Superfamily	Formate dehydrogenase/DMSO reductase, domains 1-3	8099846	3001453	SCOP2B (2022-06-29)
J [auth G]	SCOP2B Superfamily	ADC-like	8101088	3001132	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	ISP domain	8099848	3000233	SCOP2B (2022-06-29)
E [auth D]	SCOP2B Superfamily	ISP domain	8099848	3000233	SCOP2B (2022-06-29)
H [auth F]	SCOP2B Superfamily	ISP domain	8099848	3000233	SCOP2B (2022-06-29)
K [auth H]	SCOP2B Superfamily	ISP domain	8099848	3000233	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	F_UNCLASSIFIED	e8ed4A3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: double psi	F: F_UNCLASSIFIED	ECOD (1.6)
A	F_UNCLASSIFIED	e8ed4A4	A: a+b two layers	X: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	H: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domain 1	F: F_UNCLASSIFIED	ECOD (1.6)
A	F_UNCLASSIFIED	e8ed4A2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Formate dehydrogenase/DMSO reductase, domains 2 and 3 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domains 2 and 3	F: F_UNCLASSIFIED	ECOD (1.6)
D [auth C]	F_UNCLASSIFIED	e8ed4C3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: double psi	F: F_UNCLASSIFIED	ECOD (1.6)
D [auth C]	F_UNCLASSIFIED	e8ed4C1	A: a+b two layers	X: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	H: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domain 1	F: F_UNCLASSIFIED	ECOD (1.6)
D [auth C]	F_UNCLASSIFIED	e8ed4C4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Formate dehydrogenase/DMSO reductase, domains 2 and 3 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domains 2 and 3	F: F_UNCLASSIFIED	ECOD (1.6)
G [auth E]	F_UNCLASSIFIED	e8ed4E2	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: double psi	F: F_UNCLASSIFIED	ECOD (1.6)
G [auth E]	F_UNCLASSIFIED	e8ed4E1	A: a+b two layers	X: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	H: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domain 1	F: F_UNCLASSIFIED	ECOD (1.6)
G [auth E]	F_UNCLASSIFIED	e8ed4E4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Formate dehydrogenase/DMSO reductase, domains 2 and 3 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domains 2 and 3	F: F_UNCLASSIFIED	ECOD (1.6)
J [auth G]	F_UNCLASSIFIED	e8ed4G3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: double psi	F: F_UNCLASSIFIED	ECOD (1.6)
J [auth G]	F_UNCLASSIFIED	e8ed4G4	A: a+b two layers	X: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	H: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domain 1	F: F_UNCLASSIFIED	ECOD (1.6)
J [auth G]	F_UNCLASSIFIED	e8ed4G2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Formate dehydrogenase/DMSO reductase, domains 2 and 3 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domains 2 and 3	F: F_UNCLASSIFIED	ECOD (1.6)
B	F_UNCLASSIFIED	e8ed4B1	A: beta sandwiches	X: ISP domain (From Topology)	H: ISP domain (From Topology)	T: ISP domain	F: F_UNCLASSIFIED	ECOD (1.6)
B	F_UNCLASSIFIED	e8ed4B2	A: few secondary structure elements	X: Trm112p-like (From Topology)	H: Trm112p-like (From Topology)	T: Trm112p-like	F: F_UNCLASSIFIED	ECOD (1.6)
E [auth D]	F_UNCLASSIFIED	e8ed4D2	A: beta sandwiches	X: ISP domain (From Topology)	H: ISP domain (From Topology)	T: ISP domain	F: F_UNCLASSIFIED	ECOD (1.6)
E [auth D]	F_UNCLASSIFIED	e8ed4D1	A: few secondary structure elements	X: Trm112p-like (From Topology)	H: Trm112p-like (From Topology)	T: Trm112p-like	F: F_UNCLASSIFIED	ECOD (1.6)
H [auth F]	F_UNCLASSIFIED	e8ed4F1	A: beta sandwiches	X: ISP domain (From Topology)	H: ISP domain (From Topology)	T: ISP domain	F: F_UNCLASSIFIED	ECOD (1.6)
