NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.8 mM [U-99% 13C; U-99% 15N] de novo protein RO2_20, 21.1 mM potassium phosphate monobasic, 28.9 mM sodium phosphate dibasic | 90% H2O/10% D2O | 0 M | 7.0 | 1 atm | 300 | Bruker AVANCE 800 |
2 | 2D 1H-13C HSQC | 0.8 mM [U-99% 13C; U-99% 15N] de novo protein RO2_20, 21.1 mM potassium phosphate monobasic, 28.9 mM sodium phosphate dibasic | 90% H2O/10% D2O | 0 M | 7.0 | 1 atm | 300 | Bruker AVANCE 800 |
3 | 3D CBCA(CO)NH | 0.8 mM [U-99% 13C; U-99% 15N] de novo protein RO2_20, 21.1 mM potassium phosphate monobasic, 28.9 mM sodium phosphate dibasic | 90% H2O/10% D2O | 0 M | 7.0 | 1 atm | 300 | Bruker DRX 500 |
4 | 3D HNCACB | 0.8 mM [U-99% 13C; U-99% 15N] de novo protein RO2_20, 21.1 mM potassium phosphate monobasic, 28.9 mM sodium phosphate dibasic | 90% H2O/10% D2O | 0 M | 7.0 | 1 atm | 300 | Bruker DRX 500 |
5 | 3D HCCH-TOCSY | 0.8 mM [U-99% 13C; U-99% 15N] de novo protein RO2_20, 21.1 mM potassium phosphate monobasic, 28.9 mM sodium phosphate dibasic | 90% H2O/10% D2O | 0 M | 7.0 | 1 atm | 300 | Bruker AVANCE 800 |
6 | 3D 1H-15N NOESY | 0.8 mM [U-99% 13C; U-99% 15N] de novo protein RO2_20, 21.1 mM potassium phosphate monobasic, 28.9 mM sodium phosphate dibasic | 90% H2O/10% D2O | 0 M | 7.0 | 1 atm | 300 | Bruker AVANCE 800 |
7 | 3D 1H-13C NOESY | 0.8 mM [U-99% 13C; U-99% 15N] de novo protein RO2_20, 21.1 mM potassium phosphate monobasic, 28.9 mM sodium phosphate dibasic | 90% H2O/10% D2O | 0 M | 7.0 | 1 atm | 300 | Bruker AVANCE 800 |