7OBU

Crystal structure of holo-F210W mutant of Hydroxy ketone aldolase (SwHKA) from Sphingomonas wittichii RW1, with the active site in the resting and the active state


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.20 Å
  • R-Value Free: 0.157 
  • R-Value Work: 0.129 
  • R-Value Observed: 0.131 

Starting Model: experimental
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This is version 1.3 of the entry. See complete history


Literature

Substrate Induced Movement of the Metal Cofactor between Active and Resting State.

Marsden, S.R.Wijma, H.J.Mohr, M.K.F.Justo, I.Hagedoorn, P.L.Laustsen, J.Jeffries, C.M.Svergun, D.Mestrom, L.McMillan, D.G.G.Bento, I.Hanefeld, U.

(2022) Angew Chem Int Ed Engl 61: e202213338-e202213338

  • DOI: https://doi.org/10.1002/anie.202213338
  • Primary Citation of Related Structures:  
    7NNK, 7NR1, 7NUJ, 7O5I, 7O5R, 7O5V, 7O5W, 7O87, 7O9R, 7OBU, 8ADQ

  • PubMed Abstract: 

    Regulation of enzyme activity is vital for living organisms. In metalloenzymes, far-reaching rearrangements of the protein scaffold are generally required to tune the metal cofactor's properties by allosteric regulation. Here structural analysis of hydroxyketoacid aldolase from Sphingomonas wittichii RW1 (SwHKA) revealed a dynamic movement of the metal cofactor between two coordination spheres without protein scaffold rearrangements. In its resting state configuration (M 2+ R ), the metal constitutes an integral part of the dimer interface within the overall hexameric assembly, but sterical constraints do not allow for substrate binding. Conversely, a second coordination sphere constitutes the catalytically active state (M 2+ A ) at 2.4 Å distance. Bidentate coordination of a ketoacid substrate to M 2+ A affords the overall lowest energy complex, which drives the transition from M 2+ R to M 2+ A . While not described earlier, this type of regulation may be widespread and largely overlooked due to low occupancy of some of its states in protein crystal structures.


  • Organizational Affiliation

    Biokatalyse, Afdeling Biotechnologie, Technische Universiteit Delft, van der Maasweg 9, 2629HZ, Delft, The Netherlands.


Macromolecules
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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
HpcH/HpaI aldolase
A, B
252Rhizorhabdus wittichii RW1Mutation(s): 1 
Gene Names: Swit_5035
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.20 Å
  • R-Value Free: 0.157 
  • R-Value Work: 0.129 
  • R-Value Observed: 0.131 
  • Space Group: H 3
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 71.094α = 90
b = 71.094β = 90
c = 222.394γ = 120
Software Package:
Software NamePurpose
PHENIXrefinement
REFMACrefinement
XDSdata reduction
BIOMOLdata scaling
MOLREPphasing

Structure Validation

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Ligand Structure Quality Assessment 


Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2022-10-26
    Type: Initial release
  • Version 1.1: 2022-11-23
    Changes: Database references
  • Version 1.2: 2022-12-07
    Changes: Database references
  • Version 1.3: 2024-01-31
    Changes: Data collection, Refinement description