Download MendeleyCrystal Structure of the C194A mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ1
Kim, Y., Zhou, M., Gu, M., Anderson, W.F., Joachimiak, A., CSGIDTo be published.
3RZP
Crystal Structure of the C194A mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ1
- PDB DOI: https://doi.org/10.2210/pdb3RZP/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Vibrio cholerae
- Expression System: Escherichia coli BL21
- Mutation(s): Yes
- Deposited: 2011-05-12 Released: 2011-06-29
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.221
- R-Value Work: 0.171
- R-Value Observed: 0.174
Starting Model: experimental
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This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| NADPH-dependent 7-cyano-7-deazaguanine reductase | 290 | Vibrio cholerae | Mutation(s): 1 Gene Names: queF, VC_0902 EC: 1.7.1.13 |  | |
UniProt | |||||
Find proteins for Q9KTK0 (Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)) Explore Q9KTK0 Go to UniProtKB: Q9KTK0 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9KTK0 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PRF Query on PRF | E [auth A], G [auth B], H [auth C], I [auth D] | 7-DEAZA-7-AMINOMETHYL-GUANINE C7 H9 N5 O MEYMBLGOKYDGLZ-UHFFFAOYSA-N |  | ||
| GOL Query on GOL | F [auth A], J [auth D] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, B, C, D | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.221
- R-Value Work: 0.171
- R-Value Observed: 0.174
- Space Group: P 1
- Diffraction Data: https://doi.org/10.18430/m33rzp
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 71.316 | α = 110.43 |
| b = 71.192 | β = 119.22 |
| c = 71.463 | γ = 99.33 |
| Software Name | Purpose |
|---|---|
| SBC-Collect | data collection |
| HKL-3000 | data collection |
| HKL-3000 | phasing |
| MOLREP | phasing |
| PHENIX | refinement |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
Entry History
Deposition Data
- Released Date: 2011-06-29 Deposition Author(s): Kim, Y., Zhou, M., Gu, M., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)
Revision History (Full details and data files)
- Version 1.0: 2011-06-29
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2023-09-13
Changes: Data collection, Database references, Derived calculations, Refinement description - Version 1.3: 2023-12-06
Changes: Data collection
