Download MendeleyCatalytic Mechanism and Allosteric Regulation of Udp-Glucose Pyrophosphorylase from Leishmania Major
Fuehring, J., Routier, F.H., Lamerz, A.-C., Baruch, P., Gerardy-Schahn, R., Fedorov, R.(2013) ACS Catal 36329: 2976-2985
4M2B
Crystal structure of L281D mutant of udp-glucose pyrophosphorylase from leishmania major in complex with udp-glc
- PDB DOI: https://doi.org/10.2210/pdb4M2B/pdb
- Classification: TRANSFERASE
- Organism(s): Leishmania major
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2013-08-05 Released: 2014-01-29
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.242
- R-Value Work: 0.175
- R-Value Observed: 0.178
Starting Model: experimental
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This is version 1.3 of the entry. See complete history.
Literature
Macromolecules
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(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| UDP-glucose pyrophosphorylase | 505 | Leishmania major | Mutation(s): 1 Gene Names: LMJF_18_0990, UGP EC: 2.7.7.9 |  | |
UniProt | |||||
Find proteins for Q4QDU3 (Leishmania major) Explore Q4QDU3 Go to UniProtKB: Q4QDU3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q4QDU3 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| UPG Query on UPG | B [auth A] | URIDINE-5'-DIPHOSPHATE-GLUCOSE C15 H24 N2 O17 P2 HSCJRCZFDFQWRP-JZMIEXBBSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.242
- R-Value Work: 0.175
- R-Value Observed: 0.178
- Space Group: C 2 2 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 78.79 | α = 90 |
| b = 86.9 | β = 90 |
| c = 138.23 | γ = 90 |
| Software Name | Purpose |
|---|---|
| MxCuBE | data collection |
| CCP4 | model building |
| REFMAC | refinement |
| XDS | data reduction |
| SADABS | data scaling |
| CCP4 | phasing |
Entry History
Deposition Data
- Released Date: 2014-01-29 Deposition Author(s): Fuehring, J., Routier, F.H., Lamerz, A.-C., Baruch, P., Gerardy-Schahn, R., Fedorov, R.
Revision History (Full details and data files)
- Version 1.0: 2014-01-29
Type: Initial release - Version 1.1: 2014-02-19
Changes: Database references - Version 1.2: 2023-09-20
Changes: Data collection, Database references, Derived calculations, Refinement description - Version 1.3: 2024-10-16
Changes: Structure summary
