1DUG

STRUCTURE OF THE FIBRINOGEN G CHAIN INTEGRIN BINDING AND FACTOR XIIIA CROSSLINKING SITES OBTAINED THROUGH CARRIER PROTEIN DRIVEN CRYSTALLIZATION

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d1dugb1	All alpha proteins	GST C-terminal domain-like	GST C-terminal domain-like	Glutathione S-transferase (GST), C-terminal domain	Class alpha GST	(Schistosoma japonicum ) [TaxId: 6182 ], Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d1dugb2	Alpha and beta proteins (a/b)	Thioredoxin fold	Thioredoxin-like	Glutathione S-transferase (GST), N-terminal domain	Class alpha GST	(Schistosoma japonicum ) [TaxId: 6182 ], Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d1duga1	All alpha proteins	GST C-terminal domain-like	GST C-terminal domain-like	Glutathione S-transferase (GST), C-terminal domain	Class alpha GST	(Schistosoma japonicum ) [TaxId: 6182 ], Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d1duga2	Alpha and beta proteins (a/b)	Thioredoxin fold	Thioredoxin-like	Glutathione S-transferase (GST), N-terminal domain	Class alpha GST	(Schistosoma japonicum ) [TaxId: 6182 ], Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	GST C-terminal domain-like	8037552	3000305	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Thioredoxin-like	8044378	3000031	SCOP2B (2022-06-29)
A	SCOP2 Family	Glutathione S-transferase (GST), C-terminal domain	8025173	4000976	SCOP2 (2022-06-29)
A	SCOP2 Family	Glutathione S-transferase (GST), N-terminal domain	8032000	4000472	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	GST C-terminal domain-like	8037552	3000305	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Thioredoxin-like	8044378	3000031	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	GST_C_3	e1dugB1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Glutathione S-transferase (GST)-C (From Topology)	T: Glutathione S-transferase (GST)-C	F: GST_C_3	ECOD (1.6)
B	GST_N_5	e1dugB2	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: GST_N_5	ECOD (1.6)
A	GST_C_3	e1dugA1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Glutathione S-transferase (GST)-C (From Topology)	T: Glutathione S-transferase (GST)-C	F: GST_C_3	ECOD (1.6)
A	GST_N_5	e1dugA2	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: GST_N_5	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)
B	1.20.1050.10	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
A	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)
A	1.20.1050.10	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF14497	Glutathione S-transferase, C-terminal domain (GST_C_3)	Glutathione S-transferase, C-terminal domain	This domain is closely related to Pfam:PF00043.	Domain
A, B	PF02798	Glutathione S-transferase, N-terminal domain (GST_N)	Glutathione S-transferase, N-terminal domain	Function: conjugation of reduced glutathione to a variety of targets. Also included in the alignment, but not GSTs: S-crystallins from squid (similarity to GST previously noted); eukaryotic elongation factors 1-gamma (not known to have GST activity a ...	Function: conjugation of reduced glutathione to a variety of targets. Also included in the alignment, but not GSTs: S-crystallins from squid (similarity to GST previously noted); eukaryotic elongation factors 1-gamma (not known to have GST activity and similarity not previously recognised); HSP26 family of stress-related proteins including auxin-regulated proteins in plants and stringent starvation proteins in E. coli (not known to have GST activity and similarity not previously recognised). The glutathione molecule binds in a cleft between the N- and C-terminal domains - the catalytically important residues are proposed to reside in the N-terminal domain [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	chimera of GLUTATHIONE S-TRANSFERASE-synthetic LINKEr-C-TERMINAL FIBRINOGEN GAMMA CHAIN	glutathione transferase activity transferase activity transferase activity, transferring alkyl or aryl (other than methyl) groups catalytic activity protein binding binding signaling receptor binding cell adhesion molecule binding structural molecule activity extracellular matrix structural constituent cation binding ion binding metal ion binding small molecule binding glutathione transferase activity transferase activity transferase activity, transferring alkyl or aryl (other than methyl) groups catalytic activity protein binding binding signaling receptor binding cell adhesion molecule binding structural molecule activity extracellular matrix structural constituent cation binding ion binding metal ion binding small molecule binding identical protein binding	glutathione metabolic process cellular metabolic process metabolic process sulfur compound metabolic process cellular process amide metabolic process cellular modified amino acid