1AMO

THREE-DIMENSIONAL STRUCTURE OF NADPH-CYTOCHROME P450 REDUCTASE: PROTOTYPE FOR FMN-AND FAD-CONTAINING ENZYMES

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d1amob1	All beta proteins	Reductase/isomerase/elongation factor common domain	Riboflavin synthase domain-like	NADPH-cytochrome p450 reductase FAD-binding domain-like	NADPH-cytochrome p450 reductase	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
B	d1amob2	Alpha and beta proteins (a/b)	Flavodoxin-like	Flavoproteins	Cytochrome p450 reductase N-terminal domain-like	NADPH-cytochrome p450 reductase, N-terminal domain	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
B	d1amob3	Alpha and beta proteins (a/b)	Ferredoxin reductase-like, C-terminal NADP-linked domain	Ferredoxin reductase-like, C-terminal NADP-linked domain	NADPH-cytochrome p450 reductase-like	NADPH-cytochrome p450 reductase	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
A	d1amoa1	All beta proteins	Reductase/isomerase/elongation factor common domain	Riboflavin synthase domain-like	NADPH-cytochrome p450 reductase FAD-binding domain-like	NADPH-cytochrome p450 reductase	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
A	d1amoa2	Alpha and beta proteins (a/b)	Flavodoxin-like	Flavoproteins	Cytochrome p450 reductase N-terminal domain-like	NADPH-cytochrome p450 reductase, N-terminal domain	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
A	d1amoa3	Alpha and beta proteins (a/b)	Ferredoxin reductase-like, C-terminal NADP-linked domain	Ferredoxin reductase-like, C-terminal NADP-linked domain	NADPH-cytochrome p450 reductase-like	NADPH-cytochrome p450 reductase	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	Flavoproteins	8034257	3001217	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ferredoxin reductase-like, C-terminal NADP-linked domain	8034259	3001461	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Riboflavin synthase domain-like	8034256	3001812	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Flavoproteins	8034257	3001217	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Riboflavin synthase domain-like	8034256	3001812	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Ferredoxin reductase-like, C-terminal NADP-linked domain	8034259	3001461	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	FAD_binding_1_1st	e1amoB5	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: FAD_binding_1_1st	ECOD (1.6)
B	FAD_binding_1_2nd	e1amoB4	A: alpha bundles	X: NADPH-cytochrome p450 reductase helical insertion domain (From Topology)	H: NADPH-cytochrome p450 reductase helical insertion domain (From Topology)	T: NADPH-cytochrome p450 reductase helical insertion domain	F: FAD_binding_1_2nd	ECOD (1.6)
B	Flavodoxin_1	e1amoB2	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Flavoproteins/Phosphotyrosine protein phosphatases-like	T: Flavoproteins	F: Flavodoxin_1	ECOD (1.6)
B	NAD_binding_1	e1amoB3	A: a/b three-layered sandwiches	X: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	H: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	T: Ferredoxin reductase-like, C-terminal NADP-linked domain	F: NAD_binding_1	ECOD (1.6)
A	FAD_binding_1_1st	e1amoA5	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: FAD_binding_1_1st	ECOD (1.6)
A	FAD_binding_1_2nd	e1amoA4	A: alpha bundles	X: NADPH-cytochrome p450 reductase helical insertion domain (From Topology)	H: NADPH-cytochrome p450 reductase helical insertion domain (From Topology)	T: NADPH-cytochrome p450 reductase helical insertion domain	F: FAD_binding_1_2nd	ECOD (1.6)
A	Flavodoxin_1	e1amoA2	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Flavoproteins/Phosphotyrosine protein phosphatases-like	T: Flavoproteins	F: Flavodoxin_1	ECOD (1.6)
A	NAD_binding_1	e1amoA3	A: a/b three-layered sandwiches	X: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	H: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	T: Ferredoxin reductase-like, C-terminal NADP-linked domain	F: NAD_binding_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	3.40.50.360	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Flavodoxin domain	CATH (4.3.0)
B	2.40.30.10	Mainly Beta	Beta Barrel	Elongation Factor Tu (Ef-tu)	domain 3	CATH (4.3.0)
B	1.20.990.10	Mainly Alpha	Up-down Bundle	NADPH-cytochrome p450 Reductase	Chain A, domain 3	CATH (4.3.0)
B	3.40.50.80	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module	CATH (4.3.0)
A	3.40.50.360	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Flavodoxin domain	CATH (4.3.0)
A	2.40.30.10	Mainly Beta	Beta Barrel	Elongation Factor Tu (Ef-tu)	domain 3	CATH (4.3.0)
A	1.20.990.10	Mainly Alpha	Up-down Bundle	NADPH-cytochrome p450 Reductase	Chain A, domain 3	CATH (4.3.0)
A	3.40.50.80	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00667	FAD binding domain (FAD_binding_1)	FAD binding domain	This domain is found in sulfite reductase, NADPH cytochrome P450 reductase, Nitric oxide synthase and methionine synthase reductase.	Domain
