Download MendeleyStaphylococcus aureus 3-hydroxy-3-methylglutaryl-CoA synthase: crystal structure and mechanism
Campobasso, N., Patel, M., Wilding, I.E., Kallender, H., Rosenberg, M., Gwynn, M.(2004) J Biol Chem 279: 44883-44888
- PubMed: 15292254
- DOI: https://doi.org/10.1074/jbc.M407882200
- Primary Citation of Related Structures:
1TVZ, 1TXT - PubMed Abstract:
3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, a member of the family of acyl-condensing enzymes, catalyzes the first committed step in the mevalonate pathway and is a potential target for novel antibiotics and cholesterol-lowering agents. The Staphylococcus aureus mvaS gene product (43.2 kDa) was overexpressed in Escherichia coli, purified to homogeneity, and shown biochemically to be an HMG-CoA synthase. The crystal structure of the full-length enzyme was determined at 2.0-A resolution, representing the first structure of an HMG-CoA synthase from any organism. HMG-CoA synthase forms a homodimer. The monomer, however, contains an important core structure of two similar alpha/beta motifs, a fold that is completely conserved among acyl-condensing enzymes. This common fold provides a scaffold for a catalytic triad made up of Cys, His, and Asn required by these enzymes. In addition, a crystal structure of HMG-CoA synthase with acetoacetyl-CoA was determined at 2.5-A resolution. Together, these structures provide the structural basis for an understanding of the mechanism of HMG-CoA synthase.
Organizational Affiliation:
GlaxoSmithKline Pharmaceuticals, Computational, Analytical, and Structural Sciences, King of Prussia, Pennsylvania 19406, USA. [email protected]
