Download MendeleyDirected evolution unlocks oxygen reactivity for a nicotine-degrading flavoenzyme.
Dulchavsky, M., Mitra, R., Wu, K., Li, J., Boer, K., Liu, X., Zhang, Z., Vasquez, C., Clark, C.T., Funckes, K., Shankar, K., Bonnet-Zahedi, S., Siddiq, M., Sepulveda, Y., Suhandynata, R.T., Momper, J.D., Calabrese, A.N., George, O., Stull, F., Bardwell, J.C.A.(2023) Nat Chem Biol
- PubMed: 37770699
- DOI: https://doi.org/10.1038/s41589-023-01426-y
- Primary Citation of Related Structures:
8DQ7, 8DQ8, 8DSV - PubMed Abstract:
The flavoenzyme nicotine oxidoreductase (NicA2) is a promising injectable treatment to aid in the cessation of smoking, a behavior responsible for one in ten deaths worldwide. NicA2 acts by degrading nicotine in the bloodstream before it reaches the brain. Clinical use of NicA2 is limited by its poor catalytic activity in the absence of its natural electron acceptor CycN. Without CycN, NicA2 is instead oxidized slowly by dioxygen (O 2 ), necessitating unfeasibly large doses in a therapeutic setting. Here, we report a genetic selection strategy that directly links CycN-independent activity of NicA2 to growth of Pseudomonas putida S16. This selection enabled us to evolve NicA2 variants with substantial improvement in their rate of oxidation by O 2 . The encoded mutations cluster around a putative O 2 tunnel, increasing flexibility and accessibility to O 2 in this region. These mutations further confer desirable clinical properties. A variant form of NicA2 is tenfold more effective than the wild type at degrading nicotine in the bloodstream of rats.
Organizational Affiliation:
Howard Hughes Medical Institute and Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
