7CY9

Crystal structure of a biodegradable plastic-degrading cutinase from Paraphoma sp. B47-9 solved by getting the phase from anomalous scattering of uncovalently coordinated arsenic (cacodylate).


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.36 Å
  • R-Value Free: 0.166 
  • R-Value Work: 0.122 
  • R-Value Observed: 0.124 

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Ligand Structure Quality Assessment 


This is version 2.0 of the entry. See complete history


Literature

Crystal structure of a biodegradable plastic-degrading cutinase from Paraphoma sp. B47-9 solved by getting the phase from anomalous scattering of uncovalently coordinated arsenic (cacodylate).

Suzuki, K.Koitabashi, M.

To be published.

Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Cutinase
A, B
195Paraphoma sp. B47-9Mutation(s): 0 
EC: 3.1.1.74
UniProt
Find proteins for A0A060N399 (Paraphoma sp. B47-9)
Explore A0A060N399 
Go to UniProtKB:  A0A060N399
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupA0A060N399
Sequence Annotations
Expand
  • Reference Sequence
Small Molecules
Modified Residues  1 Unique
IDChains TypeFormula2D DiagramParent
PCA
Query on PCA
A, B
L-PEPTIDE LINKINGC5 H7 N O3GLN
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.36 Å
  • R-Value Free: 0.166 
  • R-Value Work: 0.122 
  • R-Value Observed: 0.124 
  • Space Group: P 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 34.886α = 103.78
b = 43.517β = 92.42
c = 51.724γ = 101.49
Software Package:
Software NamePurpose
REFMACrefinement
Aimlessdata scaling
SHELXphasing
PDB_EXTRACTdata extraction
xia2data reduction

Structure Validation

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Ligand Structure Quality Assessment 


Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2020-09-30
    Type: Initial release
  • Version 2.0: 2020-12-16
    Type: Coordinate replacement
    Reason: Model orientation/position
    Changes: Advisory, Atomic model, Data collection, Database references, Derived calculations, Refinement description, Structure summary