Download MendeleyFlexibility of a Distal Interface Loop Modulates Water Network in the Active Site of Bacillus sp. TB-90 Urate Oxidase
Hibi, T., Itoh, T., Nishiya, Y.To be published.
5Z27
Crystal structure of highly active BTUO mutant P287G without dehydration
- PDB DOI: https://doi.org/10.2210/pdb5Z27/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Bacillus sp. TB-90
- Expression System: Escherichia coli DH5[alpha]
- Mutation(s): Yes
- Deposited: 2017-12-29 Released: 2019-01-02
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.60 Å
- R-Value Free: 0.179
- R-Value Work: 0.163
- R-Value Observed: 0.163
Starting Model: experimental
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This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Uric acid degradation bifunctional protein | 323 | Bacillus sp. TB-90 | Mutation(s): 1 Gene Names: uao EC: 1.7.3.3 (PDB Primary Data), 4.1.1.97 (UniProt) |  | |
UniProt | |||||
Find proteins for Q45697 (Bacillus sp. (strain TB-90)) Explore Q45697 Go to UniProtKB: Q45697 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q45697 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 5 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| AZA Query on AZA | C [auth A], G [auth B] | 8-AZAXANTHINE C4 H3 N5 O2 KVGVQTOQSNJTJI-UHFFFAOYSA-N |  | ||
| SO4 Query on SO4 | D [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| EDO Query on EDO | I [auth B], J [auth B] | 1,2-ETHANEDIOL C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N |  | ||
| K Query on K | H [auth B] | POTASSIUM ION K NPYPAHLBTDXSSS-UHFFFAOYSA-N |  | ||
| OXY Query on OXY | E [auth A], F [auth B] | OXYGEN MOLECULE O2 MYMOFIZGZYHOMD-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| OCS Query on OCS | A, B | L-PEPTIDE LINKING | C3 H7 N O5 S |  | CYS |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.60 Å
- R-Value Free: 0.179
- R-Value Work: 0.163
- R-Value Observed: 0.163
- Space Group: I 2 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 70.496 | α = 90 |
| b = 133.047 | β = 90 |
| c = 144.717 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
| PHASER | phasing |
Entry History
Deposition Data
- Released Date: 2019-01-02 Deposition Author(s): Hibi, T., Itoh, T., Nishiya, Y.
Revision History (Full details and data files)
- Version 1.0: 2019-01-02
Type: Initial release - Version 1.1: 2023-11-22
Changes: Data collection, Database references, Derived calculations, Refinement description
