Download MendeleyThe crystal structure of light-driven cyanobacterial chloride importer (N63A/P118A) Mastigocladopsis repens
Yun, J.H., Park, J.H., Jin, Z., Ohki, M., Wang, Y., Lupala, C.S., Kim, M., Liu, H., Park, S.Y., Lee, W.To be published.
6K6K
The crystal structure of light-driven cyanobacterial chloride importer (N63A/P118A) Mastigocladopsis repens
- PDB DOI: https://doi.org/10.2210/pdb6K6K/pdb
- Classification: MEMBRANE PROTEIN
- Organism(s): Mastigocladopsis repens
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2019-06-03 Released: 2020-06-03
- Funding Organization(s): National Research Foundation (Korea)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.268
- R-Value Work: 0.212
- R-Value Observed: 0.214
Starting Model: experimental
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This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Cyanobacterial chloride importer | 232 | Mastigocladopsis repens | Mutation(s): 0 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| RET (Subject of Investigation/LOI) Query on RET | D [auth A] | RETINAL C20 H28 O NCYCYZXNIZJOKI-OVSJKPMPSA-N |  | ||
| OLA Query on OLA | E [auth A], F [auth A], G [auth A], H [auth A] | OLEIC ACID C18 H34 O2 ZQPPMHVWECSIRJ-KTKRTIGZSA-N |  | ||
| CL Query on CL | B [auth A], C [auth A] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.268
- R-Value Work: 0.212
- R-Value Observed: 0.214
- Space Group: P 63 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 60.972 | α = 90 |
| b = 60.972 | β = 90 |
| c = 205.574 | γ = 120 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| Coot | model building |
| PHENIX | phasing |
| MxDC | data collection |
| HKL-2000 | data scaling |
Entry History & Funding Information
Deposition Data
- Released Date: 2020-06-03 Deposition Author(s): Yun, J.H., Park, J.H., Jin, Z., Ohki, M., Wang, Y., Lupala, C.S., Kim, M., Liu, H., Park, S.Y., Lee, W.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Research Foundation (Korea) | Korea, Republic Of | 20171A2B2008483 |
| National Research Foundation (Korea) | Korea, Republic Of | 20161A6A3A04010213 |
Revision History (Full details and data files)
- Version 1.0: 2020-06-03
Type: Initial release - Version 1.1: 2023-11-22
Changes: Data collection, Database references, Refinement description - Version 1.2: 2024-10-30
Changes: Structure summary
