5JK5

Phenylalanine hydroxylase from dictyostelium - BH2 complex

PDB DOI: https://doi.org/10.2210/pdb5JK5/pdb

Classification: OXIDOREDUCTASE
Organism(s): Dictyostelium discoideum
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2016-04-26 Released: 2017-04-26
Deposition Author(s): Zhuang, N., Lee, K.H.
Funding Organization(s): National research foundation

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.07 Å
R-Value Free: 0.228
R-Value Work: 0.165
R-Value Observed: 0.168

wwPDB Validation 3D Report Full Report

Ligand Structure Quality Assessment

This is version 1.1 of the entry. See complete history.

Literature

Phenylalanine hydroxylase from dictyostelium - BH2 complex

Zhuang, N., Lee, K.H.

To be published.

Macromolecule Content

Total Structure Weight: 101.29 kDa
Atom Count: 6,862
Modelled Residue Count: 791
Deposited Residue Count: 876
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Phenylalanine-4-hydroxylase	A, B	438	Dictyostelium discoideum	Mutation(s): 0 Gene Names: pah, DDB_G0278781 EC: 1.14.16.1 (PDB Primary Data), 1.14.16.4 (PDB Primary Data)
UniProt
Find proteins for Q54XS1 (Dictyostelium discoideum) Explore Q54XS1 Go to UniProtKB: Q54XS1
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q54XS1
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 3 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
PIN Query on PIN Download Ideal Coordinates CCD File SDF format, chain D [auth A] SDF format, chain G [auth B] MOL2 format, chain D [auth A] MOL2 format, chain G [auth B] mmCIF format, chain D [auth A] mmCIF format, chain G [auth B]	D [auth A], G [auth B]	PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) C₈ H₁₈ N₂ O₆ S₂ IHPYMWDTONKSCO-UHFFFAOYSA-N		Interactions Focus chain D [auth A] Focus chain G [auth B] Interactions & Density Focus chain D [auth A] Focus chain G [auth B]
HBI Query on HBI Download Ideal Coordinates CCD File SDF format, chain E [auth A] SDF format, chain H [auth B] MOL2 format, chain E [auth A] MOL2 format, chain H [auth B] mmCIF format, chain E [auth A] mmCIF format, chain H [auth B]	E [auth A], H [auth B]	7,8-DIHYDROBIOPTERIN C₉ H₁₃ N₅ O₃ FEMXZDUTFRTWPE-DZSWIPIPSA-N		Interactions Focus chain E [auth A] Focus chain H [auth B] Interactions & Density Focus chain E [auth A] Focus chain H [auth B]
FE Query on FE Download Ideal Coordinates CCD File SDF format, chain C [auth A] SDF format, chain F [auth B] MOL2 format, chain C [auth A] MOL2 format, chain F [auth B] mmCIF format, chain C [auth A] mmCIF format, chain F [auth B]	C [auth A], F [auth B]	FE (III) ION Fe VTLYFUHAOXGGBS-UHFFFAOYSA-N		Interactions Focus chain C [auth A] Focus chain F [auth B] Interactions & Density Focus chain C [auth A] Focus chain F [auth B]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.07 Å
R-Value Free: 0.228
R-Value Work: 0.165
R-Value Observed: 0.168
Space Group: P 1 2₁ 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 70.968	α = 90
b = 85.326	β = 110.23
c = 74.894	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
HKL-2000	data reduction
HKL-2000	data scaling
AMoRE	phasing
Coot	model building

Structure Validation

View Full Validation Report

Ligand Structure Quality Assessment

Entry History & Funding Information

Deposition Data

Released Date: 2017-04-26

Deposition Author(s):

Zhuang, N.

Lee, K.H.

Funding Organization	Location	Grant Number
National research foundation	Korea, Republic Of	--

Revision History (Full details and data files)

Version 1.0: 2017-04-26
Type: Initial release
Version 1.1: 2024-11-20
Changes: Data collection, Database references, Derived calculations, Structure summary

Prepare Data

Validate Data

Deposit Data

Help and Resources

5JK5

Phenylalanine hydroxylase from dictyostelium - BH2 complex

Phenylalanine hydroxylase from dictyostelium - BH2 complex

Entity ID: 1

UniProt

Entity Groups

Sequence Annotations

Expand

Experimental Data

Structure Validation

Ligand Structure Quality Assessment

Deposition Data

Revision History (Full details and data files)