7KYM

Crystal structure of the MarR family transcriptional regulator from Bradyrhizobium japonicum


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.50 Å
  • R-Value Free: 0.210 
  • R-Value Work: 0.191 

Starting Model: experimental
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wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome.

Conway, J.M.Walton, W.G.Salas-Gonzalez, I.Law, T.F.Lindberg, C.A.Crook, L.E.Kosina, S.M.Fitzpatrick, C.R.Lietzan, A.D.Northen, T.R.Jones, C.D.Finkel, O.M.Redinbo, M.R.Dangl, J.L.

(2022) Nat Microbiol 7: 1817-1833

  • DOI: https://doi.org/10.1038/s41564-022-01244-3
  • Primary Citation of Related Structures:  
    7KFO, 7KFQ, 7KFS, 7KH3, 7KIG, 7KJL, 7KJQ, 7KK0, 7KKC, 7KKI, 7KRH, 7KUA, 7KYM, 7L19, 7L1I

  • PubMed Abstract: 

    Chemical signalling in the plant microbiome can have drastic effects on microbial community structure, and on host growth and development. Previously, we demonstrated that the auxin metabolic signal interference performed by the bacterial genus Variovorax via an auxin degradation locus was essential for maintaining stereotypic root development in an ecologically relevant bacterial synthetic community. Here, we dissect the Variovorax auxin degradation locus to define the genes iadDE as necessary and sufficient for indole-3-acetic acid (IAA) degradation and signal interference. We determine the crystal structures and binding properties of the operon's MarR-family repressor with IAA and other auxins. Auxin degradation operons were identified across the bacterial tree of life and we define two distinct types on the basis of gene content and metabolic products: iac-like and iad-like. The structures of MarRs from representatives of each auxin degradation operon type establish that each has distinct IAA-binding pockets. Comparison of representative IAA-degrading strains from diverse bacterial genera colonizing Arabidopsis plants show that while all degrade IAA, only strains containing iad-like auxin-degrading operons interfere with auxin signalling in a complex synthetic community context. This suggests that iad-like operon-containing bacterial strains, including Variovorax species, play a key ecological role in modulating auxins in the plant microbiome.


  • Organizational Affiliation

    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
MarR family transcriptional regulator
A, B
184Bradyrhizobium japonicumMutation(s): 0 
Gene Names: BJA01nite_12290MA20_13490
UniProt
Find proteins for A0A0A3XYM8 (Bradyrhizobium japonicum)
Explore A0A0A3XYM8 
Go to UniProtKB:  A0A0A3XYM8
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupA0A0A3XYM8
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.50 Å
  • R-Value Free: 0.210 
  • R-Value Work: 0.191 
  • Space Group: P 21 21 21
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 47.265α = 90
b = 62.125β = 90
c = 92.763γ = 90
Software Package:
Software NamePurpose
PHENIXrefinement
XDSdata reduction
Aimlessdata scaling
PHENIXphasing

Structure Validation

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Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
National Science Foundation (NSF, United States)United StatesIOS-1917270

Revision History  (Full details and data files)

  • Version 1.0: 2021-12-15
    Type: Initial release
  • Version 1.1: 2022-11-23
    Changes: Database references
  • Version 1.2: 2023-10-25
    Changes: Data collection, Refinement description