H [auth F]	F_UNCLASSIFIED	e8ed4F2	A: few secondary structure elements	X: Trm112p-like (From Topology)	H: Trm112p-like (From Topology)	T: Trm112p-like	F: F_UNCLASSIFIED	ECOD (1.6)
K [auth H]	F_UNCLASSIFIED	e8ed4H1	A: beta sandwiches	X: ISP domain (From Topology)	H: ISP domain (From Topology)	T: ISP domain	F: F_UNCLASSIFIED	ECOD (1.6)
K [auth H]	F_UNCLASSIFIED	e8ed4H2	A: few secondary structure elements	X: Trm112p-like (From Topology)	H: Trm112p-like (From Topology)	T: Trm112p-like	F: F_UNCLASSIFIED	ECOD (1.6)
C [auth I]	F_UNCLASSIFIED	e8ed4I1	A: alpha arrays	X: Cytochrome c-like	H: Cytochrome c (From Topology)	T: Cytochrome c	F: F_UNCLASSIFIED	ECOD (1.6)
F [auth J]	F_UNCLASSIFIED	e8ed4J1	A: alpha arrays	X: Cytochrome c-like	H: Cytochrome c (From Topology)	T: Cytochrome c	F: F_UNCLASSIFIED	ECOD (1.6)
I [auth K]	F_UNCLASSIFIED	e8ed4K1	A: alpha arrays	X: Cytochrome c-like	H: Cytochrome c (From Topology)	T: Cytochrome c	F: F_UNCLASSIFIED	ECOD (1.6)
L	F_UNCLASSIFIED	e8ed4L1	A: alpha arrays	X: Cytochrome c-like	H: Cytochrome c (From Topology)	T: Cytochrome c	F: F_UNCLASSIFIED	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, D [auth C], G [auth E], J [auth G]	PF01568	Molydopterin dinucleotide binding domain (Molydop_binding)	Molydopterin dinucleotide binding domain	This domain is found in various molybdopterin - containing oxidoreductases and tungsten formylmethanofuran dehydrogenase subunit d (FwdD) and molybdenum formylmethanofuran dehydrogenase subunit (FmdD); where the domain constitutes almost the entir ...	This domain is found in various molybdopterin - containing oxidoreductases and tungsten formylmethanofuran dehydrogenase subunit d (FwdD) and molybdenum formylmethanofuran dehydrogenase subunit (FmdD); where the domain constitutes almost the entire subunit. The formylmethanofuran dehydrogenase catalyses the first step in methane formation from CO2 in methanogenic archaea and has a molybdopterin dinucleotide cofactor [1]. This domain corresponds to the C-terminal domain IV in dimethyl sulfoxide (DMSO)reductase which interacts with the 2-amino pyrimidone ring of both molybdopterin guanine dinucleotide molecules [2].	Domain
A, D [auth C], G [auth E], J [auth G]	PF18465	Rieske 3Fe-4S (Rieske_3)	Rieske 3Fe-4S	This domain is comprised of the iron-sulphur cluster and Rieske subunit found in the large subunit of arsenite oxidase. Arsenite oxidase is a 100 kDa molybdenum- and iron-sulfur-containing protein located on the outer surface of the inner membrane of ...	This domain is comprised of the iron-sulphur cluster and Rieske subunit found in the large subunit of arsenite oxidase. Arsenite oxidase is a 100 kDa molybdenum- and iron-sulfur-containing protein located on the outer surface of the inner membrane of Gram-negative organisms. The large subunit of arsenite oxidase is similar to other members of the dimethylsulfoxide (DMSO) reductase family of molybdenum enzymes. The large subunit of arsenite oxidase is divided into four domains, with domain I binding the [3Fe-4S] cluster . Domain I, consists of three antiparallel beta sheets and six helices. The [3Fe-4S] cluster is coordinated by the motif Cys21-X2-Cys24-X3-Cys28 near the interface with domains III and IV. A large, flattened funnel-like cavity bounded by domains I, II, and III leads to the molybdenum center Pfam:PF00384 located near the center of the molecule [1].	Domain
A, D [auth C], G [auth E], J [auth G]	PF00384	Molybdopterin oxidoreductase (Molybdopterin)	Molybdopterin oxidoreductase	-	Family
