3L9I

Myosin VI nucleotide-free (mdinsert2) L310G mutant crystal structure

PDB DOI: https://doi.org/10.2210/pdb3L9I/pdb

Classification: MOTOR PROTEIN
Organism(s): Sus scrofa, Drosophila melanogaster
Expression System: Spodoptera frugiperda
Mutation(s): Yes

Deposited: 2010-01-05 Released: 2010-12-08
Deposition Author(s): Pylypenko, O., Song, L., Sweeney, L.H., Houdusse, A.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.20 Å
R-Value Free: 0.235
R-Value Work: 0.185
R-Value Observed: 0.187

Starting Model: experimental
View more details

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Role of insert i of myosin VI in modulating nucleotide affinity

Pylypenko, O., Song, L., Squires, G., Liu, X., Zong, A.B., Houdusse, A., Sweeney, L.H.

To be published.

Macromolecule Content

Total Structure Weight: 110.53 kDa
Atom Count: 8,566
Modelled Residue Count: 936
Deposited Residue Count: 963
Unique protein chains: 2

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Myosin-VI	A	814	Sus scrofa	Mutation(s): 1 Gene Names: MYO6
UniProt
Find proteins for Q29122 (Sus scrofa) Explore Q29122 Go to UniProtKB: Q29122
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q29122
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
Calmodulin	B [auth C]	149	Drosophila melanogaster	Mutation(s): 0 Gene Names: Cam, CG8472
UniProt
Find proteins for P62152 (Drosophila melanogaster) Explore P62152 Go to UniProtKB: P62152
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P62152
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 4 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
PO4 Query on PO4 Download Ideal Coordinates CCD File SDF format, chain F [auth A] SDF format, chain G [auth A] SDF format, chain H [auth A] SDF format, chain E [auth A] MOL2 format, chain F [auth A] MOL2 format, chain G [auth A] MOL2 format, chain H [auth A] MOL2 format, chain E [auth A] mmCIF format, chain F [auth A] mmCIF format, chain G [auth A] mmCIF format, chain H [auth A] mmCIF format, chain E [auth A]	E [auth A], F [auth A], G [auth A], H [auth A]	PHOSPHATE ION O₄ P NBIIXXVUZAFLBC-UHFFFAOYSA-K		Interactions Focus chain E [auth A] Focus chain F [auth A] Focus chain G [auth A] Focus chain H [auth A] Interactions & Density Focus chain E [auth A] Focus chain F [auth A] Focus chain G [auth A] Focus chain H [auth A]
TBU Query on TBU Download Ideal Coordinates CCD File SDF format, chain C [auth A] MOL2 format, chain C [auth A] mmCIF format, chain C [auth A]	C [auth A]	TERTIARY-BUTYL ALCOHOL C₄ H₁₀ O DKGAVHZHDRPRBM-UHFFFAOYSA-N		Interactions Focus chain C [auth A] Interactions & Density Focus chain C [auth A]
ACT Query on ACT Download Ideal Coordinates CCD File SDF format, chain D [auth A] SDF format, chain I [auth A] MOL2 format, chain D [auth A] MOL2 format, chain I [auth A] mmCIF format, chain D [auth A] mmCIF format, chain I [auth A]	D [auth A], I [auth A]	ACETATE ION C₂ H₃ O₂ QTBSBXVTEAMEQO-UHFFFAOYSA-M		Interactions Focus chain D [auth A] Focus chain I [auth A] Interactions & Density Focus chain D [auth A] Focus chain I [auth A]
CA Query on CA Download Ideal Coordinates CCD File SDF format, chain J [auth C] SDF format, chain K [auth C] SDF format, chain M [auth C] SDF format, chain L [auth C] MOL2 format, chain J [auth C] MOL2 format, chain K [auth C] MOL2 format, chain M [auth C] MOL2 format, chain L [auth C] mmCIF format, chain J [auth C] mmCIF format, chain K [auth C] mmCIF format, chain M [auth C] mmCIF format, chain L [auth C]	J [auth C], K [auth C], L [auth C], M [auth C]	CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N		Interactions Focus chain J [auth C] Focus chain K [auth C] Focus chain L [auth C] Focus chain M [auth C] Interactions & Density Focus chain J [auth C] Focus chain K [auth C] Focus chain L [auth C] Focus chain M [auth C]