Crystal structure of a biodegradable plastic-degrading cutinase from Paraphoma sp. B47-9 solved by getting the phase from anomalous scattering of uncovalently coordinated arsenic (cacodylate).
Suzuki, K., Koitabashi, M.To be published.
Experimental Data Snapshot
Entity ID: 1 | |||||
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Molecule | Chains | Sequence Length | Organism | Details | Image |
Cutinase | 195 | Paraphoma sp. B47-9 | Mutation(s): 0  EC: 3.1.1.74 | ||
UniProt | |||||
Find proteins for A0A060N399 (Paraphoma sp. B47-9) Explore A0A060N399  Go to UniProtKB:  A0A060N399 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | A0A060N399 | ||||
Sequence AnnotationsExpand | |||||
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Ligands 2 Unique | |||||
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ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
CAD (Subject of Investigation/LOI) Query on CAD | C [auth A], D [auth A], G [auth B] | CACODYLIC ACID C2 H7 As O2 OGGXGZAMXPVRFZ-UHFFFAOYSA-N | |||
NA Query on NA | E [auth A], F [auth A], H [auth B], I [auth B] | SODIUM ION Na FKNQFGJONOIPTF-UHFFFAOYSA-N |
Modified Residues 1 Unique | |||||
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ID | Chains | Type | Formula | 2D Diagram | Parent |
PCA Query on PCA | A, B | L-PEPTIDE LINKING | C5 H7 N O3 | GLN |
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 34.886 | α = 103.78 |
b = 43.517 | β = 92.42 |
c = 51.724 | γ = 101.49 |
Software Name | Purpose |
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REFMAC | refinement |
Aimless | data scaling |
SHELX | phasing |
PDB_EXTRACT | data extraction |
xia2 | data reduction |