Download MendeleyStructural evidence for a latch mechanism regulating access to the active site of SufS-family cysteine desulfurases
Dunkle, J.A., Bruno, M.R., Frantom, P.A.(2020) Acta Crystallogr D Biol Crystallogr 76: 291-301
6UY5
E. coli cysteine desulfurase SufS with a spontaneously rotated beta-hairpin
- PDB DOI: https://doi.org/10.2210/pdb6UY5/pdb
- Classification: Lyase, Transferase
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2019-11-11 Released: 2020-03-25
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.221
- R-Value Work: 0.197
- R-Value Observed: 0.197
Starting Model: experimental
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This is version 1.2 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Cysteine desulfurase | 406 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: sufS, csdB, ynhB, b1680, JW1670 EC: 2.8.1.7 (PDB Primary Data), 4.4.1.16 (PDB Primary Data), 3.13.1 (UniProt) |  | |
UniProt | |||||
Find proteins for P77444 (Escherichia coli (strain K12)) Explore P77444 Go to UniProtKB: P77444 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P77444 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PLP Query on PLP | C [auth A], D [auth B] | PYRIDOXAL-5'-PHOSPHATE C8 H10 N O6 P NGVDGCNFYWLIFO-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| CSS Query on CSS | A, B | L-PEPTIDE LINKING | C3 H7 N O2 S2 |  | CYS |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.221
- R-Value Work: 0.197
- R-Value Observed: 0.197
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 60.54 | α = 90 |
| b = 115.74 | β = 114.47 |
| c = 63.66 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XDS | data reduction |
| XSCALE | data scaling |
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2020-03-25 Deposition Author(s): Dunkle, J.A., Frantom, P.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | GM112919 |
Revision History (Full details and data files)
- Version 1.0: 2020-03-25
Type: Initial release - Version 1.1: 2023-10-11
Changes: Data collection, Database references, Derived calculations, Refinement description - Version 1.2: 2023-11-15
Changes: Data collection
