Download MendeleyAn Anti-Tubulin Darpin Caps the Microtubule Plus-End
Pecqueur, L., Duellberg, C., Dreier, B., Wang, Q., Jiang, C., Pluckthun, A., Surrey, T., Gigant, B., Knossow, M.To be published.
4DUI
DARPIN D1 binding to tubulin beta chain (not in complex)
- PDB DOI: https://doi.org/10.2210/pdb4DUI/pdb
- Classification: DE NOVO PROTEIN
- Organism(s): synthetic construct
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2012-02-22 Released: 2013-02-27
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.16 Å
- R-Value Free: 0.149
- R-Value Work: 0.123
- R-Value Observed: 0.125
Starting Model: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DESIGNED ANKYRIN REPEAT PROTEIN (DARPIN) D1 | 169 | synthetic construct | Mutation(s): 0 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MRD Query on MRD | C [auth A], D [auth A] | (4R)-2-METHYLPENTANE-2,4-DIOL C6 H14 O2 SVTBMSDMJJWYQN-RXMQYKEDSA-N |  | ||
| MPD Query on MPD | B [auth A], E [auth A] | (4S)-2-METHYL-2,4-PENTANEDIOL C6 H14 O2 SVTBMSDMJJWYQN-YFKPBYRVSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| SME Query on SME | A | L-PEPTIDE LINKING | C5 H11 N O3 S |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.16 Å
- R-Value Free: 0.149
- R-Value Work: 0.123
- R-Value Observed: 0.125
- Space Group: P 41 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 43.4 | α = 90 |
| b = 43.4 | β = 90 |
| c = 143.12 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHASER | phasing |
| PHENIX | refinement |
| XDS | data reduction |
| XSCALE | data scaling |
Entry History
Deposition Data
- Released Date: 2013-02-27 Deposition Author(s): Pecqueur, L., Duellberg, C., Dreier, B., Wang, Q., Jiang, C., Pluckthun, A., Surrey, T., Gigant, B., Knossow, M.
Revision History (Full details and data files)
- Version 1.0: 2013-02-27
Type: Initial release - Version 1.1: 2023-09-13
Changes: Data collection, Database references, Derived calculations, Refinement description