metabolic process cell adhesion cell-matrix adhesion cell-substrate adhesion cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization glutathione metabolic process cellular metabolic process metabolic process sulfur compound metabolic process cellular process amide metabolic process cellular modified amino acid metabolic process cell adhesion cell-matrix adhesion cell-substrate adhesion cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular component organization or biogenesis developmental process cellular developmental process anatomical structure maturation platelet maturation cell differentiation anatomical structure development cell development cell maturation developmental maturation protein polymerization cellular response to stimulus positive regulation of cell communication regulation of cellular process positive regulation of signal transduction response to stimulus signaling positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus positive regulation of ERK1 and ERK2 cascade regulation of intracellular signal transduction intracellular signaling cassette signal transduction positive regulation of cellular process regulation of biological process regulation of signaling positive regulation of MAPK cascade biological regulation positive regulation of intracellular signal transduction regulation of MAPK cascade regulation of response to stimulus MAPK cascade regulation of cell communication ERK1 and ERK2 cascade regulation of ERK1 and ERK2 cascade positive regulation of signaling regulation of tube diameter multicellular organismal process system process regulation of tube size regulation of system process positive regulation of vasoconstriction circulatory system process regulation of blood circulation vascular process in circulatory system regulation of vasoconstriction positive regulation of multicellular organismal process regulation of multicellular organismal process blood vessel diameter maintenance regulation of biological quality vasoconstriction blood circulation regulation of anatomical structure size response to metal ion response to chemical response to calcium ion extrinsic apoptotic signaling pathway via death domain receptors negative regulation of signaling negative regulation of biological process regulation of programmed cell death extrinsic apoptotic signaling pathway negative regulation of cell communication negative regulation of extrinsic apoptotic signaling pathway negative regulation of apoptotic signaling pathway negative regulation of cellular process cell surface receptor signaling pathway negative regulation of response to stimulus negative regulation of programmed cell death regulation of apoptotic process negative regulation of extrinsic apoptotic signaling pathway via death domain receptors cell death negative regulation of signal transduction regulation of extrinsic apoptotic signaling pathway apoptotic signaling pathway negative regulation of apoptotic process regulation of extrinsic apoptotic signaling pathway via death domain receptors apoptotic process programmed cell death regulation of apoptotic signaling pathway regulation of localization transport positive regulation of secretion by cell positive regulation of exocytosis vesicle-mediated transport localization regulation of secretion by cell secretion by cell positive regulation of secretion regulation of transport secretion export from cell regulation of vesicle-mediated transport establishment of localization regulation of secretion positive regulation of transport exocytosis regulation of exocytosis regulation of body fluid levels regulation of response to external stimulus regulation of response to wounding regulation of coagulation response to stress regulation of response to stress coagulation response to wounding regulation of hemostasis wound healing negative regulation of wound healing regulation of blood coagulation negative regulation of blood coagulation hemostasis response to external stimulus negative regulation of response to wounding blood coagulation negative regulation of hemostasis fibrinolysis negative regulation of coagulation negative regulation of response to external stimulus regulation of wound healing negative regulation of multicellular organismal process cellular biosynthetic process protein maturation blood coagulation, fibrin clot formation protein metabolic process protein activation cascade primary metabolic process gene expression macromolecule biosynthetic process biosynthetic process macromolecule metabolic process homotypic cell-cell adhesion cell activation platelet aggregation cell-cell adhesion platelet activation zymogen activation proteolysis protein processing plasminogen activation positive regulation of substrate adhesion-dependent cell spreading