Download MendeleyFUNCTIONAL CONSEQUENCES OF ONCOGENIC MUTATIONS IN THE SWITCH II REGION OF Galphai1 and Galphas PROTEINS
Goossens, J.L., Leverson, B.D., Kothawala, S., Mascarenhas, R., Liu, D., Ballicora, M., Olsen, K.W., de Freitas, D.M.To be published.
6M8H
Crystal Structure of the R208Q mutant of G(i) subunit alpha-1
- PDB DOI: https://doi.org/10.2210/pdb6M8H/pdb
- Classification: SIGNALING PROTEIN
- Organism(s): Rattus norvegicus
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2018-08-21 Released: 2019-08-21
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.07 Å
- R-Value Free: 0.197
- R-Value Work: 0.165
- R-Value Observed: 0.168
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Guanine nucleotide-binding protein G(i) subunit alpha-1 | 354 | Rattus norvegicus | Mutation(s): 1 Gene Names: Gnai1, Gnai-1 EC: 3.6.5 |  | |
UniProt | |||||
Find proteins for P10824 (Rattus norvegicus) Explore P10824 Go to UniProtKB: P10824 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P10824 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GSP Query on GSP | B [auth A] | 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE C10 H16 N5 O13 P3 S XOFLBQFBSOEHOG-UUOKFMHZSA-N |  | ||
| MG Query on MG | C [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.07 Å
- R-Value Free: 0.197
- R-Value Work: 0.165
- R-Value Observed: 0.168
- Space Group: P 32 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 79.558 | α = 90 |
| b = 79.558 | β = 90 |
| c = 105.421 | γ = 120 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2019-08-21 Deposition Author(s): Mascarenhas, R., Goossens, J., Leverson, B., Kothawala, S., Ballicora, M., Olsen, K., de freitas, D., Liu, D.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | R15GM112025 |
Revision History (Full details and data files)
- Version 1.0: 2019-08-21
Type: Initial release - Version 1.1: 2020-01-01
Changes: Author supporting evidence - Version 1.2: 2024-03-13
Changes: Advisory, Data collection, Database references, Derived calculations
