Download MendeleyCrystal structure of the cofactor-free Aspergillus flavus urate oxidase T57A variant anaerobically complexed with 9-methyl uric acid
Bui, S., Lowden, S.R.J., Steiner, R.A.To be published.
6RGT
Crystal structure of the cofactor-free Aspergillus flavus urate oxidase T57A variant anaerobically complexed with 9-methyl uric acid
- PDB DOI: https://doi.org/10.2210/pdb6RGT/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Aspergillus flavus
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2019-04-17 Released: 2020-05-13
- Funding Organization(s): Biotechnology and Biological Sciences Research Council
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.60 Å
- R-Value Free: 0.189
- R-Value Work: 0.162
- R-Value Observed: 0.163
Starting Model: experimental
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This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Uricase | 302 | Aspergillus flavus | Mutation(s): 1 Gene Names: uaZ, uox EC: 1.7.3.3 |  | |
UniProt | |||||
Find proteins for Q00511 (Aspergillus flavus) Explore Q00511 Go to UniProtKB: Q00511 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q00511 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MUA Query on MUA | B [auth A] | 9-METHYL URIC ACID C6 H6 N4 O3 XJEJWDFDVPDMAS-UHFFFAOYSA-N |  | ||
| TRS Query on TRS | D [auth A], E [auth A] | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL C4 H12 N O3 LENZDBCJOHFCAS-UHFFFAOYSA-O |  | ||
| MPD Query on MPD | C [auth A] | (4S)-2-METHYL-2,4-PENTANEDIOL C6 H14 O2 SVTBMSDMJJWYQN-YFKPBYRVSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.60 Å
- R-Value Free: 0.189
- R-Value Work: 0.162
- R-Value Observed: 0.163
- Space Group: I 2 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 79.291 | α = 90 |
| b = 95.116 | β = 90 |
| c = 104.503 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| CrysalisPro | data reduction |
| Aimless | data scaling |
| REFMAC | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2020-05-13 Deposition Author(s): Bui, S., Lowden, S.R.J., Steiner, R.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Biotechnology and Biological Sciences Research Council | United Kingdom | BB/P000169/1 |
Revision History (Full details and data files)
- Version 1.0: 2020-05-13
Type: Initial release - Version 1.1: 2024-01-24
Changes: Data collection, Database references, Refinement description