A, B	PF00175	Oxidoreductase NAD-binding domain (NAD_binding_1)	Oxidoreductase NAD-binding domain	Xanthine dehydrogenases, that also bind FAD/NAD, have essentially no similarity.	Domain
A, B	PF00258	Flavodoxin (Flavodoxin_1)	Flavodoxin	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	NADPH-CYTOCHROME P450 REDUCTASE	oxidoreductase activity catalytic activity binding nucleotide binding FMN binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding flavin adenine dinucleotide binding oxidoreductase activity catalytic activity binding nucleotide binding FMN binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding flavin adenine dinucleotide binding cytochrome-b5 reductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor hydrolase activity electron transfer activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen nitric oxide dioxygenase NAD(P)H activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of two atoms of oxygen into one donor dioxygenase activity purine nucleotide binding adenyl nucleotide binding NADP binding NADPH-hemoprotein reductase activity iron-cytochrome-c reductase activity oxidoreductase activity, acting on a heme group of donors protein binding enzyme binding	response to stimulus response to chemical response to xenobiotic stimulus nitric oxide metabolic process catabolic process nitric oxide catabolic process metabolic process reactive nitrogen species metabolic process cellular response to nitrogen compound cellular response to stimulus response to endogenous stimulus response to peptide hormone cellular response to peptide hormone stimulus response to oxygen-containing compound response to stimulus response to chemical response to xenobiotic stimulus nitric oxide metabolic process catabolic process nitric oxide catabolic process metabolic process reactive nitrogen species metabolic process cellular response to nitrogen compound cellular response to stimulus response to endogenous stimulus response to peptide hormone cellular response to peptide hormone stimulus response to oxygen-containing compound response to nitrogen compound cellular response to oxygen-containing compound cellular response to endogenous stimulus cellular response to chemical stimulus response to hormone cellular process cellular response to hormone stimulus regulation of biological process regulation of cellular process negative regulation of programmed cell death regulation of apoptotic process biological regulation negative regulation of biological process regulation of programmed cell death cell death negative regulation of apoptotic process negative regulation of cellular process apoptotic process programmed cell death regulation of steroid biosynthetic process regulation of cholesterol biosynthetic process regulation of cholesterol metabolic process organic hydroxy compound metabolic process sterol metabolic process steroid metabolic process regulation of lipid metabolic process positive regulation of biological process cholesterol metabolic process lipid biosynthetic process alcohol metabolic process regulation of primary metabolic process positive regulation of metabolic process regulation of small molecule metabolic process positive regulation of biosynthetic process positive regulation of lipid metabolic process small molecule metabolic process steroid biosynthetic process regulation of biosynthetic process secondary alcohol biosynthetic process regulation of alcohol biosynthetic process small molecule biosynthetic process regulation of steroid metabolic process cholesterol biosynthetic process positive regulation of cholesterol biosynthetic process primary metabolic process secondary alcohol metabolic process positive regulation of steroid biosynthetic process biosynthetic process sterol biosynthetic process positive regulation of lipid biosynthetic process regulation of sterol biosynthetic process organic hydroxy compound biosynthetic process alcohol biosynthetic process positive regulation of cholesterol metabolic process positive regulation of steroid metabolic process regulation of lipid biosynthetic process positive regulation of small molecule metabolic process regulation of metabolic process positive regulation of alcohol biosynthetic process positive regulation of sterol biosynthetic process lipid metabolic process positive regulation of cell communication positive regulation of signal transduction signaling regulation of signal transduction cell communication positive regulation of response to stimulus positive regulation of smoothened signaling pathway signal transduction positive regulation of cellular process regulation of smoothened signaling pathway regulation of signaling cell surface receptor signaling pathway smoothened signaling pathway regulation of response to stimulus regulation of cell communication positive regulation of signaling multicellular organismal process regulation of cartilage development regulation of chondrocyte differentiation regulation of developmental process connective tissue development cellular developmental process regulation of