Generalized enzymatic mechanism of catalysis by tetrameric L-asparaginases from mesophilic bacteria.
Strzelczyk, P., Zhang, D., Dyba, M., Wlodawer, A., Lubkowski, J.(2020) Sci Rep 10: 17516
Experimental Data Snapshot
Starting Model: experimental
View more details
(2020) Sci Rep 10: 17516
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
Glutaminase-asparaginase | 337 | Pseudomonas putida KT2440 | Mutation(s): 0  Gene Names: ansB, PP_2453 EC: 3.5.1.38 | ||
UniProt | |||||
Find proteins for Q88K39 (Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)) Explore Q88K39  Go to UniProtKB:  Q88K39 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | Q88K39 | ||||
Sequence AnnotationsExpand | |||||
|
Ligands 1 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
ASP (Subject of Investigation/LOI) Query on ASP | E [auth A], F [auth B], G [auth C], H [auth D] | ASPARTIC ACID C4 H7 N O4 CKLJMWTZIZZHCS-REOHCLBHSA-N |
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 80.728 | α = 90 |
b = 80.728 | β = 90 |
c = 176.401 | γ = 120 |
Software Name | Purpose |
---|---|
REFMAC | refinement |
PDB_EXTRACT | data extraction |
HKL-3000 | data reduction |
HKL-3000 | data scaling |
PHASER | phasing |
Funding Organization | Location | Grant Number |
---|---|---|
National Institutes of Health/National Cancer Institute (NIH/NCI) | United States | -- |