Download MendeleyCrystal structure of NAD kinase 1 from Listeria monocytogenes: V98S mutant
Poncet-Montange, G., Assairi, L., Gelin, M., Pochet, S., Labesse, G.To be published.
5EJH
Crystal structure of NAD kinase V98S mutant from Listeria monocytogenes
- PDB DOI: https://doi.org/10.2210/pdb5EJH/pdb
- Classification: TRANSFERASE
- Organism(s): Listeria monocytogenes
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2015-11-01 Released: 2016-11-09
- Funding Organization(s): French National Research Agency
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.221
- R-Value Work: 0.177
- R-Value Observed: 0.178
Starting Model: experimental
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This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| NAD kinase 1 | 272 | Listeria monocytogenes | Mutation(s): 1 Gene Names: nadK1, lmo0968 EC: 2.7.1.23 |  | |
UniProt | |||||
Find proteins for Q8Y8D7 (Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)) Explore Q8Y8D7 Go to UniProtKB: Q8Y8D7 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q8Y8D7 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| NAP Query on NAP | B [auth A] | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE C21 H28 N7 O17 P3 XJLXINKUBYWONI-NNYOXOHSSA-N |  | ||
| CIT Query on CIT | C [auth A] | CITRIC ACID C6 H8 O7 KRKNYBCHXYNGOX-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.221
- R-Value Work: 0.177
- R-Value Observed: 0.178
- Space Group: I 2 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 62.759 | α = 90 |
| b = 75.88 | β = 90 |
| c = 118.207 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| SCALA | data scaling |
| PDB_EXTRACT | data extraction |
| MOSFLM | data reduction |
Entry History & Funding Information
Deposition Data
- Released Date: 2016-11-09 Deposition Author(s): Poncet-Montange, G., Assairi, L., Gelin, M., Pochet, S., Labesse, G.
| Funding Organization | Location | Grant Number |
|---|---|---|
| French National Research Agency | France | -- |
Revision History (Full details and data files)
- Version 1.0: 2016-11-09
Type: Initial release - Version 1.1: 2017-09-06
Changes: Author supporting evidence, Data collection - Version 1.2: 2024-01-10
Changes: Data collection, Database references, Refinement description