B, E [auth D], H [auth F], K [auth H]	PF00355	Rieske [2Fe-2S] domain (Rieske)	Rieske [2Fe-2S] domain	The rieske domain has a [2Fe-2S] centre. Two conserved cysteines coordinate one Fe ion, while the other Fe ion is coordinated by two conserved histidines. In hyperthermophilic archaea there is a SKTPCX(2-3)C motif at the C-terminus. The cysteines in ...	The rieske domain has a [2Fe-2S] centre. Two conserved cysteines coordinate one Fe ion, while the other Fe ion is coordinated by two conserved histidines. In hyperthermophilic archaea there is a SKTPCX(2-3)C motif at the C-terminus. The cysteines in this motif form a disulphide bridge, which stabilises the protein [4].	Domain
C [auth I], F [auth J], I [auth K], L	PF00034	Cytochrome c (Cytochrom_C)	Cytochrome c	The Pfam entry does not include all Prosite members. The cytochrome 556 and cytochrome c' families are not included. All these are now in a new clan together. The C-terminus of DUF989, Pfam:PF06181, has now been merged into this family.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, D [auth C], G [auth E], J [auth G]	AroA	oxidoreductase activity catalytic activity iron-sulfur cluster binding binding 3 iron, 4 sulfur cluster binding metal cluster binding small molecule binding organic cyclic compound binding molybdopterin cofactor binding cation binding ion binding metal ion binding	cellular respiration energy derivation by oxidation of organic compounds cellular metabolic process metabolic process cellular process generation of precursor metabolites and energy	protein-containing complex catalytic complex oxidoreductase complex
B, E [auth D], H [auth F], K [auth H]	AroB	iron-sulfur cluster binding 2 iron, 2 sulfur cluster binding binding metal cluster binding small molecule binding cation binding ion binding metal ion binding	-	-
C [auth I], F [auth J], I [auth K], L	C-type cytochrome c552	binding organic cyclic compound binding tetrapyrrole binding heme binding electron transfer activity cation binding ion binding metal ion binding small molecule binding	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, D [auth C], G [auth E], J [auth G]	IPR006657	Molybdopterin dinucleotide-binding domain	Domain
A, D [auth C], G [auth E], J [auth G]	IPR006656	Molybdopterin oxidoreductase	Domain
A, D [auth C], G [auth E], J [auth G]	IPR050123	Prokaryotic molybdopterin-containing oxidoreductase	Family
A, D [auth C], G [auth E], J [auth G]	IPR014066	Arsenite oxidase subunit AioA/Iodate reductase subunit IdrA, large subunit	Family
A, D [auth C], G [auth E], J [auth G]	IPR041632	Arsenite oxidase subunit AioA/Iodate reductase subunit IdrA, 3Fe-4S cluster	Domain
A, D [auth C], G [auth E], J [auth G]	IPR009010	Aspartate decarboxylase-like domain superfamily	Homologous Superfamily
B, E [auth D], H [auth F], K [auth H]	IPR017941	Rieske [2Fe-2S] iron-sulphur domain	Domain
B, E [auth D], H [auth F], K [auth H]	IPR036922	Rieske [2Fe-2S] iron-sulphur domain superfamily	Homologous Superfamily
B, E [auth D], H [auth F], K [auth H]	IPR006311	Twin-arginine translocation pathway, signal sequence	Conserved Site
B, E [auth D], H [auth F], K [auth H]	IPR014067	Arsenite oxidase subunit AioB/Iodate reductase subunit IdrB, small subunit	Family
C [auth I], F [auth J], I [auth K], L	IPR036909	Cytochrome c-like domain superfamily	Homologous Superfamily
C [auth I], F [auth J], I [auth K], L	IPR009056	Cytochrome c-like domain	Domain
C [auth I], F [auth J], I [auth K], L	IPR002327	Cytochrome c, class IA/ IB	Family