cell differentiation positive regulation of chondrocyte differentiation positive regulation of developmental process multicellular organism development skeletal system development positive regulation of cell differentiation cell differentiation anatomical structure development regulation of multicellular organismal process system development developmental process regulation of multicellular organismal development animal organ development tissue development chondrocyte differentiation positive regulation of multicellular organismal process positive regulation of cartilage development cartilage development demethylation cellular response to gonadotropin stimulus response to gonadotropin developmental growth endochondral bone morphogenesis organ growth growth plate cartilage chondrocyte proliferation regulation of growth plate cartilage chondrocyte proliferation growth plate cartilage development endochondral bone growth animal organ morphogenesis bone development cell population proliferation growth anatomical structure morphogenesis regulation of cell population proliferation bone growth skeletal system morphogenesis cartilage development involved in endochondral bone morphogenesis bone morphogenesis positive regulation of steroid hormone biosynthetic process regulation of hormone biosynthetic process cellular biosynthetic process regulation of hormone metabolic process positive regulation of cellular metabolic process regulation of biological quality hormone biosynthetic process regulation of steroid hormone biosynthetic process positive regulation of hormone metabolic process regulation of hormone levels cellular metabolic process regulation of cellular metabolic process hormone metabolic process regulation of cellular biosynthetic process positive regulation of hormone biosynthetic process positive regulation of cellular biosynthetic process electron transport chain generation of precursor metabolites and energy response to ketone response to lipid response to glucocorticoid response to corticosteroid response to steroid hormone response to dexamethasone response to organic cyclic compound oxoacid metabolic process nitrate catabolic process organic acid metabolic process nitrate metabolic process flavonoid metabolic process cellular response to follicle-stimulating hormone stimulus response to follicle-stimulating hormone fatty acid metabolic process carboxylic acid metabolic process lipid modification fatty acid oxidation cellular lipid metabolic process lipid oxidation monocarboxylic acid metabolic process cellular organofluorine metabolic process cellular organohalogen metabolic process carnitine metabolic process amino-acid betaine metabolic process cellular modified amino acid metabolic process response to nutrient levels response to nutrient	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle intracellular organelle organelle intracellular membrane-bounded organelle organelle subcompartment membrane endomembrane system endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle intracellular organelle organelle intracellular membrane-bounded organelle organelle subcompartment membrane endomembrane system endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum organelle membrane endoplasmic reticulum membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	Domain
A, B	IPR001433	Oxidoreductase FAD/NAD(P)-binding	Domain
A, B	IPR003097	Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding	Domain
A, B	IPR017938	Riboflavin synthase-like beta-barrel	Homologous Superfamily
A, B	IPR001094	Flavodoxin-like	Domain
A, B	IPR023173	NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily	Homologous Superfamily
A, B	IPR008254	Flavodoxin/nitric oxide synthase	Domain
A, B	IPR023208	NADPH-cytochrome P450 reductase	Family
A, B	IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	Homologous Superfamily
A, B	IPR017927	FAD-binding domain, ferredoxin reductase-type	Domain
A, B	IPR029039	Flavoprotein-like superfamily	Homologous Superfamily

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	NADPH-hemoprotein reductase M-CSA #117	Rat NADPH-cytochrome P450 oxidoreductase (CYPOR) catalyses the transfer of electrons from NADPH to a range of cytochrome P450 proteins. Other electron acceptors include heme oxygenase, cytochrome b5 and fatty-acid elongase. It is a 78kDa protein and is attached to the cytoplasmic side of the endoplasmic reticulum and the outer membrane of the nuclear envelope by a hydrophobic N-terminal membrane anchor. The FAD and FMN containing domains are linked by a flexible peptide hinge and it is thought that CYPOR evolved after a fusion of genes encoding for a FMN-containing flavodoxin and a FAD- binding ferredoxin-NADP reductase.	Defined by 4 residues: SER:A-394 [auth A-457]CYS:A-567 [auth A-630]ASP:A-612 [auth A-675]TRP:A-614 [auth A-677] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 1.6.2.4 (PDB Primary Data) Search M-CSA Motif + EC 1.6.2.4

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.