6BIA

The Molecular Basis of Polysaccharide Sulfatase Activity and a Nomenclature for Catalytic Subsites in this Class of Enzyme.

(2018) Structure 26: 747

• PubMed: 29681469 Search on PubMed
• DOI: https://doi.org/10.1016/j.str.2018.03.012
• Primary Citation of Related Structures:  
  6B0J, 6B0K, 6B1V, 6BIA


• PubMed Abstract: 
  

  Sulfatases play a biologically important role by cleaving sulfate groups from molecules. They can be identified on the basis of signature sequences within their primary structures, and the largest family, S1, has predictable features that contribute specifically to the recognition and catalytic removal of sulfate groups. However, despite advances in the prediction and understanding of S1 sulfatases, a major question regards the molecular determinants that drive substrate recognition beyond the targeted sulfate group. Here, through analysis of an endo-4S-ι-carrageenan sulfatase (PsS1_19A) from Pseudoalteromonas sp. PS47, particularly X-ray crystal structures in complex with intact substrates, we show that specific recognition of the substrate leaving group components, in this case carbohydrate, provides the enzyme with specificity for its substrate. On the basis of these results we propose a catalytic subsite nomenclature that we anticipate will form a general foundation for understanding and describing the molecular basis of substrate recognition by sulfatases.

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Organizational Affiliation: 

Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 3P6, Canada.