regulation of cell-substrate adhesion positive regulation of cell adhesion regulation of substrate adhesion-dependent cell spreading regulation of cell adhesion positive regulation of cell-substrate adhesion substrate adhesion-dependent cell spreading protein localization establishment of protein localization cellular localization nitrogen compound transport regulation of protein localization positive regulation of establishment of protein localization protein localization to extracellular region positive regulation of protein transport cellular macromolecule localization regulation of establishment of protein localization establishment of protein localization to extracellular region regulation of protein transport protein secretion positive regulation of protein secretion regulation of protein secretion protein transport regulation of cellular localization macromolecule localization positive regulation of protein localization response to peptide response to interleukin-6 cellular response to interleukin-6 response to cytokine cellular response to cytokine stimulus positive regulation of cell-cell adhesion heterotypic cell-cell adhesion positive regulation of heterotypic cell-cell adhesion regulation of cell-cell adhesion regulation of heterotypic cell-cell adhesion negative regulation of endothelial cell apoptotic process endothelial cell apoptotic process regulation of endothelial cell apoptotic process regulation of hormone secretion regulation of peptide secretion hormone secretion peptide secretion positive regulation of hormone secretion amide transport signal release regulation of peptide hormone secretion positive regulation of peptide secretion regulation of hormone levels positive regulation of peptide hormone secretion regulation of peptide transport cell-cell signaling peptide transport peptide hormone secretion hormone transport cellular response to interleukin-1 response to interleukin-1	protein-containing complex extracellular region extracellular space cellular anatomical entity fibrinogen complex cell periphery cell surface membrane plasma membrane external side of plasma membrane side of membrane extracellular exosome membrane-bounded organelle extracellular vesicle protein-containing complex extracellular region extracellular space cellular anatomical entity fibrinogen complex cell periphery cell surface membrane plasma membrane external side of plasma membrane side of membrane extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle organelle vesicle extracellular membrane-bounded organelle extracellular matrix external encapsulating structure collagen-containing extracellular matrix blood microparticle intracellular anatomical structure membrane-enclosed lumen intracellular membrane-bounded organelle endomembrane system intracellular organelle lumen endoplasmic reticulum lumen intracellular organelle organelle lumen endoplasmic reticulum cytoplasm intracellular vesicle cytoplasmic vesicle secretory granule secretory vesicle platelet alpha granule vesicle lumen cytoplasmic vesicle lumen secretory granule lumen platelet alpha granule lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR004046	Glutathione S-transferase, C-terminal	Domain
A, B	IPR050213	Glutathione S-transferase superfamily	Family
A, B	IPR010987	Glutathione S-transferase, C-terminal-like	Domain
A, B	IPR036282	Glutathione S-transferase, C-terminal domain superfamily	Homologous Superfamily
A, B	IPR004045	Glutathione S-transferase, N-terminal	Domain
A, B	IPR036249	Thioredoxin-like superfamily	Homologous Superfamily
A, B	IPR040079	Glutathione transferase family	Family
A, B	IPR037579	Fibrinogen/angiopoietin-like	Family
A, B	IPR012290	Fibrinogen, alpha/beta/gamma chain, coiled coil domain	Domain
A, B	IPR020837	Fibrinogen, conserved site	Conserved Site
A, B	IPR014716	Fibrinogen, alpha/beta/gamma chain, C-terminal globular, subdomain 1	Homologous Superfamily
A, B	IPR002181	Fibrinogen, alpha/beta/gamma chain, C-terminal globular domain	Domain
A, B	IPR036056	Fibrinogen-like, C-terminal	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P02679	congenital afibrinogenemia familial dysfibrinogenemia keloid Dysfibrinogenemia, Congenital vascular disease adenoma gastric cancer lung carcinoma pancreatic ductal adenocarcinoma cystic fibrosis familial hypofibrinogenemia gastric neoplasm sarcoidosis hereditary diffuse gastric adenocarcinoma lung cancer congenital afibrinogenemia familial dysfibrinogenemia keloid Dysfibrinogenemia, Congenital vascular disease adenoma gastric cancer lung carcinoma pancreatic ductal adenocarcinoma cystic fibrosis familial hypofibrinogenemia gastric neoplasm sarcoidosis hereditary diffuse gastric adenocarcinoma lung cancer non-small cell lung carcinoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.